454测序方法对人参种子内生真菌种类的研究
|
摘要
目的:研究人参内生真菌的生物多样性。方法:采用454测序技术对人参种子内生真菌进行分析。结果:测试的样品中,样品4与其他样品内生真菌区系差异较大,而其他四份样品之间存在的差异较小。子囊菌门真菌在人参种子内生真菌中占有绝对优势,平均百分比为32.7%;针对归类到属而言,共鉴定到属的种类为46个属,其中镰刀菌属序列数所占比列最大,为优势菌属。结论:人参种子中的内生真菌具有多样性,内生菌的分布在种子个体中也存在差异性。
Objective: To study the biodiversity of the endophytic fungi of ginseng seeds. Methods: Use 454 sequencing technology to analyze the diversity of endophytic fungi from the ginseng seeds. Results: Ascomycota had the overwhelming superiority,and its average percentage was 32. 7%. 46 genera were identified and Fusarium was the dominant genera. Conclusion: Endophytic fungi of Ginseng seeds has the biological diversity,and the distribution of the endophytic fungi of ginseng seeds has some differences.
Objective: To study the biodiversity of the endophytic fungi of ginseng seeds. Methods: Use 454 sequencing technology to analyze the diversity of endophytic fungi from the ginseng seeds. Results: Ascomycota had the overwhelming superiority,and its average percentage was 32. 7%. 46 genera were identified and Fusarium was the dominant genera. Conclusion: Endophytic fungi of Ginseng seeds has the biological diversity,and the distribution of the endophytic fungi of ginseng seeds has some differences.
引文
[1]张薇.人参根际土壤真菌与内生真菌的多样性及人参皂苷生物催化活性菌株的筛选[D].大连:辽宁师范大学,2011:2.
[2]张中朋,刘张林.2007年我国人参出口形势分析及展望[J].中国现代中药,2008,10(3):44-45.
[3]陈晖奇.茶树内生真菌的初步研究[D].福州:福建师范大学,2007:11-12.
[4]高剑.内生真菌多样性及其生态分布[D].湛江:广东海洋大学,2013:5-6.
[5]袁志林.疣粒野生稻(Oryza granulate)内生真菌资源挖掘、系统发育分析和功能初探[D].杭州:浙江大学,2010:6-7.
[6]吴令上.南方红豆杉内生真菌多样性、次生代谢产物及其与宿主的相关性研究[D].上海:第二军医大学,2012:71.
[7]王卫霞.新疆几种典型荒漠植物根际微生物特征及内生固氮菌的分离、促生性能研究[D].乌鲁木齐:新疆农业大学,2009:8-9.
[8]陈宝.内生细菌促进东南景天Zn/Cd的提取效应及其机制研究[D].杭州:浙江大学,2015:30-31.
[9]周永强,程玉鹏,刘丹丹,等.药用植物内生真菌代谢产物的活性研究进展[J].中医药信息,2014,31(3):158-161.
[10]程玉鹏,李天聪,林进华,等.内生真菌与药用植物宿主间的交流[J].中医药信息,2016,33(6):120-122.
[11]杨中铎,魏博,薛鹏辉.一株半夏内生青霉属真菌次级代谢产物及生物活性的研究[J].中医药学报,2015,43(2):27-30.
[12]孙景云,杨中铎,于海涛.雷公藤等10种植物分得49种内生真菌的发酵提取物杀虫活性研究[J].中医药学报,2016,44(6):9-12.
[13]徐姣,常越,刘振鹏,等.5种人参内生真菌对6种人参病害拮抗作用的研究[J].中医药学报,2016,44(6):16-18.
[14]Caporaso JG,Kuczynski J,Stombaugh J,et al.QIIME allows analysis of high-throughput community sequencing data[J].Nature Methods,2010,7(5):335-336.
[15]Schloss PD,Westcott SL,Ryabin T,et al.Introducing mothur:Opensource,platform-independent,community-supported software for describing and comparing microbial communities[J].Appl Environ Microbiol,2009,75(23):7537-7541.
[16]Edgar RC,Haas BJ,Clemente JC,et al.UCHIME improves sensitivity and speed of chimera detection[J].Bioinformatics,2011,27(16):2194-2200.
