阉割对延边黄牛脂肪沉积的影响及相关基因表达研究
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摘要
本试验通过研究阉割对延边黄牛脂肪沉积的影响及相关基因的表达,试图探索肉牛脂肪沉积的影响因素和分子机制。选择年龄、体重相近,相同条件下饲养的延边黄牛公牛和阉牛各15头,育肥至36月龄屠宰,测定脂肪沉积相关性状,并分析ANGPTL4、DGAT2和FABP4基因mRNA的表达变化。结果表明,延边黄牛阉牛肌内脂肪含量显著高于公牛(P<0.05),背膘厚极显著高于公牛(P<0.01),大理石花纹等级显著高于公牛(P<0.05),说明阉割处理后的延边黄牛胴体和肌内脂肪沉积量显著增加。阉牛的ANGPTL4和DGAT2基因的mRNA表达量显著高于公牛,FABP4基因mRNA表达量极显著高于公牛(P<0.01),说明阉割处理的延边黄牛ANGPTL4、DGAT2和FABP4基因的mRNA表达量增加,促进了脂肪沉积。
The effect of castration on fatty deposition and the expression of related genes in Yanbian Yellow Cattle were studied in this experiment, and the influential factor and molecule mechanism of fatty deposits in beef cattle were further explored. 15 Yanbian Yellow Cattles and 15 steers with the similar age and same conditions were slaughtered until fattening to 36 months. Related character of fat deposition were determined, and mRNA expression of the ANGPTL4, DGAT2 and FABP4 were analyzed. The results showed that intramuscular fat content of the steer was significantly higher than the bulls'(P<0.05), backfat thickness was significantly higher than that of the bull(P<0.01), marbling level was significantly higher than the bulls'(P<0.05), which indicated that the carcass and intramuscular fat deposition of Yanbian Yellow Cattle obviously increased after castration. Steer ANGPTL4 and DGAT2 gene mRNA expression quantity were significantly higher than the bulls', steer FABP4 gene mRNA expression quantity was significantly higher than that of the bull(P<0.01), which indicated that the increasing of ANGPTL4, DGAT2 and FABP4 gene expression quantity in Yanbian Yellow Cattle after castration made contributions to fat deposition.
The effect of castration on fatty deposition and the expression of related genes in Yanbian Yellow Cattle were studied in this experiment, and the influential factor and molecule mechanism of fatty deposits in beef cattle were further explored. 15 Yanbian Yellow Cattles and 15 steers with the similar age and same conditions were slaughtered until fattening to 36 months. Related character of fat deposition were determined, and mRNA expression of the ANGPTL4, DGAT2 and FABP4 were analyzed. The results showed that intramuscular fat content of the steer was significantly higher than the bulls'(P<0.05), backfat thickness was significantly higher than that of the bull(P<0.01), marbling level was significantly higher than the bulls'(P<0.05), which indicated that the carcass and intramuscular fat deposition of Yanbian Yellow Cattle obviously increased after castration. Steer ANGPTL4 and DGAT2 gene mRNA expression quantity were significantly higher than the bulls', steer FABP4 gene mRNA expression quantity was significantly higher than that of the bull(P<0.01), which indicated that the increasing of ANGPTL4, DGAT2 and FABP4 gene expression quantity in Yanbian Yellow Cattle after castration made contributions to fat deposition.
引文
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[14]徐畅,宋霁轩,庞喆,等.延边黄牛ANGPTL4基因组织表达规律与肌内脂肪沉积相关性研究[J].中国畜牧杂志,2017,53(10):43-46.
[15]王国富,高树新,张立凡,等.金华猪DGAT1、DGAT2基因发育性表达特点及其与肌内脂肪的关联分析[J].华北农学报,2011,26(5):25-28.
[16]Jeong J,Kwon E G,Im S K,et al.Expression of fat deposition and fat removal genes is associated with intramuscular fat content in longissimus dorsi muscle of Korean cattle steers[J].Journal of Animal Science,2012,90(6):2044-2053.
[17]Cho S,Park T S,Yoon D H,et al.Identification of genetic polymorphisms in FABP3 and FABP4 and putative association with back fat thickness in Korean native cattle.[J].Bmb Reports,2008,41(1):29-34.
[18]Barendse W,Bunch R J,Thomas M B,et al.A splice site single nucleotide polymorphism of the fatty acid binding protein 4,gene appears to be associated with intramuscular fat deposition in longissimus muscle in Australian cattle[J].Animal Genetics,2009,40(5):770-773.
