Monitoring morphological alterations during invasive ductal breast carcinoma progression using multiphoton microscopy

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• 作者：Yan Wu (1) (4)
  Fangmeng Fu (2)
  Yuane Lian (3)
  Jianxin Chen (1)
  Chuan Wang (2)
  Yuting Nie (1)
  Liqin Zheng (1)
  Shuangmu Zhuo (1)
  
  1. Institute of Laser and Optoelectronics Technology ; Fujian Provincial Key Laboratory for Photonics Technology ; Key Laboratory of OptoElectronic Science and Technology for Medicine of Ministry of Education ; Fujian Normal University ; Fuzhou ; 350007 ; China
  4. School of Science ; Jimei University ; Xiamen ; 361021 ; China
  2. Department of Breast Surgery ; The Affiliated Union Hospital of Fujian Medical University ; Fuzhou ; 350001 ; China
  3. Department of Pathology ; The Affiliated Union Hospital of Fujian Medical University ; Fuzhou ; 350001 ; China
  
• 关键词：Multiphoton microscopy (MPM) ; Invasive ductal breast carcinoma (IDC) ; Morphological alterations ; Two ; photon excited fluorescence (TPEF) ; Second harmonic generation (SHG)
• 刊名：Lasers in Medical Science
• 出版年：2015
• 出版时间：April 2015
• 年：2015
• 卷：30
• 期：3
• 页码：1109-1115
• 全文大小：1,360 KB
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• 刊物类别：Medicine
• 刊物主题：Medicine & Public Health
  Medicine/Public Health, general
  Dentistry
  Laser Technology and Physics and Photonics
  Quantum Optics, Quantum Electronics and Nonlinear Optics
  Applied Optics, Optoelectronics and Optical Devices
  
• 出版者：Springer London
• ISSN：1435-604X

文摘

Morphological alteration of cells and matrices is critical for tumor initiation and progression. Monitoring these alterations during tumor progression is vitally important for making real-time histological diagnoses of tumor staging. In this study, 20 pairs of normal and cancerous human breast tissues were imaged by multiphoton microscopy (MPM), and nuclear area and collagen density were quantified by LSM 5 software (version 3.2). Comparison of MPM images from normal breast tissue with low- and high-grade invasive ductal carcinoma (IDC) lesions clearly showed changes in both cellular features and extracellular matrix architecture during IDC development. Moreover, analysis of nuclear area and collagen density established a quantitative link between these two morphological features and progression of IDC. Present results demonstrated that MPM can provide both qualitative and quantitative evaluations of tumor progression. With additional development, this technique has the potential to make real-time histological diagnoses of tumor staging and guide development of efficacious clinical therapies.