Environment-Sensitive Fluorescent Probe for the Human Ether-a-go-go-Related Gene Potassium Channel

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• 作者：Zhenzhen Liu ; Tianyu Jiang ; Beilei Wang ; Bowen Ke ; Yubin Zhou ; Lupei Du ; Minyong Li
• 刊名：Analytical Chemistry
• 出版年：2016
• 出版时间：February 2, 2016
• 年：2016
• 卷：88
• 期：3
• 页码：1511-1515
• 全文大小：310K
• ISSN：1520-6882

文摘

A novel environment-sensitive probe S2 with turn-on switch for Human Ether-a-go-go-Related Gene (hERG) potassium channel was developed herein. After careful evaluation, this fluorescent probe showed high binding affinity with hERG potassium channel with an IC₅₀ value of 41.65 nM and can be well applied to hERG channel imaging or cellular distribution study for hERG channel blockers. Compared with other imaging techniques, such as immunofluorescence and fluorescent protein-based approaches, this method is convenient and affordable, especially since a washing procedure is not needed. Meanwhile, this environment-sensitive turn-on design strategy may provide a good example for the probe development for these targets that have no reactive or catalytic activity.