2例肺结核合并HIV抗体不确定样本的跟踪检测
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摘要
目的通过2例肺结核合并HIV抗体不确定样本的跟踪检测,探讨对感染合并不确定样本进行核酸检测的必要性,为检测策略的完善和应用推广提供依据。方法对2例肺结核合并HIV抗体不确定者跟踪检测,在首次、4周、8周分别进行3次HIV抗体确证检测,在第8周同时进行病毒核酸检测。结果病例1:3次HIV抗体确证试验,带型均不能判定为阳性,第3次同时进行病毒核酸定性为阴性,判定为HIV-1抗体阴性。病例2:3次HIV抗体确证试验,带型在第3次出现进展,核酸定性阳性,核酸定量12 000 copy/ml,判定为HIV-1抗体阳性。结论对感染合并HIV抗体不确定样本,进行核酸检测进一步明确诊断非常必要。
Objective Through tracking and detecting 2 case of uncertain antibodies in pulmonary tuberculosis complicated with HIV,to explore the necessity of nucleic acid detection for uncertain samples with infection so as to provide basis for improving detection strategy and application. Methods Following up detection and tracking was performed on the two uncertain antibodies with pulmonary tuberculosis infection complicated with HIV. 3 HIV antibody confirmatory tests were performed for the first time,4 weeks,and 8 weeks,and viral nucleic acid was detected at the eighth week. Results Case one underwent 3 HIV antibody confirmatory tests,belt type could not be determined as positive,qualitative and quantitative detection of viral nucleic acid was performed at the third time,without response and TND,and diagnozed as negative for HIV-1 antibodies. Case two underwent3 HIV antibody confirmatory tests,the belt type got progressed in the third time,and the nucleic acid was positive and the nucleic acid was 12 000 copy/ml. The diagnosis was positive for HIV-1 antibodies. Conclusion It is necessary to combine nucleic acid detection to make a further diagnosis for the infection with HIV antibody uncertainty samples.
Objective Through tracking and detecting 2 case of uncertain antibodies in pulmonary tuberculosis complicated with HIV,to explore the necessity of nucleic acid detection for uncertain samples with infection so as to provide basis for improving detection strategy and application. Methods Following up detection and tracking was performed on the two uncertain antibodies with pulmonary tuberculosis infection complicated with HIV. 3 HIV antibody confirmatory tests were performed for the first time,4 weeks,and 8 weeks,and viral nucleic acid was detected at the eighth week. Results Case one underwent 3 HIV antibody confirmatory tests,belt type could not be determined as positive,qualitative and quantitative detection of viral nucleic acid was performed at the third time,without response and TND,and diagnozed as negative for HIV-1 antibodies. Case two underwent3 HIV antibody confirmatory tests,the belt type got progressed in the third time,and the nucleic acid was positive and the nucleic acid was 12 000 copy/ml. The diagnosis was positive for HIV-1 antibodies. Conclusion It is necessary to combine nucleic acid detection to make a further diagnosis for the infection with HIV antibody uncertainty samples.
引文
[1]彭瑾瑜,贺健梅,江洋,等.湖南省2008-2012年HIV抗体不确定结果分析[J].中国艾滋病性病,2011,20(1):210-213.
[2]吴可可,李伟,虞成超,等.HIV-1抗体不确定标本的血清学特征与病毒载量[J].中国卫生检验杂志,2015,25(2):202-203,206.
[3]陈慧萍,刘雷,张连生,等.Logistic回归判别模型判别HIV抗体不确定者转归的可行性[J].中国艾滋病性病,2014,20(5):367-369,379.
[4]Ngan CC,Ai-Ee Ling,Li-See,et al.Cutting out spurios gp160bands from humam immunodificiency virus western blot results[J].J Clin Microbiol,2001,39(1):411.
[5]Syed IH,Balakrishnan P,Solomon SS,et al.HIV-1 westernblot assay:What deternines an indeterminate status[J].Indian J Med Sci,2005,59(10):443-450.
[6]Midthun K,Garrison L,Clements ML,et al.Frequency of indeterminate western-blot tests in healthy-adults at low-risk for human-immunodeficiency-virus infection[J].J Infect Dis,1990,162(6):1379-1382.
