Thirty-two Sprague-Dawley rats were divided into four groups: control, I/R, MG132 control, and MG132 pretreatment. The I/R and MG132 pretreatment groups were subjected to mesenteric arterial ischemia for 1 h and reperfusion for 3 h. The control and MG132 control groups underwent surgical preparation including isolation of the superior mesenteric artery (SMA) without occlusion. The MG132 control and MG132 pretreatment groups were subjected to intraperitoneal administration of 0.5 mg/kg MG132 30 min before surgery. We collected serum specimens to measure TNF-伪, IL-6, liver tissue levels of malondialdehyde (MDA), AhR, and cyp1a2; NF魏B, I魏B伪, and ICAM-1 were also tested. Histologic changes of liver and intestine were subsequently evaluated.
Compared with the control group, significant increases in MDA, NF魏B, and ICAM-1 levels were accompanied by decreases in AhR, cyp1a2, and I魏B伪 expression in the liver in the I/R group, which is consistent with liver and intestinal tissue injury. MG132 blocked the alterations of the indicators above. There were no changes in the MG132 control group compared with the control group in the indicators above.
This study demonstrated that MG132 has a significant effect in protection against liver injury induced by intestinal I/R, which may be due to modulation of the AhR and NF魏B pathways.
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