This paper presents a new method for calculating vapour/liquid ratios of fluid inclusions based on high-resolution petrographic microscopy and fluorescent microscopy. The microscope can offer a series of two-dimensional images of single fluid inclusions by regulating its own dial. The volume of inclusion and vapour bubble can then be determined by 1) accumulating the slices which are described as irregular cylinders using equation; and 2) integrating the pseudo-function of area and depth. The vapour/liquid ratio can then be calculated. The new method can provide vapour/liquid ratios of not only fluorescent hydrocarbon-bearing fluid inclusions but also non-fluorescent aqueous fluid inclusions.