An ovine chitinase-like molecule, chitinase-3 like-1 (YKL-40), is upregulated in the abomasum in response to challenge with the gastrointestinal nematode, Teladorsagia circumcincta

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• 作者：P.A. Knight ; J. Pate ; W.D. Smith ; H.R.P. Miller
• 关键词：Teladorsagia ; Ovis aries ; Nematode ; Chitinase ; Chitinase-like protein
• 刊名：Veterinary Immunology and Immunopathology
• 出版年：2007
• 期刊代码：105_01652427
• 类别：abs
• 出版时间：15 November 2007
• 卷：120
• 期：1-2
• 页码：55-60
• 文件大小：214 K

摘要

Mammalian chitinases and chitinase-like proteins are a group of molecules known to be upregulated and secreted in Th2-induced inflammatory responses, such as asthma, allergy and nematode infection. As part of an investigation of potential components of the innate immune response to Teladorsagia circumcincta, a gastrointestinal nematode that colonises the abomasum in sheep, we carried out RT-PCR analysis of two members of the mammalian chitinase family of molecules, acidic chitinase (ChiA) and chitinase-3 like 1 (Chi3L1) using primers to homologous bovine/human sequences. Both sets of primers detected transcripts in the abomasum which were confirmed to be ovine ChiA and Chi3L1 by sequence analysis. Chi3L1 transcripts were found to be significantly upregulated in both the abomasum and gastric lymph nodes in response to T. circumcincta challenge of previously infected animals.