Plasma microRNAs are promising novel biomarkers for the early detection of Toxoplasma gondii infection

详细信息    查看全文

• 作者：Boyin Jia (64)
  Zhiguang Chang (64)
  Xiaoyan Wei (64)
  Huijun Lu (64)
  Jigang Yin (64)
  Ning Jiang (64)
  Qijun Chen (64)
  
  64. Key Laboratory of Zoonosis ; Institute of Zoonosis/College of Veterinary Medicine ; Jilin University ; Xi An Da Lu 5333 ; Changchun ; 130062 ; China
  
• 关键词：Toxoplasma gondii ; Plasma ; MicroRNAs ; Biomarker ; Real ; time PCR
• 刊名：Parasites & Vectors
• 出版年：2014
• 出版时间：December 2014
• 年：2014
• 卷：7
• 期：1
• 全文大小：764 KB
• 参考文献：1. Dubey, J (1977) Toxoplasma, Hammondia, Besnotia, Sarcosystis, and Other Tissue Cyst-Forming Coccidia of Man and Animals. Academic Press, New York
  2. Dubey, J, Beattie, C (1988) Toxoplasmosis of Animals and Man. CRC Press, Boca Raton
  3. Sun, X, Lu, H, Jia, B, Chang, Z, Peng, S, Yin, J, Chen, Q, Jiang, N (2013) A comparative study of Toxoplasma gondii seroprevalence in three healthy Chinese populations detected using native and recombinant antigens. Parasit Vectors 6: pp. 241 CrossRef
  4. Fan, CK, Lee, LW, Liao, CW, Huang, YC, Lee, YL, Chang, YT, da Costa Ados, S, Gil, V, Chi, LH, Nara, T (2012) Toxoplasma gondii infection: relationship between seroprevalence and risk factors among primary schoolchildren in the capital areas of Democratic Republic of Sao Tome and Principe, West Africa. Parasit Vectors 5: pp. 141 CrossRef
  5. Sauer, A, de la Torre, A, Gomez-Marin, J, Bourcier, T, Garweg, J, Speeg-Schatz, C, Candolfi, E (2011) Prevention of retinochoroiditis in congenital toxoplasmosis: Europe versus South America. Pediatr Infect Dis J 30: pp. 601-603 CrossRef
  6. Bossi, P, Caumes, E, Astagneau, P, Li, TS, Paris, L, Mengual, X, Katlama, C, Bricaire, F (1998) Epidemiologic characteristics of cerebral toxoplasmosis in 399 HIV-infected patients followed between 1983 and 1994. Rev Med Interne 19: pp. 313-317 CrossRef
  7. Gao, XJ, Zhao, ZJ, He, ZH, Wang, T, Yang, TB, Chen, XG, Shen, JL, Wang, Y, Lv, FL, Hide, G, Lun, ZR (2012) Toxoplasma gondii infection in pregnant women in China. Parasitology 139: pp. 139-147 CrossRef
  8. Karczewski, G, Golab, E (2011) Diagnostic problems with congenital toxoplasmosis. Przegl Epidemiol 65: pp. 451-454
  9. Gilbert, R (2009) Treatment for congenital toxoplasmosis: finding out what works. Mem Inst Oswaldo Cruz 104: pp. 305-311 CrossRef
  10. Contini, C (2008) Clinical and diagnostic management of toxoplasmosis in the immunocompromised patient. Parassitologia 50: pp. 45-50
  11. Sensini, A (2006) Toxoplasma gondii infection in pregnancy: opportunities and pitfalls of serological diagnosis. Clin Microbiol Infect 12: pp. 504-512 CrossRef
  12. Lin, MH, Chen, TC, Kuo, TT, Tseng, CC, Tseng, CP (2000) Real-time PCR for quantitative detection of Toxoplasma gondii. J Clin Microbiol 38: pp. 4121-4125
  13. Toth, T, Sziller, I, Papp, Z (1998) PCR detection of Toxoplasma gondii in human fetal tissues. Methods Mol Biol 92: pp. 195-202
  14. Guy, EC, Pelloux, H, Lappalainen, M, Aspock, H, Hassl, A, Melby, KK, Holberg-Pettersen, M, Petersen, E, Simon, J, Ambroise-Thomas, P (1996) Interlaboratory comparison of polymerase chain reaction for the detection of Toxoplasma gondii DNA added to samples of amniotic fluid. Eur J Clin Microbiol Infect Dis 15: pp. 836-839 CrossRef
  15. Van Rooij, E, Marshall, WS, Olson, EN (2008) Toward microRNA-based therapeutics for heart disease: the sense in antisense. Circ Res 103: pp. 919-928 CrossRef
  16. Xu, MJ, Zhou, DH, Huang, SY, Zhao, FR, Nisbet, AJ, Lin, RQ, Song, HQ, Zhu, XQ (2013) Comparative characterization of microRNA profiles of different genotypes of Toxoplasma gondii. Parasitology 140: pp. 1111-1118 CrossRef
  17. Wang, J, Liu, X, Jia, B, Lu, H, Peng, S, Piao, X, Hou, N, Cai, P, Yin, J, Jiang, N, Chen, Q (2012) A comparative study of small RNAs in Toxoplasma gondii of distinct genotypes. Parasit Vectors 5: pp. 186 CrossRef
  18. Xu, MJ, Zhou, DH, Nisbet, AJ, Huang, SY, Fan, YF, Zhu, XQ (2013) Characterization of mouse brain microRNAs after infection with cyst-forming Toxoplasma gondii. Parasit Vectors 6: pp. 154 CrossRef
  19. Xiao, J, Li, Y, Prandovszky, E, Karuppagounder, SS, Talbot, CC, Dawson, VL, Dawson, TM, Yolken, RH (2014) MicroRNA-132 dysregulation in Toxoplasma gondii infection has implications for dopamine signaling pathway. Neuroscience 268: pp. 128-138 CrossRef
  20. Cai, Y, Chen, H, Jin, L, You, Y, Shen, J (2013) STAT3-dependent transactivation of miRNA genes following Toxoplasma gondii infection in macrophage. Parasit Vectors 6: pp. 356 CrossRef
