Umbilical cord mesenchymal stem cells suppress B-cell proliferation and differentiation

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• 作者：Nan Che^a ; ¹ ; Xia Li^a ; ¹ ; Shiliang Zhou^b ; Rui Liu^a ; Dongyan Shi^b ; Liwei Lu^c ; ^{liweilu@hkucc.hku.hk} ; Lingyun Sun^a ; ^{lingyunsun2001@yahoo.com.cn}
• 关键词：MSCs ; mesenchymal stem cells ; BM&ndash ; MSCs ; bone marrow&ndash ; mesenchymal stem cells ; UC&ndash ; MSCs ; umbilical cord-mesenchymal stem cells ; Blimp-1 ; B-lymphocyte-induced maturation protein-1 ; PAX-5 ; paired box gene-5 ; Xbp-1 ; X-box binding protein-1 ; Bcl-
• 刊名：Cellular Immunology
• 出版年：2012
• 期刊代码：279_00088749
• 类别：med
• 出版时间：2012
• 卷：274
• 期：1-2
• 页码：46-53
• 文件大小：772 K

摘要

Mesenchymal stem cells (MSCs) may be obtained from umbilical cord as an abundant and noninvasive source. However, the immunomodulatory properties of umbilical cord-MSCs (UC-MSCs) were poorly studied. In this study, we aimed to investigate the effects of UC-MSCs on B-cell proliferation and differentiation. UC-MSCs were found to suppress the proliferation of B cells isolated from murine spleen. Moreover, UC-MSCs markedly suppressed B-cell differentiation as shown by the decreased number of CD138 + cells and reduced levels of IgM and IgG production in coculture. As revealed by transwell experiments, soluble factors produced by UC-MSCs might be involved in mediating B-cell suppression. The Blimp-1 mRNA expression was suppressed whereas the PAX-5 mRNA expression was induced in coculture. Finally, UC-MSCs modified the phosphorylation pattern of Akt and p38 pathways, which were involved in B-cell proliferation and differentiation. These results may further support the potential therapeutic use of UC-MSCs in treating autoimmune disorders.