盐胁迫对东北薄荷种子萌发的影响
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摘要
为了研究4种单盐对东北薄荷Mentha sachalinensis(Briq.)Kudo.种子萌发特性的影响,明确种子萌发期对4种盐胁迫的耐受性。人工模拟不同浓度NaCl、Na_2SO_4、NaHCO_3和Na_2CO_3胁迫条件,采用双层滤纸法培养各胁迫处理种子,测定发芽率、发芽势、发芽指数、活力指数、平均发芽时间等发芽指标,并通过相对发芽率与盐浓度建立的函数方程确定东北薄荷种子萌发期对4种盐胁迫的耐盐适宜浓度、耐盐半致死浓度和耐盐极限浓度。结果表明,随着盐浓度的升高,4种盐胁迫处理下的平均发芽时间逐渐延长,发芽率、发芽指数和活力指数逐渐下降;Na_2CO_3胁迫处理下的发芽势呈逐渐下降趋势,而其他3种盐处理下的发芽势均呈现先升高后下降的趋势。东北薄荷种子萌发期对NaCl、Na_2SO_4、NaHCO_3和Na_2CO_3的耐盐适宜浓度为119.44、64.64、64.32和19.27 mmol·L~(-1),耐盐半致死浓度为153.36、66.70、86.54和22.23 mmol·L~(-1),耐盐极限浓度为191.21、105.33、108.07和25.07mmol·L~(-1);东北薄荷种子对4种盐胁迫的耐受性由大到小依次为NaCl、NaHCO_3、Na_2SO_4、Na_2CO_3。
We investigated the effects of four kinds of salt stress on seed germination of Mentha sachalinensis( Briq.) Kudo.,and confirmed the salt-tolerance of seeds during germination period. The salt stress environment was simulated with different concentrations of NaCl,Na_2SO_4,NaHCO_3 and Na_2CO_3,and seeds were cultured adopting a double-layer filter paper method under four kinds of salt stress. Several indexes of seed germination were measured such as germination rate,germination potential,germination index,vigor index and mean germination time,and through the function equation established by relative germination rate and salt concentrations,the salt-tolerance appropriate content,salt-tolerance half-lethal and salt-tolerance limit concentrations of NaCl,Na_2SO_4,NaHCO_3 and Na_2CO_3 were determined at seed germination period of M. sachalinensis. With the increasing salinity,the mean germination time was gradually extended,and the germination rate,germination index and vigor index were all reduced under four kinds of salt stress. Under Na_2CO_3 stress,the germination potential was decreased with increasing salinity,while the germination potential was increased,and then decreased under NaCl,Na_2SO_4 and NaHCO_3 stress. The appropriate contents of salt-tolerance for NaCl,Na_2SO_4,NaHCO_3 and Na_2CO_3 were 119.44,64.64,64.32 and 19.27 mmol·L~(-1) at seed germination period,the salt-tolerance half-lethal concentrations were 153.36,66.70,86.54 and 22.23 mmol·L~(-1),and the salt-tolerance limit concentrations were 191.21,105.33,108.07 and 25.07 mmol·L~(-1),respectively. The descending tolerance order of M. sachalinensis seeds for four kinds of salt stress was NaCl,NaHCO_3,Na_2SO_4 and Na_2CO_3.
We investigated the effects of four kinds of salt stress on seed germination of Mentha sachalinensis( Briq.) Kudo.,and confirmed the salt-tolerance of seeds during germination period. The salt stress environment was simulated with different concentrations of NaCl,Na_2SO_4,NaHCO_3 and Na_2CO_3,and seeds were cultured adopting a double-layer filter paper method under four kinds of salt stress. Several indexes of seed germination were measured such as germination rate,germination potential,germination index,vigor index and mean germination time,and through the function equation established by relative germination rate and salt concentrations,the salt-tolerance appropriate content,salt-tolerance half-lethal and salt-tolerance limit concentrations of NaCl,Na_2SO_4,NaHCO_3 and Na_2CO_3 were determined at seed germination period of M. sachalinensis. With the increasing salinity,the mean germination time was gradually extended,and the germination rate,germination index and vigor index were all reduced under four kinds of salt stress. Under Na_2CO_3 stress,the germination potential was decreased with increasing salinity,while the germination potential was increased,and then decreased under NaCl,Na_2SO_4 and NaHCO_3 stress. The appropriate contents of salt-tolerance for NaCl,Na_2SO_4,NaHCO_3 and Na_2CO_3 were 119.44,64.64,64.32 and 19.27 mmol·L~(-1) at seed germination period,the salt-tolerance half-lethal concentrations were 153.36,66.70,86.54 and 22.23 mmol·L~(-1),and the salt-tolerance limit concentrations were 191.21,105.33,108.07 and 25.07 mmol·L~(-1),respectively. The descending tolerance order of M. sachalinensis seeds for four kinds of salt stress was NaCl,NaHCO_3,Na_2SO_4 and Na_2CO_3.