[19]Hoashi S,Hinenoya T,Tanaka A,et al.Association between fatty acid compositions and genotypes of FABP4 and LXR-alpha,in Japanese Black cattle[J].Bmc Genetics,2008,39(1):1-7.
[20]Shin S C,Heo J P,Chung E R.Genetic variants of the FABP4gene are associated with marbling scores and meat quality grades in Hanwoo(Korean cattle)[J].Molecular Biology Reports,2012,39(5):5323-5330.
[2]Nielsena B K,Thamsborgb S M.Welfare,health and product quality in organic beef production:a Danish perspective[J].Livestock Production Science,2005,94(1-2):41-50.
[3]Anderson L,Mcternan P,Barnett A,et al.The effects of androgens and estrogens on preadipocyte proliferation in human adipose tissue:influence of gender and site[J].Journal of Clinical Endocrinology&Metabolism,2001,86(10):5045-5051.
[4]Christoffersen B O,Gade L P,Golozoubova V,et al.Influence of castration-induced testosterone and estradioldeficiency on obesity and glucose metabolism in male G?ttingen minipigs[J].Steroids,2010,75(10):676-684.
[5]蔡兆伟,陈民利,张立凡,等.去势对猪脂肪沉积及其关键基因表达的影响[J].农业生物技术学报,2013,21(10):1203-1209.
[6]Dijk W,Kersten S.Regulation of lipid metabolism by angiopoietin-like proteins[J].Curr Opin Lipidol,2016,27(3):249-256.
[7]Dijk W,Schutte S,Aarts E O,et al.Regulation of angiopoietinlike 4 and lipoprotein lipase in human adipose tissue[J].Journal of Clinical Lipidology,2018,12(3):773-783.
[8]闫雷,林家栋,刘若余,等.DGAT基因在畜禽育种中的应用[J].贵州农业科学,2010,38(1):138-142.
[9]吴光松,燕志宏,林鹏飞,等.RNA干扰DGAT1和DGAT2在猪肝细胞和肌内前体脂肪细胞中表达的研究[J].中国畜牧杂志,2018,54(7):91-96.
[10]许瑞霞,高磊,赵伟利,等.FABP4基因在阿勒泰羊尾脂沉积与代谢模型中的表达变化规律[J].遗传,2015(2):174-182.
[11]王淑辉.利用基因芯片对阉割引起牛生长发育和脂肪沉积变化分子机理的研究[D].北京:中国农业科学院,2008.
[12]Landon M E,Hedrick H B,Thompson G B.Live animal performance and carcass characteristics of beef bullocks and steers[J].Taiwan Journal of Forest Science,2001,16(2):93-102.
[13]马云.牛三个基因的分离、定位及其与牛经济性状的关联研究[D].杨凌:西北农林科技大学,2006.
[14]徐畅,宋霁轩,庞喆,等.延边黄牛ANGPTL4基因组织表达规律与肌内脂肪沉积相关性研究[J].中国畜牧杂志,2017,53(10):43-46.
[15]王国富,高树新,张立凡,等.金华猪DGAT1、DGAT2基因发育性表达特点及其与肌内脂肪的关联分析[J].华北农学报,2011,26(5):25-28.
[16]Jeong J,Kwon E G,Im S K,et al.Expression of fat deposition and fat removal genes is associated with intramuscular fat content in longissimus dorsi muscle of Korean cattle steers[J].Journal of Animal Science,2012,90(6):2044-2053.
[17]Cho S,Park T S,Yoon D H,et al.Identification of genetic polymorphisms in FABP3 and FABP4 and putative association with back fat thickness in Korean native cattle.[J].Bmb Reports,2008,41(1):29-34.
[18]Barendse W,Bunch R J,Thomas M B,et al.A splice site single nucleotide polymorphism of the fatty acid binding protein 4,gene appears to be associated with intramuscular fat deposition in longissimus muscle in Australian cattle[J].Animal Genetics,2009,40(5):770-773.
[19]Hoashi S,Hinenoya T,Tanaka A,et al.Association between fatty acid compositions and genotypes of FABP4 and LXR-alpha,in Japanese Black cattle[J].Bmc Genetics,2008,39(1):1-7.
[20]Shin S C,Heo J P,Chung E R.Genetic variants of the FABP4gene are associated with marbling scores and meat quality grades in Hanwoo(Korean cattle)[J].Molecular Biology Reports,2012,39(5):5323-5330.