  21. Mitchell, PS, Parkin, RK, Kroh, EM, Fritz, BR, Wyman, SK, Pogosova-Agadjanyan, EL, Peterson, A, Noteboom, J, O'Briant, KC, Allen, A, Lin, DW, Urban, N, Drescher, CW, Knudsen, BS, Stirewalt, DL, Gentleman, R, Vessella, RL, Nelson, PS, Martin, DB, Tewari, M (2008) Circulating microRNAs as stable blood-based markers for cancer detection. Proc Natl Acad Sci U S A 105: pp. 10513-10518 CrossRef
  22. Ng, EK, Chong, WW, Jin, H, Lam, EK, Shin, VY, Yu, J, Poon, TC, Ng, SS, Sung, JJ (2009) Differential expression of microRNAs in plasma of patients with colorectal cancer: a potential marker for colorectal cancer screening. Gut 58: pp. 1375-1381 CrossRef
  23. Silva, J, Garcia, V, Zaballos, A, Provencio, M, Lombardia, L, Almonacid, L, Garcia, JM, Dominguez, G, Pena, C, Diaz, R, Herrera, M, Varela, A, Bonilla, F (2011) Vesicle-related microRNAs in plasma of nonsmall cell lung cancer patients and correlation with survival. Eur Respir J 37: pp. 617-623 CrossRef
  24. Morimura, R, Komatsu, S, Ichikawa, D, Takeshita, H, Tsujiura, M, Nagata, H, Konishi, H, Shiozaki, A, Ikoma, H, Okamoto, K, Ochiai, T, Taniguchi, H, Otsuji, E (2011) Novel diagnostic value of circulating miR-18a in plasma of patients with pancreatic cancer. Br J Cancer 105: pp. 1733-1740 CrossRef
  25. Shen, J, Hruby, GW, McKiernan, JM, Gurvich, I, Lipsky, MJ, Benson, MC, Santella, RM (2012) Dysregulation of circulating microRNAs and prediction of aggressive prostate cancer. Prostate 72: pp. 1469-1477 CrossRef
  26. Caggana, M, Conroy, JM, Pass, KA (1998) Rapid, efficient method for multiplex amplification from filter paper. Hum Mutat 11: pp. 404-409 CrossRef
  27. Homan, WL, Vercammen, M, De Braekeleer, J, Verschueren, H (2000) Identification of a 200- to 300-fold repetitive 529 bp DNA fragment in Toxoplasma gondii, and its use for diagnostic and quantitative PCR. Int J Parasitol 30: pp. 69-75 CrossRef
  28. Ozer, J, Ratner, M, Shaw, M, Bailey, W, Schomaker, S (2008) The current state of serum biomarkers of hepatotoxicity. Toxicology 245: pp. 194-205 CrossRef
  29. Amacher, DE, Adler, R, Herath, A, Townsend, RR (2005) Use of proteomic methods to identify serum biomarkers associated with rat liver toxicity or hypertrophy. Clin Chem 51: pp. 1796-1803 CrossRef
  30. Fichtlscherer, S, De Rosa, S, Fox, H, Schwietz, T, Fischer, A, Liebetrau, C, Weber, M, Hamm, CW, Roxe, T, Muller-Ardogan, M, Bonauer, A, Zeiher, AM, Dimmeler, S (2010) Circulating microRNAs in patients with coronary artery disease. Circ Res 107: pp. 677-684 CrossRef
  31. Lawrie, CH, Gal, S, Dunlop, HM, Pushkaran, B, Liggins, AP, Pulford, K, Banham, AH, Pezzella, F, Boultwood, J, Wainscoat, JS, Hatton, CS, Harris, AL (2008) Detection of elevated levels of tumour-associated microRNAs in serum of patients with diffuse large B-cell lymphoma. Br J Haematol 141: pp. 672-675 CrossRef
  32. McDonald, JS, Milosevic, D, Reddi, HV, Grebe, SK, Algeciras-Schimnich, A (2011) Analysis of circulating microRNA: preanalytical and analytical challenges. Clin Chem 57: pp. 833-840 CrossRef
  33. Son, DJ, Kumar, S, Takabe, W, Kim, CW, Ni, CW, Alberts-Grill, N, Jang, IH, Kim, S, Kim, W, Won Kang, S, Baker, AH, Woong Seo, J, Ferrara, KW, Jo, H (2013) The atypical mechanosensitive microRNA-712 derived from pre-ribosomal RNA induces endothelial inflammation and atherosclerosis. Nat Commun 4: pp. 3000 CrossRef
  34. Gui, T, Zhou, G, Sun, Y, Shimokado, A, Itoh, S, Oikawa, K, Muragaki, Y (2012) MicroRNAs that target Ca (2+) transporters are involved in vascular smooth muscle cell calcification. Lab Invest 92: pp. 1250-1259 CrossRef
  35. Yu, L, Liao, Q, Zeng, X, Lv, Z, Zheng, H, Zhao, Y, Sun, X, Wu, Z (2014) MicroRNA expressions associated with eosinophilic meningitis caused by Angiostrongylus cantonensis infection in a mouse model. Eur J Clin Microbiol Infect Dis 33: pp. 1457-1465 CrossRef
  36. Tserel, L, Runnel, T, Kisand, K, Pihlap, M, Bakhoff, L, Kolde, R, Peterson, H, Vilo, J, Peterson, P, Rebane, A (2011) MicroRNA expression profiles of human blood monocyte-derived dendritic cells and macrophages reveal miR-511 as putative positive regulator of Toll-like receptor 4. J Biol Chem 286: pp. 26487-26495 CrossRef
  37. Hou, B, Benson, A, Kuzmich, L, DeFranco, AL, Yarovinsky, F (2011) Critical coordination of innate immune defense against Toxoplasma gondii by dendritic cells responding via their Toll-like receptors. Proc Natl Acad Sci U S A 108: pp. 278-283 CrossRef
  38. Li, H, Zhao, J, Zhang, JW, Huang, QY, Huang, JZ, Chi, LS, Tang, HJ, Liu, GQ, Zhu, DJ, Ma, WM (2013) MicroRNA-217, down-regulated in clear cell renal cell carcinoma and associated with lower survival, suppresses cell proliferation and migration. Neoplasma 60: pp. 511-515 CrossRef
  39. Zhao, WG, Yu, SN, Lu, ZH, Ma, YH, Gu, YM, Chen, J (2010) The miR-217 microRNA functions as a potential tumor suppressor in pancreatic ductal adenocarcinoma by targeting KRAS. Carcinogenesis 31: pp. 1726-1733 CrossRef
  
