Assembly of MreB Filaments on Liposome Membranes: A Synthetic Biology Approach

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• 作者：Yusuke T. Maeda ; Tomoyoshi Nakadai ; Jonghyeon Shin ; Kunihiro Uryu ; Vincent Noireaux ; Albert Libchaber
• 刊名：ACS Synthetic Biology
• 出版年：2012
• 出版时间：February 17, 2012
• 年：2012
• 卷：1
• 期：2
• 页码：53-59
• 全文大小：318K
• 年卷期：v.1,no.2(February 17, 2012)
• ISSN：2161-5063

文摘

The physical interaction between the cytoskeleton and the cell membrane is essential in defining the morphology of living organisms. In this study, we use a synthetic approach to polymerize bacterial MreB filaments inside phospholipid vesicles. When the proteins MreB and MreC are expressed inside the liposomes, the MreB cytoskeleton structure develops at the inner membrane. Furthermore, when purified MreB is used inside the liposomes, MreB filaments form a 4鈥?0 渭m rigid bundle structure and deform the lipid vesicles in physical contact with the vesicle inner membrane. These results indicate that the fibrillation of MreB filaments can take place either in close proximity of deformable lipid membrane or in the presence of associated protein. Our finding might be relevant for the self-assembly of cytoskeleton filaments toward the construction of artificial cell systems.

Keywords:

bacterial cytoskeleton MreB; cell-free gene expression; vesicle bioreactor; artificial cells