Hydrolysis of by-product adenosine diphosphate from 3-phosphoadenosine-5-phosphosulfate preparation using Nudix hydrolase NudJ

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• 作者：Feifei Bao ; Huihui Yan ; Hanju Sun ; Peizhou Yang…
• 关键词：PAPS ; ADP ; Nudix hydrolase ; NudJ ; Heparin
• 刊名：Applied Microbiology and Biotechnology
• 出版年：2015
• 出版时间：December 2015
• 年：2015
• 卷：99
• 期：24
• 页码：10771-10778
• 全文大小：655 KB
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• 作者单位：Feifei Bao (1) (2)
  Huihui Yan (1) (2)
  Hanju Sun (1) (2)
  Peizhou Yang (1) (2)
  Guoqing Liu (1) (2)
  Xianxuan Zhou (1) (2)
  
  1. School of Biotechnology and Food Engineering, Hefei University of Technology, Hefei, 230009, China
  2. Wanjiang Institute of Poultry Technology, Hefei University of Technology, Xuancheng Campus, Xuancheng, 242000, China
  
• 刊物类别：Chemistry and Materials Science
• 刊物主题：Chemistry
  Biotechnology
  Microbiology
  Microbial Genetics and Genomics
  
• 出版者：Springer Berlin / Heidelberg
• ISSN：1432-0614

文摘

3-Phosphoadenosine-5-phosphosulfate (PAPS) is the obligate cosubstrate and source of the sulfonate group in the chemoenzymatic synthesis of heparin, a clinically used anticoagulant drug. Previously, we have developed a method to synthesize PAPS with Escherichia coli crude extracts, which include three overexpressed enzymes and a fourth unidentified protein. The unknown protein degrades adenosine diphosphate (ADP), the by-product of PAPS synthesis reaction. To further understand and control the process of in vitro enzymatic PAPS synthesis, we decide to identify the fourth protein and develop a defined method to synthesize PAPS using purified enzymes. Here, we show that the purified Nudix hydrolase NudJ degrades ADP at high efficiency and serves as the fourth enzyme in PAPS synthesis. Under the defined condition of PAPS synthesis, all of the 10-mM ADP is hydrolyzed to form adenosine monophosphate (AMP) in a 15-min reaction. ADP is a better substrate for NudJ than adenosine triphosphate (ATP). Most importantly, the purified NudJ does not cleave the product PAPS. The removal of ADP makes the PAPS peak more separable from other components in the chromatographic purification process. This developed enzymatic approach of PAPS production will contribute to the chemoenzymatic synthesis of heparin. Keywords PAPS ADP Nudix hydrolase NudJ Heparin