Protein expression of hypoxia-inducible factor 1-alpha (HIF-1伪) in spot (Leiostomus xanthurus) exposed to constant and diel-cycling hypoxia

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• 作者：Mason J. Smith ; ^{mason.smith@unf.edu} ; James Gelsleichter ; Kelly J. Smith
• 关键词：Fish ; Hypoxia ; Biomarker ; Hypoxia-inducible factor ; HIF-1伪 ; Estuaries
• 刊名：Journal of Experimental Marine Biology and Ecology
• 出版年：2012
• 期刊代码：66_00220981
• 类别：abs
• 出版时间：1 August, 2012
• 卷：424-425
• 期：Complete
• 页码：1-4
• 文件大小：299 K

摘要

Fish kills often occur overnight from low dissolved oxygen (DO) events, however many mortality events are of unknown cause, since little water quality monitoring occurs overnight. The hypoxia-inducible factor 1伪 (HIF-1伪) protein is an excellent candidate as a biomarker for deciphering idiopathic fish kills. In this study, spot (Leiostomus xanthurus) were exposed to either constant or diel-cycling hypoxia, and HIF-1伪 expression was compared to normoxic control over three days. The results indicated that HIF-1伪 protein increased (p < 0.005) in muscle tissue after three days exposure to both constant and a simulated diel-cycling hypoxic event in a laboratory setting when compared to normoxic control animals. In comparison, diel-cycling hypoxia produced higher HIF-1伪 protein concentrations than constant hypoxia in all treatments, suggesting that the fluctuations of DO induced the expression of protein. The results from this study pose implications for using HIF-1伪 as a biomarker in wild populations, as overnight hypoxic cycles may produce higher protein concentrations than a constant hypoxic event, which would enable the detection of otherwise unnoticed hypoxic stress.