摘要
•Scored tens of thousands of SNP markers across guayule, mariola, and wild quinine.•Identified at least two distinct genetic sources of guayule germplasm.•Interspecific Parthenium hybrids had multiple ploidy levels and high aneuploidy.•Discovered cryptic interspecific hybrids within wild and cultivated accessions.•Wild quinine has a ∼1.5-fold larger nuclear genome size than guayule or mariola.