[17]Edgar RC.Search and clustering orders of magnitude faster than BLAST[J].Bioinformatics,2010,26(19):2460-2461.
[18]Amato KR,Yeoman CJ,Kent A,et al.Habitat degradation impacts black howler monkey(Alouatta pigra)gastrointestinal microbiomes[J].The ISME Journal,2013,7(7):1344-1353.
[19]Oberauner L,Zachow C,Lackner S,et al.The ignored diversity:complex bacterial communities in intensive care units revealed by 16S pyrosequencing[J].Scientific Reports,2013,3(3):1413.
[20]Huson DH,Mitra S,Ruscheweyh H,et al.Integrative analysis of environmental sequences using MEGAN4[J].Genome Research,2011,21(9):1552-1560.
[2]张中朋,刘张林.2007年我国人参出口形势分析及展望[J].中国现代中药,2008,10(3):44-45.
[3]陈晖奇.茶树内生真菌的初步研究[D].福州:福建师范大学,2007:11-12.
[4]高剑.内生真菌多样性及其生态分布[D].湛江:广东海洋大学,2013:5-6.
[5]袁志林.疣粒野生稻(Oryza granulate)内生真菌资源挖掘、系统发育分析和功能初探[D].杭州:浙江大学,2010:6-7.
[6]吴令上.南方红豆杉内生真菌多样性、次生代谢产物及其与宿主的相关性研究[D].上海:第二军医大学,2012:71.
[7]王卫霞.新疆几种典型荒漠植物根际微生物特征及内生固氮菌的分离、促生性能研究[D].乌鲁木齐:新疆农业大学,2009:8-9.
[8]陈宝.内生细菌促进东南景天Zn/Cd的提取效应及其机制研究[D].杭州:浙江大学,2015:30-31.
[9]周永强,程玉鹏,刘丹丹,等.药用植物内生真菌代谢产物的活性研究进展[J].中医药信息,2014,31(3):158-161.
[10]程玉鹏,李天聪,林进华,等.内生真菌与药用植物宿主间的交流[J].中医药信息,2016,33(6):120-122.
[11]杨中铎,魏博,薛鹏辉.一株半夏内生青霉属真菌次级代谢产物及生物活性的研究[J].中医药学报,2015,43(2):27-30.
[12]孙景云,杨中铎,于海涛.雷公藤等10种植物分得49种内生真菌的发酵提取物杀虫活性研究[J].中医药学报,2016,44(6):9-12.
[13]徐姣,常越,刘振鹏,等.5种人参内生真菌对6种人参病害拮抗作用的研究[J].中医药学报,2016,44(6):16-18.
[14]Caporaso JG,Kuczynski J,Stombaugh J,et al.QIIME allows analysis of high-throughput community sequencing data[J].Nature Methods,2010,7(5):335-336.
[15]Schloss PD,Westcott SL,Ryabin T,et al.Introducing mothur:Opensource,platform-independent,community-supported software for describing and comparing microbial communities[J].Appl Environ Microbiol,2009,75(23):7537-7541.
[16]Edgar RC,Haas BJ,Clemente JC,et al.UCHIME improves sensitivity and speed of chimera detection[J].Bioinformatics,2011,27(16):2194-2200.
[17]Edgar RC.Search and clustering orders of magnitude faster than BLAST[J].Bioinformatics,2010,26(19):2460-2461.
[18]Amato KR,Yeoman CJ,Kent A,et al.Habitat degradation impacts black howler monkey(Alouatta pigra)gastrointestinal microbiomes[J].The ISME Journal,2013,7(7):1344-1353.
[19]Oberauner L,Zachow C,Lackner S,et al.The ignored diversity:complex bacterial communities in intensive care units revealed by 16S pyrosequencing[J].Scientific Reports,2013,3(3):1413.
[20]Huson DH,Mitra S,Ruscheweyh H,et al.Integrative analysis of environmental sequences using MEGAN4[J].Genome Research,2011,21(9):1552-1560.
© 2004-2018 中国地质图书馆版权所有 京ICP备05064691号 京公网安备11010802017129号 地址：北京市海淀区学院路29号 邮编：100083 电话：办公室：(+86 10)66554848；文献借阅、咨询服务、科技查新：66554700 |