Surface Preparation Strategies for Improved Parallelization and Reproducible MALDI-TOF MS Ligand Binding Assays

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• 作者：Michael J. Roth ; Erica M. Maresh ; Daniel A. Plymire ; Junmei Zhang ; John R. Corbett ; Roger Robbins ; Steven M. Patrie
• 刊名：ACS Applied Materials & Interfaces
• 出版年：2013
• 出版时间：January 9, 2013
• 年：2013
• 卷：5
• 期：1
• 页码：6-10
• 全文大小：326K
• 年卷期：v.5,no.1(January 9, 2013)
• ISSN：1944-8252

文摘

Immunoassays are employed in academia and the healthcare and biotech industries for high-throughput, quantitative screens of biomolecules. We have developed monolayer-based immunoassays for MALDI-TOF MS. To improve parallelization, we adapted the workflow to photolithography-generated arrays. Our work shows Parylene-C coatings provide excellent 鈥渟olvent pinning鈥?for reagents and biofluids, enabling sensitive MS detection of immobilized components. With a unique MALDI-matrix crystallization technique we show routine interassay RSD <10% at picomolar concentrations and highlight platform compatibility for relative and label-free quantitation applications. Parylene-arrays provide high sample densities and promise screening throughputs exceeding 1000 samples/h with modern liquid-handlers and MALDI-TOF systems.

Keywords:

mass spectrometry; self-assembled monolayers; MALDI-TOF; arrays; immunoassays; biomarker