Osthole Regulates Inflammatory Mediator Expression through Modulating NF-κB, Mitogen-Activated Protein Kinases, Protein Kinase C, and Reactive Oxygen Species

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• 作者：Pei-Chun Liao ; Shih-Chang Chien ; Chen-Lung Ho ; Eugene I-Chen Wang ; Shu-Ching Lee ; Yueh-Hsiung Kuo ; Narumon Jeyashoke ; Jie Chen ; Wei-Chih Dong ; Louis Kuoping Chao ; Kuo-Feng Hua
• 刊名：Journal of Agricultural and Food Chemistry
• 出版年：2010
• 出版时间：October 13, 2010
• 年：2010
• 卷：58
• 期：19
• 页码：10445-10451
• 全文大小：874K
• 年卷期：v.58,no.19(October 13, 2010)
• ISSN：1520-5118

文摘

Osthole, a coumarin compound, has been reported to exhibit various biological activities; however the cellular mechanism of its immune modulating activity has not yet been fully addressed. In this study we isolated osthole from the seeds of Cnidium monnieri and demonstrated that osthole inhibited TNF-α, NO and COX-2 expression in LPS-stimulated macrophages, without reducing the expression of IL-6. Furthermore, the phosphorylation of p38, JNK1/2, PKC-α and PKC-ε induced by LPS was inhibited by osthole; however, the phosphorylation of ERK1/2 and PKC-δ was not reduced by osthole. Osthole also inhibited NF-κB activation and ROS release in LPS-stimulated macrophages. Our current results indicated that osthole is the major anti-inflammatory ingredient of Cnidium monnieri seed ethanol extract.

Keywords:

Osthole; LPS; inflammation; signaling