超高效合相色谱-四极杆飞行时间质谱联用技术快速分析广西莪术化学成分
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摘要
采用超临界流体萃取(SFE)技术提取广西莪术根茎,应用超高效合相色谱-四极杆飞行时间质谱联用技术(UPC~2-QTOF-MS)分析其提取物,结合UNIFI软件的内置中药数据库和自建的姜黄属植物化学成分数据库筛查,快速分离鉴定广西莪术SFE提取物中的化学成分。本研究初步鉴定出广西莪术SFE提取物中47种化合物,其中42种是倍半萜类化合物。47种化合物中有8组同分异构体化合物,共37种化合物。UPC~2采用Torus Diol 130?色谱柱分离,流动相A为CO_2,流动相B为甲醇/异丙醇(3:1,V/V)+0.1%甲酸,进行梯度洗脱,在7 min内实现了47种化合物的分离。本方法无需复杂的样品前处理过程,分析速度快、试剂用量少、成本低、环保,是一种全面高效的分析方法,为中药的化学成分分析提供了新方法。
Rapid identification of chemical constituents responsible for defined bioactivities from traditional Chinese medicines(TCMs) is a great challenge. A strategy for rapid identification of the chemical ingredients from the extracts of the rhizoma of Curcuma kwangsiensis with supercritical fluid extraction(SFE) was proposed using ultra-performance convergence chromatography coupled with a quadrupole time-of-flight mass spectrometry(UPC~2-QTOF-MS) combined with UNIFI library. In this study, a total of 47 chemical compounds which were separated in 7 min were tentatively identified, including 42 sesquiterpenes. There were 37 chemical constituents in 8 groups of isomers in the analysis. The strategy which can rapidly, effectively, and comprehensively screen and identify components from TCMs has potential application in TCMs analysis as an alternative method besides UPLC-QTOF-MS and GC-MS.
Rapid identification of chemical constituents responsible for defined bioactivities from traditional Chinese medicines(TCMs) is a great challenge. A strategy for rapid identification of the chemical ingredients from the extracts of the rhizoma of Curcuma kwangsiensis with supercritical fluid extraction(SFE) was proposed using ultra-performance convergence chromatography coupled with a quadrupole time-of-flight mass spectrometry(UPC~2-QTOF-MS) combined with UNIFI library. In this study, a total of 47 chemical compounds which were separated in 7 min were tentatively identified, including 42 sesquiterpenes. There were 37 chemical constituents in 8 groups of isomers in the analysis. The strategy which can rapidly, effectively, and comprehensively screen and identify components from TCMs has potential application in TCMs analysis as an alternative method besides UPLC-QTOF-MS and GC-MS.
引文
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2 Li D X,Schmitz O J.Anal.Bioanal.Chem.,2015,407(1):231-240
3 LIU Xuan,YUE Qing-Xi,GUO De-An.Chin.J.Nat.Med.,2009,7(4):260-269刘璇,岳庆喜,果德安.中国天然药物,2009,7(4):260-269
4 Li F S,Weng J K.Nat.Plants,2017,3:17109-17116
5 GAO Wen,SONG Hui-Peng,YANG Hua,LI Ping.Chinese Journal of Chromatography,2017,35(1):121-128高雯,宋慧鹏,杨华,李萍.色谱,2017,35(1):121-128
6 Zhang Z X,Bo T,Bai Y,Ye M,An R,Cheng F F,Liu H W.Tr AC-Trend.Anal.Chem.,2015,72:169-180
7 SONG Wei-Feng,TAO Ling,ZHONG Ming.Journal of Instrumental Analysis,2012,31(7):810-815宋伟峰,陶玲,钟鸣.分析测试学报,2012,31(7):810-815
8 Sun H,Liu C,Zhang A H,Han Y,Yan G L,Wang P,Wang X J.Anal.Methods,2015,7:279-286
