Fluorescent Nanocomposite for Visualizing Cross-Talk between MicroRNA-21 and Hydrogen Peroxide in Ischemia-Reperfusion Injury in Live Cells and In Vivo

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• 作者：Limin Yang ; Yanfei Ren ; Wei Pan ; Zhengze Yu ; Lili Tong ; Na Li ; Bo Tang
• 刊名：Analytical Chemistry
• 出版年：2016
• 出版时间：December 6, 2016
• 年：2016
• 卷：88
• 期：23
• 页码：11886-11891
• 全文大小：352K
• ISSN：1520-6882

文摘

MicroRNAs (miRNAs) and reactive oxygen species (ROS) are concurrently implicated in heart ischemia-reperfusion (IR) injury. There may exist mutual cross-talk between miRNAs and ROS in cardiac IR injury process. In this study, we developed a novel crown-like silica@polydopamine-DNA-CeO₂ nanocomposite by assembly of silica@polydopamine-DNA1 nanoparticles decorated with satellite CeO₂-DNA2 nanoparticles for detecting and imaging of microRNA-21 (miR-21) and hydrogen peroxide (H₂O₂) in simulated IR injury in living cells and in vivo. The miRNA-21 was found to be regulated by H₂O₂ via PI3K/AKT signaling pathway for the first time in H9C2 cells in simulated ischemia-reperfusion injury. H₂O₂ and miRNA-21 are overproduced during mimicked heart ischemia-reperfusion injury, suggesting that they are closely related to reperfusion injury. All these results reveal that there is definite cross-talk between miR-21 and H₂O₂ in IR injury. The current method can provide a promising strategy to further explore the interplaying roles between ROS and miRNAs in other pathological processes.