Based on detailed hydrogeological survey of water supply of Dusitu River Valley in Ordos Basin, aquifer structure and buried depth were investigated. The changes of aquifer and confining bed in horizontal and vertical directions were discussed. The results showed that the main supplies for the three water-rich areas were lateral runoff and atmospheric water infiltration, and the main drainages were phreatic water table evaporation and draining into river. According to the water inflow of single well in different aquifers, tubular well exploitation mode and partially penetrated well structure in middle part suspension were selected, and pattern well spacing was average, and depth of well was 300 m, and the groundwater only in Huanhe Formation of Cretaceous was exploited.