Quantitative Bioimaging Analysis of Gonads in olvas-GFP/ST-II YI Medaka (Transgenic Oryzias latipes) Exposed to Ethinylestradiol

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• 作者：Takeshi Hano ; Yuji Oshima ; Masato Kinoshita ; Minoru Tanaka ; Noriko Mishima ; Tetsuji Ohyama ; Takashi Yanagawa ; Yuko Wakamatsu ; Kenjiro Ozato ; Tsuneo Honjo
• 刊名：Environmental Science & Technology
• 出版年：2007
• 出版时间：February 15, 2007
• 年：2007
• 卷：41
• 期：4
• 页码：1473 - 1479
• 全文大小：425K
• 年卷期：v.41,no.4(February 15, 2007)
• ISSN：1520-5851

文摘

This study investigates the adverse and persistent effectsof ethinylestradiol (EE₂) on mature gonads of transgenicolvas-GFP/ST II-YI medaka (Oryzias latipes). The measurementof gonadal size calculating the GFP-fluorescent areawas used as a technique that enabled monitoring gonadsin living specimens by GFP fluorescence. First, maturemedaka were exposed to EE₂ (47.8-522 ng/L) for 4 weeks.The gonads showed a significant reduction of the GFP-fluorescent area and Gonadosomatic Index in males exposedto EE₂ at >216 ng/L and females exposed at 522 ng/L.Histologically, males at all treatments exhibited testis-ovaand additionally, high connective tissue prevalence at216 ng/L. Next, mature male medaka were exposed toEE₂ (43.7-473 ng/L) for 3 weeks and allowed to depurate for6 weeks, to investigate persistent effects of EE₂. Continuousgonad observation showed that GFP began to decline 3weeks after initial exposure to 215 ng/L. After depuration,the gonad's fluorescent areas gradually recovered, withno statistical difference at the end of the depurationperiod; normal spermatogenesis was present in theseindividuals. Alterations in GFP fluorescence clearly indicatethe condition of the gonad in transgenic medaka andthis strain showed a facilitated screening fish model todetect the adverse effects on the gonad by estrogenicchemicals.