The hydrocarbon accumulation conditions in the Huanghekou depression are analyzed in this paper, using the latest high-resolution 3D seismic and well logging, combined with the core observation information, from the source rock, reservoirs, reservoir-seal assemblage, traps type and carrier systems.The results show that source rocks are general in the Huanghekou depression, and there develop three sets of hydrocarbon source rocks, and the 3rd Member of the Shahejie Formation and the 3rd Member of the Dongying Formation are the major source rocks. The main reservoir includes Cenozoic clastic reservoir and bedrock reservoir. The main types of source-reservoir-cap rock assemblages include lower-generation middle-accumulation and upper-cap rock, upper-generation lower-accumulation and upper-cap rock, indigenous generation and accumulation.