引文
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[15]杨春武,李长有,张美丽,等.盐、碱胁迫下小冰麦体内的p H及离子平衡[J].应用生态学报,2008,19(5):1000-1005.
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[17]罗君,彭飞,王涛,等.黑果枸杞(Lycium ruthenicum)种子萌发及幼苗生长对盐胁迫的响应[J].中国沙漠,2017,37(2):261-267.
[18]唐华江,赵丽丽,陈超,等.不同处理方法对2种雀稗属牧草种子萌发的影响[J].种子,2017,36(2):73-77.
[19]曹满航,李进,张婷,等.温度和水分及盐分胁迫对银沙槐种子萌发的影响[J].西北植物学报,2011,31(4):746-753.
[20]张绍山,刘璇,王景富,等.多因素处理对云南重楼及其多芽品系种子萌发的影响[J].中草药,2017,48(10):2111-2115.
[21]姜云天,张丽娜,顾地周,等.盐胁迫对茶花凤仙种子萌发的影响[J].东北林业大学学报,2014,42(3):37-41.
[22]刘玉艳,王辉,于凤鸣,等.盐胁迫对二色补血草种子萌发的影响[J].生态学杂志,2009,28(9):1794-1800.
[23]FANG Y J,LI J,JIANG J J,et al.Physiological and epigenetic analyses of Brassica napus seed germination in response to salt stress[J].Acta Physiologiae Plantarum,2017,39(6):128-139.
[24]徐小玉,张凤银,曾庆微.Na Cl和Na2SO4盐胁迫对波斯菊种子萌发的影响[J].东北林业大学学报,2014,42(4):55-59.
[25]王进,罗光宏,颜霞,等.单盐胁迫对蒙古扁桃种子萌发和幼苗生长的影响[J].中草药,2017,48(12):2509-2515.
[26]王妮妮.混合盐碱胁迫对皂荚种子萌发的影响[J].东北林业大学学报,2017,45(4):14-18,27.
[27]陆玉建,高春明,郑香峰,等.盐胁迫对拟南芥种子萌发的影响[J].湖北农业科学,2012,51(22):5099-5104.
[28]郑安俭,王州飞,张红生.作物种子萌发生理与遗传研究进展[J].江苏农业学报,2017,33(1):218-223.
[29]鱼小军,师尚礼,龙瑞军,等.生态条件对种子萌发影响研究进展[J].草业科学,2006,23(10):44-49.
[30]高暝,李毅,种培芳,等.渗透胁迫下不同地理种源白刺的生理响应[J].草业学报,2011,20(3):99-107.
[31]王志才,牙库甫江·阿西木,王艳,等.水盐胁迫对花花柴种子萌发的影响[J].中国沙漠,2012,32(3):750-755.
[32]祖悦.中性盐和碱性盐胁迫对香豌豆和茶花凤仙的影响[D].牡丹江:牡丹江师范学院,2016.
[33]庄华蓉.单盐和混合盐胁迫对沙冬青种子萌发的影响[J].节水灌溉,2015(2):15-20.
[34]庞克坚,宋占丽,赵晓英.盐胁迫对蓝蓟种子萌发及早期幼苗的影响[J].中国野生植物资源,2012,31(5):37-40.
[2]TALEI D,VALDIANI A,YUSOP M K,et al.Estimation of salt tolerance in Andrographis paniculata accessions using multiple regression model[J].Euphytica,2013,189(1):147-160.
[3]万春阳,王丹,侯俊玲,等.氯化钠胁迫对甘草生长、生理及有效成分含量的影响[J].中国实验方剂学杂志,2011,17(18):118-122.
[4]吉林省中医中药研究所.长白山植物药志[M].长春:吉林人民出版社,1982.
[5]剑桂新,周自新,周荣汉.东北薄荷的化学型[J].植物资源与环境学报,1995,4(4):60-62.
[6]李相来.东北薄荷的种子繁育技术[J].农业与技术,2013,33(12):96.
[7]张侠,宋莉璐,任艳,等.椒样薄荷对Na Cl胁迫的生理响应[J].安徽农业科学,2009,37(13):5967-5969.
[8]LI Z,WANG W W,LI G L,et al.MAPK-mediated regulation of growth and essential oil composition in a salt-tolerant peppermint(Mentha piperita L.)under Na Cl stress[J].Protoplasma,2016,253(6):1541-1556.
