Plasmonic Imaging of Brownian Motion of Single DNA Molecules Spontaneously Binding to Ag Nanoparticles

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• 作者：Ken Hirano ; Tomomi Ishido ; Yuko S. Yamamoto ; Norio Murase ; Masatoshi Ichikawa ; Kenichi Yoshikawa ; Yoshinobu Baba ; Tamitake Itoh
• 刊名：Nano Letters
• 出版年：2013
• 出版时间：May 8, 2013
• 年：2013
• 卷：13
• 期：5
• 页码：1877-1882
• 全文大小：364K
• 年卷期：v.13,no.5(May 8, 2013)
• ISSN：1530-6992

文摘

We find the spontaneous binding of single DNA molecules to uncoated silver nanoparticles (AgNPs) in aqueous solution with Mn²⁺ (3 mM). From dark-field optical microscopic imaging of AgNPs bound to DNA molecules, we demonstrate analysis of the Brownian motion of single DNA molecules via plasmon resonance elastic light scattering. Our results provide that the plasmonic imaging technique is free from photobleaching and blinking and thus is useful in long-time observations of single-molecule DNA dynamics.

Keywords:

Silver nanoparticle; plasmon resonance; single DNA molecule; DNA labeling; video-rate imaging; nanoparticle tracking