Electrospinning of PELA/PPY Fibrous Conduits: Promoting Peripheral Nerve Regeneration in Rats by Self-Originated Electrical Stimulation

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• 作者：Zi-Fei Zhou ; Fan Zhang ; Jian-Guang Wang ; Quan-Chi Chen ; Wei-Zhi Yang ; Ning He ; Ying-Ying Jiang ; Feng Chen ; Jun-Jian Liu
• 刊名：ACS Biomaterials Science & Engineering
• 出版年：2016
• 出版时间：September 12, 2016
• 年：2016
• 卷：2
• 期：9
• 页码：1572-1581
• 全文大小：790K
• ISSN：2373-9878

文摘

Peripheral nerve injuries represent a great challenge for surgeons. The conductive neural scaffold has experienced increasing interest because of its good biocompatibility and similar electrical properties as compared to those of a normal nerve. Herein, nerve conduits made from poly(d,l-lactide)-co-poly(ethylene glycol) and polypyrrole (20%, 30%, and 50%) (PELA–PPY) were prepared by electrospinning, and used in regeneration of peripheral nerve defects. The results of an in vitro experiment indicated a high biocompatibility for the as-prepared materials, supporting the attachment and proliferation of a rat pheochromocytoma PC-12 cell. Furthermore, the PELA–PPY nerve conduit implanted in the sciatic nerve defects (10 mm) of the Spraguee–Dawley rats for 12 weeks showed similar results with the autograft, while it demonstrated a better outcome than the PELA nerve conduit in electrophysiological examination, sciatic function index, total amount of regenerated myelinated nerve fibers, axon diameter, myelin thickness, and several immunohistochemistry indices (S-100, laminin, neurofilament, bromodeoxyuridine, and glial fibrillary acidic portein). We supposed that the bioactivity is mainly generated by the PPY in composite nanofibers which could transmit self-originated electrical stimulation between cells. Due to the facile preparation and excellent in vivo performance, the PPY–PELA nerve conduit is promising for use as a bioengineered biomaterial for peripheral nerve regeneration.