微肥配施对瓦布贝母产量和总生物碱含量的影响
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摘要
为探究锌、硼和钼对瓦布贝母产量和总生物碱成分含量的影响及最适需肥量,采用三因素五水平二次正交旋转组合设计进行田间试验,结合室内分析,分别建立瓦布贝母增重率和总生物碱含量与锌(X_1)、硼(X_2)、钼(X_3)施用量的数学函数模型。结果表明,所建立的回归方程均达到显著水平。通过数据模型选优,施用锌肥8.21~11.49 kg·hm~(-2)、硼肥10.86~16.42 kg·hm~(-2)、钼肥0.47~0.66kg·hm~(-2)时,瓦布贝母增重率大于113.75%;施用锌肥7.30~12.40 kg·hm~(-2)、硼肥11.14~16.14kg·hm~(-2)、钼肥0.31~0.51 kg·hm~(-2)时,总生物碱的含量大于0.43%。综上,在瓦布贝母生产中,应根据土壤肥力状况采用适宜的微肥配比。本研究结果为提高瓦布贝母产量和总生物碱含量提供了理论依据。
In order to explore the effect of different ratios of zinc,boron and molybdenum trace element fertilizer on the content of yield and total alkaloid of Fritillaria unibracteata var. wabuensis. a field experiment was carried out by a composite quadratic orthogonal regressive rotation design of three factors and five levels. Combined with lab experimental analysis,we established the mathematical function models of weight gain rate and the total alkaloid content of Fritillaria wabuensis unibracteata var. with the application rate of zinc( X_1),boron( X_2) and molybdenum( X_3),respectively.The results showed that both the established regression equation reached at significant level. According to the mathematical regression model optimization. The weight gain rate was over 113. 75% when the fertilizer application rates were as follows: 8. 21 ~ 11. 49 kg·hm~(-2) zinc fertilizer,10. 86 ~ 16. 42 kg·hm~(-2) boron fertilizer,0. 47 ~ 0. 66 kg·hm~(-2) molybdenum fertilizer. Additionally,the content of the total alkaloid increased to over 0. 43% when the zinc fertilizer boron fertilizer and molybdenum fertilizer were applied 7. 30 ~ 12. 40 kg·hm~(-2),11. 14 ~ 16. 14 kg·hm~(-2) and 0. 31 ~0. 51 kg·hm~(-2),respectively. In conclusion,it should be based on the soil fertility to use the appropriate ratio of trace element fertilizer in the production of fritillaria wabuensis. This study provided a theoretical basis for improving the yield and total alkaloid content of fritillaria wabuensis by using the corresponding fertilization measures in the process of production.
In order to explore the effect of different ratios of zinc,boron and molybdenum trace element fertilizer on the content of yield and total alkaloid of Fritillaria unibracteata var. wabuensis. a field experiment was carried out by a composite quadratic orthogonal regressive rotation design of three factors and five levels. Combined with lab experimental analysis,we established the mathematical function models of weight gain rate and the total alkaloid content of Fritillaria wabuensis unibracteata var. with the application rate of zinc( X_1),boron( X_2) and molybdenum( X_3),respectively.The results showed that both the established regression equation reached at significant level. According to the mathematical regression model optimization. The weight gain rate was over 113. 75% when the fertilizer application rates were as follows: 8. 21 ~ 11. 49 kg·hm~(-2) zinc fertilizer,10. 86 ~ 16. 42 kg·hm~(-2) boron fertilizer,0. 47 ~ 0. 66 kg·hm~(-2) molybdenum fertilizer. Additionally,the content of the total alkaloid increased to over 0. 43% when the zinc fertilizer boron fertilizer and molybdenum fertilizer were applied 7. 30 ~ 12. 40 kg·hm~(-2),11. 14 ~ 16. 14 kg·hm~(-2) and 0. 31 ~0. 51 kg·hm~(-2),respectively. In conclusion,it should be based on the soil fertility to use the appropriate ratio of trace element fertilizer in the production of fritillaria wabuensis. This study provided a theoretical basis for improving the yield and total alkaloid content of fritillaria wabuensis by using the corresponding fertilization measures in the process of production.
