Application of Quantum Dot-Antibody Conjugates for Detection of Sulfamethazine Residue in Chicken Muscle Tissue

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• 作者：Shuangyang Ding ; Junxia Chen ; Haiyang Jiang ; Jihong He ; Weimin Shi ; Wenshen Zhao ; Jianzhong Shen
• 刊名：Journal of Agricultural and Food Chemistry
• 出版年：2006
• 出版时间：August 23, 2006
• 年：2006
• 卷：54
• 期：17
• 页码：6139 - 6142
• 全文大小：72K
• 年卷期：v.54,no.17(August 23, 2006)
• ISSN：1520-5118

文摘

A new indirect competitive fluorescence-linked immunosorbent assay (cFLISA) method for thedetection of sulfamethazine (SM₂) in chicken muscle tissue was demonstrated using quantum dots(QDs) as the fluorescence label coupled with secondary antibody. The sensitivity of the cFLISA wascompared with that of the HPLC method. The 50% inhibition value (IC₅₀) and the limit of detection(LOD) of the cFLISA were 7.7 and 1.0 ng/mL, respectively. When SM₂ was spiked at levels of 50,100, and 200 ng/g, recoveries ranged from 80.6% to 117.4%, with a coefficient of variation of 6.9-9.6%. In the incurred sample analysis, the amounts of SM₂ quantified by cFLISA were similar to theresults obtained by the HPLC method. This study shows that cFLISA could be used as a screeningmethod in practice.