Caenorhabditis elegans Bicarbonate Transporter ABTS-1 Is Involved in Arsenite Toxicity and Cholinergic Signaling

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• 作者：Vivian Hsiu-Chuan Liao ; Jui-Tung Liu ; Wen-Hsuan Li ; Chan-Wei Yu ; Yi-Chen Hsieh
• 刊名：Chemical Research in Toxicology
• 出版年：2010
• 出版时间：May 17, 2010
• 年：2010
• 卷：23
• 期：5
• 页码：926-932
• 全文大小：189K
• 年卷期：v.23,no.5(May 17, 2010)
• ISSN：1520-5010

文摘

Arsenic poisoning affects millions of people worldwide. Although there is accumulating evidence to suggest that the nervous system is a target of arsenic, relatively little information is known regarding its effects on the nervous system. The effects of arsenite on the nervous system in Caenorhabditis elegans were investigated in the present study. We found that abts-1, which encodes a Na⁺-dependent Cl⁻/HCO₃⁻ transporter, is required to protect C. elegans from arsenite toxicity. The abts-1::GFP transgene is primarily expressed in neurons and the hypodermis, but stronger expression was also observed in the pharynx and body wall muscle cells after exposure to arsenite. The steady-state level of ABTS-1 mRNA increased in response to arsenite exposure. We showed that worms lacking abts-1 are hypersensitive to the paralytic effects of the cholinesterase inhibitor, aldicarb, and the nicotinic acetylcholine receptor agonist, levamisole. We also showed that arsenite enhanced sensitivity to aldicarb and levamisole in abts-1 mutant worms. Our results indicate neuronal effects of arsenite and the ABTS-1 bicarbonate transporter.