[9]SHARIATMADARI Z,RIAHI H,ABDI M,et al.Impact of cyanobacterial extracts on the growth and oil content of the medicinal plant Mentha piperita L.[J].Journal of Applied Phycology,2015,27(6):2279-2287.
[10]OUESLATI S,KARRAY-BOURAOUI N,ATTIA H,et al.Physiological and antioxidant responses of Mentha pulegium(Pennyroyal)to salt stress[J].Acta Physiologiae Plantarum,2010,32(2):289-296.
[11]BHARTI N,BARNAWAL,AWASTHI A,et al.Plant growth promoting rhizobacteria alleviate salinity induced negative effects on growth,oil content and physiological status in Mentha arvensis[J].Acta Physiologiae Plantarum,2014,36(1):45-60.
[12]陈连官.薄荷留兰香品种耐盐特性研究[J].上海农业科技,2006(4):25-26.
[13]HADDADI B S,HASSANPOUR H,NIKNAM V.Effect of salinity and waterlogging on growth,anatomical and antioxidative responses in Mentha aquatica L.[J].Acta Physiologiae Plantarum,2016,38(5):119-129.
[14]姚荣江,杨劲松,刘广明.东北地区盐碱土特征及其农业生物治理[J].土壤,2006,38(3):256-262.
[15]杨春武,李长有,张美丽,等.盐、碱胁迫下小冰麦体内的p H及离子平衡[J].应用生态学报,2008,19(5):1000-1005.
[16]MILLER T.Effects of emergence time on survival and growth in an early old-field plant community[J].Oecologia,1987,72(2):272-278.
[17]罗君,彭飞,王涛,等.黑果枸杞(Lycium ruthenicum)种子萌发及幼苗生长对盐胁迫的响应[J].中国沙漠,2017,37(2):261-267.
[18]唐华江,赵丽丽,陈超,等.不同处理方法对2种雀稗属牧草种子萌发的影响[J].种子,2017,36(2):73-77.
[19]曹满航,李进,张婷,等.温度和水分及盐分胁迫对银沙槐种子萌发的影响[J].西北植物学报,2011,31(4):746-753.
[20]张绍山,刘璇,王景富,等.多因素处理对云南重楼及其多芽品系种子萌发的影响[J].中草药,2017,48(10):2111-2115.
[21]姜云天,张丽娜,顾地周,等.盐胁迫对茶花凤仙种子萌发的影响[J].东北林业大学学报,2014,42(3):37-41.
[22]刘玉艳,王辉,于凤鸣,等.盐胁迫对二色补血草种子萌发的影响[J].生态学杂志,2009,28(9):1794-1800.
[23]FANG Y J,LI J,JIANG J J,et al.Physiological and epigenetic analyses of Brassica napus seed germination in response to salt stress[J].Acta Physiologiae Plantarum,2017,39(6):128-139.
[24]徐小玉,张凤银,曾庆微.Na Cl和Na2SO4盐胁迫对波斯菊种子萌发的影响[J].东北林业大学学报,2014,42(4):55-59.
[25]王进,罗光宏,颜霞,等.单盐胁迫对蒙古扁桃种子萌发和幼苗生长的影响[J].中草药,2017,48(12):2509-2515.
[26]王妮妮.混合盐碱胁迫对皂荚种子萌发的影响[J].东北林业大学学报,2017,45(4):14-18,27.
[27]陆玉建,高春明,郑香峰,等.盐胁迫对拟南芥种子萌发的影响[J].湖北农业科学,2012,51(22):5099-5104.
[28]郑安俭,王州飞,张红生.作物种子萌发生理与遗传研究进展[J].江苏农业学报,2017,33(1):218-223.
[29]鱼小军,师尚礼,龙瑞军,等.生态条件对种子萌发影响研究进展[J].草业科学,2006,23(10):44-49.
[30]高暝,李毅,种培芳,等.渗透胁迫下不同地理种源白刺的生理响应[J].草业学报,2011,20(3):99-107.
[31]王志才,牙库甫江·阿西木,王艳,等.水盐胁迫对花花柴种子萌发的影响[J].中国沙漠,2012,32(3):750-755.
[32]祖悦.中性盐和碱性盐胁迫对香豌豆和茶花凤仙的影响[D].牡丹江:牡丹江师范学院,2016.
[33]庄华蓉.单盐和混合盐胁迫对沙冬青种子萌发的影响[J].节水灌溉,2015(2):15-20.
[34]庞克坚,宋占丽,赵晓英.盐胁迫对蓝蓟种子萌发及早期幼苗的影响[J].中国野生植物资源,2012,31(5):37-40.