[7]黄晶晶.1例HIV抗体结果不确定原因的讨论[J].旅行医学科学,2008,14(4):39-40.
[8]杨育红,陈杰毅,陈明春.61份HIV抗体不确定样品的检测结果分析[J].中国卫生检验杂志,2014,24(16):2354-2355,2358.
[9]高敏,吴守丽,严延生.HIV抗体不确定的影响因素及其检测方法的研究进展[J].中国艾滋病性病,2016,22(4):308-310.
[10]王海,蒋文雅,顾叶松.HIV抗体不确定样本的特征与确证策略研究[J].中国卫生检验杂志,2013,23(15):3068-3071.
[11]高凯,文芳,徐慧芳,等.影响蛋白印迹法检测HIV抗体结果的生物学因素分析[J].热带医学杂志,2009,9(9):1073-1075.
[12]Dock NL,Lamberson HV,O'brien TA,et al.Evaluation of atypical human immunodeficiency virus immunoblot reactivity in blood donors[J].Transfusion,1988,28(5):412-418.
[13]Doran TI,Parra E.False-positive and indeterminate human immunodeficiency virus test results in preganantwomen[J].Arch Fam Med,2000,9(9):924-929.
[14]Araujo PR,Albertoni G,Arnoni C,et al.Rubella vaccination and transitory false-positive test results for human immunodeficiency virus Type 1 in blood donors[J].Transfusion,2009,49(11):2516-2517.
[15]段星,冯霞,王继宝.HIV抗体检测不确定时应用核酸诊断的可行性研究[J].中国艾滋病性病,2014,20(11):815-817.
[2]吴可可,李伟,虞成超,等.HIV-1抗体不确定标本的血清学特征与病毒载量[J].中国卫生检验杂志,2015,25(2):202-203,206.
[3]陈慧萍,刘雷,张连生,等.Logistic回归判别模型判别HIV抗体不确定者转归的可行性[J].中国艾滋病性病,2014,20(5):367-369,379.
[4]Ngan CC,Ai-Ee Ling,Li-See,et al.Cutting out spurios gp160bands from humam immunodificiency virus western blot results[J].J Clin Microbiol,2001,39(1):411.
[5]Syed IH,Balakrishnan P,Solomon SS,et al.HIV-1 westernblot assay:What deternines an indeterminate status[J].Indian J Med Sci,2005,59(10):443-450.
[6]Midthun K,Garrison L,Clements ML,et al.Frequency of indeterminate western-blot tests in healthy-adults at low-risk for human-immunodeficiency-virus infection[J].J Infect Dis,1990,162(6):1379-1382.
[7]黄晶晶.1例HIV抗体结果不确定原因的讨论[J].旅行医学科学,2008,14(4):39-40.
[8]杨育红,陈杰毅,陈明春.61份HIV抗体不确定样品的检测结果分析[J].中国卫生检验杂志,2014,24(16):2354-2355,2358.
[9]高敏,吴守丽,严延生.HIV抗体不确定的影响因素及其检测方法的研究进展[J].中国艾滋病性病,2016,22(4):308-310.
[10]王海,蒋文雅,顾叶松.HIV抗体不确定样本的特征与确证策略研究[J].中国卫生检验杂志,2013,23(15):3068-3071.
[11]高凯,文芳,徐慧芳,等.影响蛋白印迹法检测HIV抗体结果的生物学因素分析[J].热带医学杂志,2009,9(9):1073-1075.
[12]Dock NL,Lamberson HV,O'brien TA,et al.Evaluation of atypical human immunodeficiency virus immunoblot reactivity in blood donors[J].Transfusion,1988,28(5):412-418.
[13]Doran TI,Parra E.False-positive and indeterminate human immunodeficiency virus test results in preganantwomen[J].Arch Fam Med,2000,9(9):924-929.
[14]Araujo PR,Albertoni G,Arnoni C,et al.Rubella vaccination and transitory false-positive test results for human immunodeficiency virus Type 1 in blood donors[J].Transfusion,2009,49(11):2516-2517.
[15]段星,冯霞,王继宝.HIV抗体检测不确定时应用核酸诊断的可行性研究[J].中国艾滋病性病,2014,20(11):815-817.
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