Immobilization of Trametes versicolor Laccase on Magnetically Separable Mesoporous Silica Spheres

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• 作者：Yufang Zhu ; Stefan Kaskel ; Jianlin Shi ; Tobias Wage ; Karl-Heinz van Pé ; e
• 刊名：Chemistry of Materials
• 出版年：2007
• 出版时间：December 25, 2007
• 年：2007
• 卷：19
• 期：26
• 页码：6408 - 6413
• 全文大小：920K
• 年卷期：v.19,no.26(December 25, 2007)
• ISSN：1520-5002

文摘

Magnetic mesoporous silica spheres (MSS) with large pore size were prepared by reducing Fe³⁺-containing mesoporous silica spheres. Laccase from Trametes versicolor was immobilized on magnetic mesoporous silica spheres through physical adsorption and covalent attachment methods. Laccase oxidizes 2,2′-azinobis(3-ethylbenzthiazolin-6-sulfonate) (ABTS) to the green cation radical (ABTS^•+) as a model system; the immobilized laccase retained the activity and exhibited higher resistance to pH changes and thermal stability. The immobilized laccase obtained through covalent attachment almost has no leaching and can retain above 70% of activity after 10 consecutive operations. More interesting, the immobilized laccase can be separated quickly using an external magnetic field. Therefore, the magnetite-containing mesoporous silica spheres are a promising support for enzyme immobilization.