The dichloromethane fraction from Mahonia bealei (Fort.) Carr. leaves exerts an anti-inflammatory effect both in vitro and in vivo

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• 作者：Weicheng Hu^a ; ¹ ; Lei Wu^a ; ^b ; ¹ ; Qian Qiang^a ; ¹ ; Lilian Ji^a ; Xinfeng Wang^a ; Haiqing Luo^a ; Haifeng Wu^c ; Yunyao Jiang^d ; Gongcheng Wang^e ; ^{wgc1955@sina.com" class="auth_mail" title="E-mail the corresponding author} ; Ting Shen^a ; ^{shenting1019@163.cm" class="auth_mail" title="E-mail the corresponding author}
• 关键词：AP-1 ; activator protein-1 ; BALF ; bronchoalveolar lavage fluid ; ELISA ; enzyme-linked immunosorbent assay ; IRF-3 ; phosphoinositide-3-kinase-3 ; LPS ; lipopolysaccharide ; MAPKs ; mitogen-activated protein kinases ; MBL-CH ; dichloromethane fraction from Mahonia bealei leaves ; NF-κB ; nuclear factor &ndash ; κB ; NO ; nitric oxide ; PGE2 ; prostaglandin E2 ; PI3K ; phosphoinositide-3-kinase ; PVDF ; polyvinylidene fluoride ; TLR4 ; toll-like receptor 4
• 刊名：Journal of Ethnopharmacology
• 出版年：2016
• 出版时间：21 July 2016
• 年：2016
• 卷：188
• 期：Complete
• 页码：134-143
• 全文大小：1002 K

文摘

Mahonia bealei has a long history of medical use in traditional Chinese medicine for the treatment of inflammatory-associated diseases. Despite numerous phytochemical and pharmacological studies, there is a lack of systematic studies to understand the cellular and molecular mechanisms of the anti-inflammatory activity of this plant.

Aim of study

This study aimed to evaluate the anti-inflammatory activity of the dichloromethane fraction from M. bealei leaves (MBL-CH).

Materials and methods

RAW 264.7 cells were pretreated with different concentrations of MBL-CH for 30 min prior to treatment with 1 μg/ml of lipopolysaccharide (LPS). The nuclear factor κB (NF-κB) pathway and subsequent production of inflammatory mediators, such as nitric oxide (NO), prostaglandin E₂ (PGE₂), and tumour necrosis factor (TNF)-α were investigated. Furthermore, the in vivo mouse model of LPS-induced acute lung injury (ALI) was employed to study the anti-inflammatory effects of MBL-CH.

Results

Pre-treatment with MBL-CH significantly inhibited the LPS-stimulated secretion of NO, PGE₂, and TNF-α into the culture medium, as well as the mRNA levels of inducible nitric oxide synthase (iNOS), cyclooxygenase-2 (COX-2), and TNF-α, which were associated with a reduction in the phosphorylation of IκBα, Akt, and PI3K and inhibition of the transcriptional activity of NF-κB. Furthermore, in vivo experiments revealed that MBL-CH attenuated LPS-stimulated lung inflammation in mice.

Conclusion

Taken together, our findings indicate that MBL-CH attenuates LPS-stimulated inflammatory responses in macrophages by blocking NF-κB activation through interference with activation of the PI3K/Akt pathway, providing scientific evidence that the plant can be employed in traditional remedies.