Development and characterization of genomic microsatellite markers in Apterosperma oblata (Theaceae) and cross-amplification in Euryodendron excelsum (Ternstroemiaceae)

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• 作者：Zhen Wang (1)
  Zhengyong Hu (1)
  Lu Huang (2)
  
  1. Guangdong Experimental High School ; Guangzhou ; 510375 ; China
  2. School of Life Sciences ; Sun Yat-sen University ; Guangzhou ; 510275 ; China
  
• 关键词：Apterosperma oblata ; FIASCO ; Adaptive evolution ; Cross ; amplification
• 刊名：Conservation Genetics Resources
• 出版年：2015
• 出版时间：March 2015
• 年：2015
• 卷：7
• 期：1
• 页码：119-120
• 全文大小：150 KB
• 参考文献：1. IUCN (2014) IUCN red list of threatened species, version 2014.1. www.iucnredlist.org
  2. Su YJ, Liao WB, Wang T, Sun YF, Wei Q, Chang HT (2011) Phylogeny and evolutionary divergence times in / Apterosperma and / Euryodendron: evidence of a Tertiary origin in south China. Biochem Syst Ecol 39:769鈥?77 CrossRef
  3. Su YJ, Zhan QJ, Wang T, Ying ZM, Ye HG (2008) High ISSR variation in 24 surviving individuals of / Apterosperma oblata (Theaceae) endemic to China. Biochem Syst Ecol 36:619鈥?25 CrossRef
  4. Su YJ, Wang T, Sun YF, Ye HG (2009) High ISSR variation in 14 surviving individuals of / Euryodendron excelsum (Ternstroemiaceae) endemic to China. Biochem Genet 47:56鈥?5 CrossRef
  5. Zane L, Bargelloni L, Patarnello T (2002) Strategies for microsatellite isolation: a review. Mol Ecol 11:1鈥?6 CrossRef
  
• 刊物主题：Conservation Biology/Ecology; Plant Sciences; Animal Anatomy / Morphology / Histology; Evolutionary Biology;
• 出版者：Springer Netherlands
• ISSN：1877-7260

文摘

Apterosperma oblata is a vulnerable species endemic to south China with highly narrow distribution. We have developed 12 polymorphic microsatellite markers using FIASCO protocol. These loci produced alleles ranging from two to seven, and expected heterozygosity from 0.105 to 0.773. Among them, seven loci were successfully cross-amplified in Euryodendron excelsum, of which three loci exhibited polymorphism. These markers will promote further investigation of adaptive evolution in A. oblata and E. excelsum, and be useful for establishing conservation strategies.