The Proteomes of Human Parotid and Submandibular/Sublingual Gland Salivas Collected as the Ductal Secretions

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• 作者：Paul Denny ; Fred K. Hagen ; Markus Hardt ; Lujian Liao ; Weihong Yan ; Martha Arellanno ; Sara Bassilian ; Gurrinder S. Bedi ; Pinmannee Boontheung ; Daniel Cociorva ; Claire M. Delahunty ; Trish Denny ; Jason D
• 刊名：Journal of Proteome Research
• 出版年：2008
• 出版时间：May 2008
• 年：2008
• 卷：7
• 期：5
• 页码：1994 - 2006
• 全文大小：5591K
• 年卷期：v.7,no.5(May 2008)
• ISSN：1535-3907

文摘

Saliva is a body fluid with important functions in oral and general health. A consortium of three research groups catalogued the proteins in human saliva collected as the ductal secretions: 1166 identifications914 in parotid and 917 in submandibular/sublingual salivawere made. The results showed that a high proportion of proteins that are found in plasma and/or tears are also present in saliva along with unique components. The proteins identified are involved in numerous molecular processes ranging from structural functions to enzymatic/catalytic activities. As expected, the majority mapped to the extracellular and secretory compartments. An immunoblot approach was used to validate the presence in saliva of a subset of the proteins identified by mass spectrometric approaches. These experiments focused on novel constituents and proteins for which the peptide evidence was relatively weak. Ultimately, information derived from the work reported here and related published studies can be used to translate blood-based clinical laboratory tests into a format that utilizes saliva. Additionally, a catalogue of the salivary proteome of healthy individuals allows future analyses of salivary samples from individuals with oral and systemic diseases, with the goal of identifying biomarkers with diagnostic and/or prognostic value for these conditions; another possibility is the discovery of therapeutic targets.