Covalent Linkage of One-Dimensional DNA Arrays Bonded by Paranemic Cohesion

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• 作者：Yoel P. Ohayon ; Ruojie Sha ; Ortho Flint ; Wenyan Liu ; Banani Chakraborty ; Hari K. K. Subramanian ; Jianping Zheng ; Arun Richard Chandrasekaran ; Hatem O. Abdallah ; Xing Wang ; Xiaoping Zhang ; Nadrian C. Seeman
• 刊名：ACS Nano
• 出版年：2015
• 出版时间：October 27, 2015
• 年：2015
• 卷：9
• 期：10
• 页码：10304-10312
• 全文大小：773K
• ISSN：1936-086X

文摘

The construction of DNA nanostructures from branched DNA motifs, or tiles, typically relies on the use of sticky-ended cohesion, owing to the specificity and programmability of DNA sequences. The stability of such constructs when unligated is restricted to a specific range of temperatures, owing to the disruption of base pairing at elevated temperatures. Paranemic (PX) cohesion was developed as an alternative to sticky ends for the cohesion of large topologically closed species that could be purified reliably on denaturing gels. However, PX cohesion is also of limited stability. In this work, we added sticky-ended interactions to PX-cohesive complexes to create interlocked complexes by functionalizing the sticky ends with psoralen, which can form cross-links between the two strands of a double helix. We were able to reinforce the stability of the constructs by creating covalent linkages between the 3鈥?ends and 5鈥?ends of the sticky ends; the sticky ends were added to double crossover domains via 3鈥测€?鈥?and 5鈥测€?鈥?linkages. Catenated arrays were obtained either by enzymatic ligation or by UV cross-linking. We have constructed finite-length one-dimensional arrays linked by interlocking loops and have positioned streptavidin鈥揼old particles on these constructs.