Polyphenolics Composition of the Leaves of Zanthoxylum bungeanum Maxim. Grown in Hebei, China, and Their Radical Scavenging Activities

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• 作者：Li-Chen Yang ; Rong Li ; Jin Tan ; Zi-Tao Jiang
• 刊名：Journal of Agricultural and Food Chemistry
• 出版年：2013
• 出版时间：February 27, 2013
• 年：2013
• 卷：61
• 期：8
• 页码：1772-1778
• 全文大小：301K
• 年卷期：v.61,no.8(February 27, 2013)
• ISSN：1520-5118

文摘

High performance liquid chromatography coupled with tandem mass spectrometry with electrospray ionization and negative ion detection (HPLC-ESI-MS/MS) was used to identify the polyphenols from the leaves of a traditional vegetable and spice, Zanthoxylum bungeanum Maxim., in China by comparison of the retention times and mass spectral fragment with standard substances or related literature. A phenolic acid glucoside, two acids, and eight flavonoids were determined including vanillic acid-4-glucoside (22.75 渭g/g, dry weight of leaves), quinic acid (58.58 渭g/g), chlorogenic acid (2515.96 渭g/g), epicatechin (77.80 渭g/g), 5-feruloyquinic acid (16.63 渭g/g), syringetin-3-glucoside (103.23 渭g/g), rutin (89.41 渭g/g), hyperoside (886.36 渭g/g), quercetin-3-arabinoside (118.75 渭g/g), quercitrin (645.82 渭g/g), and isorhamnetin-3-glucoside (104.27 渭g/g). The obtained polyphenols show good radical scavenging activities in DPPH and superoxide anion. Through the intracellular antioxidation assay, the result shows that the polyphenols could reduce the ROS in HT-29 cells without cell toxicity.

Keywords:

Zanthoxylum bungeanum Maxim.; leaves; polyphenols; HPLC-ESI-MS/MS; radical scavenging activity; intracellular antioxidation