Determination of Estrogens in Pork and Chicken Samples by Stir Bar Sorptive Extraction Combined with High-Performance Liquid Chromatography鈥揢ltraviolet Detection

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• 作者：Cong Hu ; Man He ; Beibei Chen ; Bin Hu
• 刊名：Journal of Agricultural and Food Chemistry
• 出版年：2012
• 出版时间：October 24, 2012
• 年：2012
• 卷：60
• 期：42
• 页码：10494-10500
• 全文大小：272K
• 年卷期：v.60,no.42(October 24, 2012)
• ISSN：1520-5118

文摘

A poly(dimethylsiloxane) (PDMS)/尾-cyclodextrin (尾-CD)/divinylbenzene (DVB)-coated stir bar was prepared by the sol鈥揼el technique for the stir bar sorptive extraction (SBSE) of four estrogens from animal-derived foods, followed by liquid desorption (LD) and high-performance liquid chromatography鈥搖ltraviolet (HPLC鈥揢V) detection. The influence of the coating composition on SBSE of target estrogens was investigated by an orthogonal experiment design, and the prepared PDMS/尾-CD/DVB-coated stir bars show good reproducibility. Under the optimal experimental conditions, the limits of detection (S/N = 3) of the developed PDMS/尾-CD/DVB SBSE鈥揕D鈥揌PLC鈥揢V method were 0.21鈥?.6 渭g/L for the target estrogens with enrichment factors of 19鈥?1-fold, the dynamic linear range was 2鈥?000 渭g/L, and the relative standard deviations of the method ranged from 6.0% to 9.7% (n = 8, c = 100 渭g/L) and from 8.4% to 11.7% (n = 8, c = 10 渭g/L). The developed method was simple, sensitive, and selective and was successfully applied to the analysis of estrogens in pork and chicken samples.

Keywords:

stir bar sorptive extraction; poly(dimethylsiloxane)/尾-cyclodextrin/divinylbenzene coating; estrogens; mance+liquid+chromatography&qsSearchArea=searchText">high-performance liquid chromatography; pork and chicken