• 刊物主题：Parasitology; Infectious Diseases; Tropical Medicine; Entomology;
• 出版者：BioMed Central
• ISSN：1756-3305

文摘

Background MicroRNAs (miRNAs) have been shown to be present in plasma, which are remarkably stable, and have been suggested as disease biomarkers. Toxoplasma gondii (T. gondii) is a protozoan parasite that is infective to a wide range of animals and human beings. Previous studies have found that the parasite generated a large number of miRNAs during proliferation and it is known that the spectrum of miRNA expression in the infected hosts is pathogen-specific. To date, there are no reports regarding the application of microRNAs as biomarkers for the early detection of T. gondii infection. Methods In this study, we investigated the expression patterns of 414 murine miRNAs and tested their expression levels in the plasma after T. gondii infection by real-time PCR, with an ultimate purpose of identifying infection-related miRNAs. Three miRNAs in particular, exhibiting prominently elevated expressions, were further validated in a large number of infected mice. The Toxoplasma infection-specific miRNAs were confirmed by comparing their expression levels with those of mice infected with Plasmodium berghei, P. yoelii, P. chabaudi, Cryptosporidium parvum, Mouse hepatitis virus, and Staphylococcus aureus. Results Among the 414 miRNA candidates identified by a real-time PCR array, 71 were found to be up-regulated in the plasma of T. gondii infected mice. Three of those miRNAs (mmu-miR-712-3p, mmu-miR-511-5p and mmu-miR-217-5p) were prominently expressed in mice infected by both the RH and ME49 strains of T. gondii. Additionally, the elevated expression of these miRNAs was Toxoplasma-specific. Conclusions The levels of the three miRNAs, mmu-miR-712-3p, mmu-miR-511-5p and mmu-miR-217-5p miRNAs, were found specifically up-regulated in plasma of mice after T. gondii infection.