Potentiometric Immunosensor Using Artificial Antibody Based on Molecularly Imprinted Polymers

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• 作者：Tatsuya Kitade ; Keisuke Kitamura ; Tomoyuki Konishi ; Shigehiko Takegami ; Takayoshi Okuno ; Michie Ishikawa ; Manabu Wakabayashi ; Kayoko Nishikawa ; and Yoko Muramatsu
• 刊名：Analytical Chemistry
• 出版年：2004
• 出版时间：November 15, 2004
• 年：2004
• 卷：76
• 期：22
• 页码：6802 - 6807
• 全文大小：418K
• 年卷期：v.76,no.22(November 15, 2004)
• ISSN：1520-6882

文摘

A potentiometric artificial immunosensor based on amolecularly imprinted polymer was prepared as a detecting element in micro total analysis systems with the intentof providing easy clinical analysis. As the structure andtransducing mechanism of this sensor are very simple,construction of a single microsensor should be quite easy.Multimicrosensor arrays applicable to several kinds ofanalytes will be attainable by both changing the templatemolecule to be imprinted and reducing the sensor size.The response characteristics of this sensor were evaluatedby measuring the response potential to serotonin, whichwas used as a model material. The obtained sensor washighly responsive to serotonin in water but not totryptamine, acetaminophen, or procainamide. This phenomenon confirms that the sensor recognizes serotoninand that it functions as a specific artificial immunosensor.Quick measurement is possible because the responsetime, defined as the time required to achieve 95% of themagnitude of the equilibrated signal, correspond to ~12s. The sensor's determination and detection limits werefound to be 1 mol/L and 100 pmol/L, respectively.These results suggest that our strategy can be applied toconstruction of a potentiometric artificial immunosensor.