引文
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[13]伍燕华.川贝母(Fritillaria cirrhosa)栽培中关键技术的初步研究[D].成都:成都中医药大学,2013
[14]Pan F,Wu W.Simultaneous determination of 10 nucleosides and nucleobases from different cultivation years of Fritillaria unibracteata var.wabuensis by HPLC-DAD[J].Journal of Chinese Pharmaceutical Sciences,2017,26(5):345-365
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[16]Wu X,Chan S W,Ma J,Ping L,Shaw P C,Ge L.Investigation of association of chemical profiles with the tracheobronchial relaxant activity of Chinese medicinal herb Beimu derived from various Fritillaria species[J].Journal of Ethnopharmacology,2017,210:39-46
[17]颜晓燕,童志远,晏子俊,罗燕秋,唐丽,吴珊珊,杨军,彭成.暗紫贝母、栽培瓦布贝母及浙贝母药效学比较[J].中国实验方剂学杂志,2012,18(10):244-248
[18]Wang D,Chen X,Atanasov A G,Xiao Y,Shu W.Plant resource availability of medicinal fritillaria species in traditional producing regions in Qinghai-Tibet Plateau[J].Frontiers in Pharmacology,2017,8:1-10
[19]苏鹏,胡莉,董品利.八个川贝母品种的分类鉴定[J].西南农业学报,2014,27(6):2559-2563
[20]杨晓云,陈良.叶片吸肥特性及叶面肥施用技术[J].吉林农业,2011(8):92
[21]张耀鸿.微量元素肥料的功能以及施用方法[J].安徽农学通报,2007,13(19):120-121
[22]蒋向辉,佘朝文,苑静.微量元素对金银花绿原酸合成关键酶基因Lj HCT和Lj C3H1表达的影响研究[J].植物科学学报,2017,35(2):642-649
[23]窦明明,石峰,马留辉,冯宇,张亚琴,李雨杺,陈兴福.微肥配施对泽泻产量的影响[J].中药材,2017,40(1):7-11
[24]张勇强,宋航,薛志伟,牛平平,介领军,李潮海.施用锌肥和硼肥对玉米穗粒性状和品质的影响[J].核农学报,2017,31(2):371-378
[25]张元珍.供锌水平对苹果树叶片光合特性、碳水化合物及其代谢酶活性的影响[D].泰安:山东农业大学,2011
[26]杨系玲,杨克军,李佐同,王玉凤,张翼飞,刘慧迪,唐春双,马振川,张文超,陈天宇.锌对不同基因型玉米幼苗光合特性及锌积累的影响[J].核农学报,2016,30(3):571-579
[27]武松伟,胡承孝,谭启玲,孙学成,赵小虎,庄光泉,袁迎春.施钼对冬小麦钼高、低效品种氮代谢的影响[J].华中农业大学学报,2015,34(5):52-57
[28]张翔,毛家伟,司贤宗,李国平.不同种类有机肥与钼肥配施对连作花生生长发育及产量、品质的影响[J].中国油料作物学报,2014,36(4):489-493
[2]张培培,周勤梅,秦波,姚志昂,彭城,郭力.川贝母栽培品瓦布贝母与松贝母的总生物碱对比研究[J].成都中医药大学学报,2011,34(1):70-72
[3]王娟娟,曹博,白成科,张琳琳,车乐.基于Maxent和Arc GIS预测川贝母潜在分布及适宜性评价[J].植物研究,2014,34(5):642-649
[4]刘震东,王曙,陈心启.关于瓦布贝母的分类等级研究[J].植物分类与资源学报,2009,31(2):145,182
[5]刘辉,陈士林,姚辉,李西文,张艺.川贝母的资源学研究进展[J].中国中药杂志,2008,33(14):1645-1648
[6]张大永,王曙,李庆,刘震东,王聪.栽培川贝母采收年限的研究[J].华西药学杂志,2010,25(6):725-726
