Quantitative analysis of intracellular nucleoside triphosphates and other polar metabolites using ion pair reversed-phase liquid chromatography coupled with tandem mass spectrometry
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- 作者：Jianmei Wua ; Yingtao Zhanga ; Richard Wieganda ; Jian Wangb ; Gerold Beplera ; Jing Lia ; lijin@karmanos.org" class="auth_mail" title="E-mail the corresponding author
- 关键词：Ion pair chromatography ; Ribonucleoside triphosphate (NTP) ; Deoxyribonucleoside triphosphate (dNTP) ; LC-MS/MS ; Metabolomics
- 刊名：Journal of Chromatography B, Analytical Technologies in the Biomedical and Life Sciences
- 出版年：2015
- 出版时间：1 December 2015
- 年：2015
- 卷：1006
- 期：Complete
- 页码：167-178
- 全文大小：2828 K
文摘
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An ion pair LC-MS/MS method using trimethylamine–hexafluoroisopropanol.
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Validated for the quantitation of intracellular nucleoside triphosphates.
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Broad application potential for profiling of a variety of highly polar metabolites.
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