Heparan sulfate 6-O-sulfotransferase 3 is involved in bone marrow mesenchymal stromal cell osteogenic differentiation

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• 作者：Shancheng Zhao (1)
  Chao Deng (2)
  Zhen Wang (1)
  Liping Teng (2)
  Jinghua Chen (1) (3)
  
  1. School of Pharmaceutical Science ; Jiangnan University ; Wuxi ; 214122 ; PR China
  2. Wuxi Medical School ; Jiangnan University ; Wuxi ; 214122 ; PR China
  3. Key Laboratory of Carbohydrate Chemistry and Biotechnology ; Ministry of Education ; Jiangnan University ; Wuxi ; 214122 ; PR China
  
• 关键词：BMMSCs ; osteogenic differentiation ; extracellular matrix ; heparan sulfate ; sulfotransferase
• 刊名：Biochemistry (Moscow)
• 出版年：2015
• 出版时间：March 2015
• 年：2015
• 卷：80
• 期：3
• 页码：379-389
• 全文大小：1,026 KB
• 参考文献：1. David, W (2012) The National Academies Collection: reports funded by National Institutes of Health. Transforming Glycoscience: A Roadmap for the Future. National Academies Press (US), National Academy of Sciences, Washington (DC)
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• 刊物类别：Biomedical and Life Sciences
• 刊物主题：Life Sciences
  Biochemistry
  Bioorganic Chemistry
  Microbiology
  Biomedicine
  Russian Library of Science
  
• 出版者：MAIK Nauka/Interperiodica distributed exclusively by Springer Science+Business Media LLC.
• ISSN：1608-3040

文摘

The roles of sugar chains such as heparan sulfate (HS) in stem cell self-renewal and differentiation are poorly understood. HS is a sugar chain with linear sulfated polyanionic disaccharide repeating structures that interact with many proteins, including structural proteins in the extracellular matrix and growth factors and their receptors. Thus, unraveling the role of HS in stem cell self-renewal and differentiation could provide new insights and technical routes in clinical stem cell applications. Here, we purified rat bone marrow mesenchymal stromal cells (BMMSCs) by density gradient centrifugation, analyzed mesenchymal stromal cell surface stemness marker expression by flow cytometry, and identified the sulfotransferases responsible for sulfation ester modification of HS. An osteogenic differentiation model was established by chemical induction reagents and confirmed via alkaline phosphatase (ALP) activity detection and the expression of the osteogenic differentiation markers Runx2 and Ocn. The expression profiles of HS sulfotransferases in rat BMMSCs before and after osteogenic induction were detected by RT-PCR and Western blot. Cell spheroids were formed in both control and osteogenic culture systems when BMMSCs were grown to high confluence. We determined that this type of cell spheroid was a highly calcified nodule by histochemical staining. Among all the sulfotransferases examined, heparan sulfate 6-O-sulfotransferase 3 (HS6ST3) mRNA and protein were upregulated in these calcified cell spheroids. HS6ST3 knockdown BMMSCs were established with RNA interference, and they had significantly lower ALP activity and decreased expression of the osteogenic differentiation markers Runx2 and Ocn. These findings suggest that HS6ST3 is involved in BMMSC differentiation, and new glycotherapeutic-based technologies could be developed in the future.