[7]毛艳苹,赵高琼,苏玉萍,庞亚,童婷婷,邓赟.川产贝母新资源瓦布贝母研究进展[J].中药与临床,2014,5(3):53-55
[8]林丽君,钟燕珠,雷旭,区炳雄,林华.HPLC-ELSD法测定西藏林芝地区野生瓦布贝母中3种生物碱的含量[J].广东药学院学报,2016,32(4):458-461
[9]潘峰,侯凯,董品利,陈郡雯,胡博,吴卫.HPLC-ELSD法同时检测瓦布贝母三种生物碱的含量[J].核农学报,2014,28(10):1854-1859
[10]郑军.人工栽培濒危药用植物川贝母鳞茎质量研究[D].雅安:四川农业大学,2009
[11]马靖.栽培川贝母品质调控技术的初步研究[D].成都:成都中医药大学,2015
[12]孟杰,陈兴福,杨文钰,李志飞,张钰,宋九华,杨兴旺.锌硼钼配施对北柴胡产量及柴胡皂苷a,d含量的影响[J].中国中药杂志,2014,39(22):4297-4303
[13]伍燕华.川贝母(Fritillaria cirrhosa)栽培中关键技术的初步研究[D].成都:成都中医药大学,2013
[14]Pan F,Wu W.Simultaneous determination of 10 nucleosides and nucleobases from different cultivation years of Fritillaria unibracteata var.wabuensis by HPLC-DAD[J].Journal of Chinese Pharmaceutical Sciences,2017,26(5):345-365
[15]赵高琼,任波,张乐乐,向晖,杨江,董小萍.瓦布贝母茎叶和鳞茎中生物碱的对比[J].中成药,2013,35(8):1729-1732
[16]Wu X,Chan S W,Ma J,Ping L,Shaw P C,Ge L.Investigation of association of chemical profiles with the tracheobronchial relaxant activity of Chinese medicinal herb Beimu derived from various Fritillaria species[J].Journal of Ethnopharmacology,2017,210:39-46
[17]颜晓燕,童志远,晏子俊,罗燕秋,唐丽,吴珊珊,杨军,彭成.暗紫贝母、栽培瓦布贝母及浙贝母药效学比较[J].中国实验方剂学杂志,2012,18(10):244-248
[18]Wang D,Chen X,Atanasov A G,Xiao Y,Shu W.Plant resource availability of medicinal fritillaria species in traditional producing regions in Qinghai-Tibet Plateau[J].Frontiers in Pharmacology,2017,8:1-10
[19]苏鹏,胡莉,董品利.八个川贝母品种的分类鉴定[J].西南农业学报,2014,27(6):2559-2563
[20]杨晓云,陈良.叶片吸肥特性及叶面肥施用技术[J].吉林农业,2011(8):92
[21]张耀鸿.微量元素肥料的功能以及施用方法[J].安徽农学通报,2007,13(19):120-121
[22]蒋向辉,佘朝文,苑静.微量元素对金银花绿原酸合成关键酶基因Lj HCT和Lj C3H1表达的影响研究[J].植物科学学报,2017,35(2):642-649
[23]窦明明,石峰,马留辉,冯宇,张亚琴,李雨杺,陈兴福.微肥配施对泽泻产量的影响[J].中药材,2017,40(1):7-11
[24]张勇强,宋航,薛志伟,牛平平,介领军,李潮海.施用锌肥和硼肥对玉米穗粒性状和品质的影响[J].核农学报,2017,31(2):371-378
[25]张元珍.供锌水平对苹果树叶片光合特性、碳水化合物及其代谢酶活性的影响[D].泰安:山东农业大学,2011
[26]杨系玲,杨克军,李佐同,王玉凤,张翼飞,刘慧迪,唐春双,马振川,张文超,陈天宇.锌对不同基因型玉米幼苗光合特性及锌积累的影响[J].核农学报,2016,30(3):571-579
[27]武松伟,胡承孝,谭启玲,孙学成,赵小虎,庄光泉,袁迎春.施钼对冬小麦钼高、低效品种氮代谢的影响[J].华中农业大学学报,2015,34(5):52-57
[28]张翔,毛家伟,司贤宗,李国平.不同种类有机肥与钼肥配施对连作花生生长发育及产量、品质的影响[J].中国油料作物学报,2014,36(4):489-493