9 Fan YY,Man S L,Li H F,Liu Y X,Liu Z,Gao W Y.J.Pharmaceut.Biomed.,2016,120:364-373
10 Zhuang B,Bi Z M,Wang Z Y,Duan L,Lai C J S,Liu E H.J.Pharmaceut.Biomed.,2018,154:207-215
11 Perrenoud A G G,Veuthey J L,Guillarme D.J.Chromatogr.A,2014,1339:174-184
12 Huang Y,Tang G Y,Zhang T T,Fillet M,Crommen J,Jiang Z J.J.Pharmaceut.Biomed.,2018,147:65-80
13 XU Yong-Wei,SUN Qing-Long,HUANG Jing,TAN Xiao-Jie.Mod.Instrum.,2012,18(5):45-48徐永威,孙庆龙,黄静,谭晓杰.现代仪器,2012,18(5):45-48
14 Gourmel C,Perrenoud A G G,Waller L,Reginato E,Verne J,Dulery B,Veuthey J L,Rudaz S,Schappler J,Guillarme D.J.Chromatogr.A,2013,1282:172-177
15 Gong X,Qi N L,Wang X X,Lin L J,Li J H.Food Chem.,2014,162:172-175
16 Guo W Y,Bian Z Y,Zhang D H,Tang G L,Liu W,Wang J L,Li Z H,Yang F.J.Sep.Sci.,2015,38(5):858-863
17 Jiang H,Yang L,Xing X D,Yan M L,Guo X Y,Yang B Y,Wang Q H,Kuang H X.J.Pharmaceut.Biomed.,2018,153:117-125
18 Zhou Q,Gao B Y,Zhang X,Xu Y W,Shi H M,Yu L L(Lucy).Food Chem.,2014,143:199-204
19 Gao B Y,Luo Y H,Lu W Y,Liu J,Zhang Y Q,Yu L L(Lucy).Food Chem.,2017,218:569-574
20 Tu A Q,Ma Q,Bai H,Du Z X.Food Chem.,2017,221:555-567
21 Chinese Pharmacopoeia Commission.Pharmacopoeia of the People's Republic of China.Beijing:China Medical Science Press,2015:274-275国家药典委员会.中华人民共和国药典.北京:中国医药科技出版社,2015:274-275
22 Li J,Zhao F,Li M Z,Chen L X,Qiu F.J.Nat.Prod.,2010,73(10):1667-1671
23 Phan M G,Tran T T N,Phan T S,Matsunami K,Otsuka H.Phytochem.Lett.,2014,9:137-140
24 Xiang F F,He J W,Liu Z X,Peng Q Z,Wei H.Nat.Prod.Res.,2018,32(22):2670-2675
25 Dong C X,Zhang L J,Xu R,Zhang G,Zhou Y B,Han X Q,Zhang Y,Sun Y X.Int.J.Biol.Macromol.,2015,77:99-104
26 Dong C X,Zhang W S,Sun Q L,Jiang Y J,Fu Z,Zhang Y,Du J,Sun Y X,Tao N,Yao Z.Int.J.Biol.Macromol.,2018,115:1233-1240
27 LIN Guo-Biao,SU Jiang-Yu,YANG Xiu-Fen.Chin.J.Exp.Tradit.Med.Form.,2011,17(16):171-173林国彪,苏姜羽,杨秀芬.中国实验方剂学杂志,2011,17(16):171-173
28 ZENG Jian-Hong,MO Xuan-Yong,DAI Ping,HUANG Feng-Xiang,LIAO Ying,WANG Jian-Hong,CHEN Xu.Chin.J.Exp.Tradit.Med.Form.,2012,18(13):91-94曾建红,莫炫永,戴平,黄凤香,廖迎,王建红,陈旭.中国实验方剂学杂志,2012,18(13):91-94
29 Chen S D,Gao J T,Liu J G,Liu B,Zhao R Z,Lu C J.Fitoterapia,2015,102:67-73
30 Yang F Q,Li S P,Zhao J,Lao S C,Wang Y T.J.Pharmaceut.Biomed.,2007,43(1):73-82
31 Zhou W J,Guo Z M,Yu L,Zhou H,Shen A J,Jin Y,Jin G W,Yan J Y,Yang F,Liu Y M,Wang C R,Feng J T,Liu Y F,Liang X M.Talanta,2018,186:73-79
32 Sun W,Wang S,Zhao WW,Wu C H,Guo S H,Gao H W,Tao H X,Lu J J,Wang Y T,Chen X P.Crit.Rev.Food Sci.Nutr.,2017,57(7):1451-1523
33 Deng L,Shi A M,Liu H Z,Meruva N,Liu L,Hu H,Yang Y,Huang Ch,Li P,Wang Q.J.Mass Spectrom.,2016,51(12):1157-1167
34 CHEN Si-Bao,PENG Yong,CHEN Shi-Lin,XIAO Pei-Gen.Mod.J.Tradit.Chin.Med.Mater.Medica.-World Sci.Technol.,2005,7(6):97-103陈四保,彭勇,陈士林,肖培根.世界科学技术-中医药现代化,2005,7(6):97-103
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36 Xin H X,Da Z S,Cai J F,Ke Y X,Shi H,Fu Q,Jin Y,Liang X M.J.Chromatogr.A,2017,1509:141-146
37 Teubel J,Wust B,Schipke C,Peters O,Parr M K.J.Chromatogr.A,2018,1554:101-116