基于水稻染色体片段代换系的苗期耐低氮QTL分析
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摘要
【目的】通过定位与水稻Oryza sativa耐低氮相关的数量性状位点(QTL),为今后相关基因的精细定位、克隆以及功能研究奠定基础,也为耐低氮水稻品种的培育提供理论参考。【方法】以Koshihikari (受体)和Nona Bokra(供体)为亲本构建的全基因组单片段代换系作为研究材料,在水稻苗期进行低氮胁迫处理,对水稻株高、根长、根鲜质量、根干质量、茎叶鲜质量、茎叶干质量、总鲜质量、总干质量共8个表型进行相对损失比分析和QTL定位。【结果】定位到2个与水稻低氮胁迫耐受相关的位点,分别是qRL1-1和qRFW2-1,这2个QTL位点分别在低氮胁迫下控制水稻根长和根鲜质量。其中,qRL1-1定位于1号染色体M1-29标记附近,LOD值为2.89,可解释的表型变异为11.23%;qRFW2-1定位于2号染色体M2-225标记附近,LOD值为2.53,可解释的表型变异为9.90%。其他6个表型未检测到相关位点。【结论】初步定位了与低氮胁迫下控制水稻根长、根鲜质量相关基因,为进一步的基因精细定位奠定基础。
【Objective】 To map the quantitative trait loci(QTL) associated with low nitrogen tolerance in rice(Oryza sativa), provide a basis for future fine mapping, cloning and functional characterization of related genes and offer theoretical references for breeding of low nitrogen tolerant rice. 【Method】 A set of chromosomal segment substitution lines which was constructed by crossing Koshihikari(recurrent parent) and Nona Bokra(donor parent) were used as test materials. Low nitrogen stress treatment was applied at rice seedling stage. The relative loss ratios of eight phenotypes including rice plant height, root length, root fresh weight, root dry weight, shoot fresh weight, shoot dry weight, total fresh weight and total dry weight were analyzed, and QTL mapping was performed. 【Result】 Two loci related to low nitrogen tolerance of rice were successfully mapped. These two QTLs were qRL1-1 and qRFW2-1 which were related to root length and root fresh weight respectively under low nitrogen stress. qRL1-1 was closed to the M1-29 marker on chromosome 1 with a LOD score of 2.89 and explained about 11.23% of total phenotypic variance. qRFW2-1 was closed to the M2-225 marker on chromosome 2 with a LOD score of 2.53 and explained about 9.90% of total phenotypic variance. No loci was found for other six phenotypic indexes. 【Conclusion】 QTLs related to root length and root fresh weight under low nitrogen stress are mapped, which lays the foundation for further genetic fine mapping and cloning of the underlying genes.
【Objective】 To map the quantitative trait loci(QTL) associated with low nitrogen tolerance in rice(Oryza sativa), provide a basis for future fine mapping, cloning and functional characterization of related genes and offer theoretical references for breeding of low nitrogen tolerant rice. 【Method】 A set of chromosomal segment substitution lines which was constructed by crossing Koshihikari(recurrent parent) and Nona Bokra(donor parent) were used as test materials. Low nitrogen stress treatment was applied at rice seedling stage. The relative loss ratios of eight phenotypes including rice plant height, root length, root fresh weight, root dry weight, shoot fresh weight, shoot dry weight, total fresh weight and total dry weight were analyzed, and QTL mapping was performed. 【Result】 Two loci related to low nitrogen tolerance of rice were successfully mapped. These two QTLs were qRL1-1 and qRFW2-1 which were related to root length and root fresh weight respectively under low nitrogen stress. qRL1-1 was closed to the M1-29 marker on chromosome 1 with a LOD score of 2.89 and explained about 11.23% of total phenotypic variance. qRFW2-1 was closed to the M2-225 marker on chromosome 2 with a LOD score of 2.53 and explained about 9.90% of total phenotypic variance. No loci was found for other six phenotypic indexes. 【Conclusion】 QTLs related to root length and root fresh weight under low nitrogen stress are mapped, which lays the foundation for further genetic fine mapping and cloning of the underlying genes.
引文
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[13]王玉雯,郭九信,孔亚丽,等.氮肥优化管理协同实现水稻高产和氮肥高效[J].植物营养与肥料学报,2016,22(5):1157-1166.
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[15]阮成江,何祯祥,钦佩.我国农作物QTL定位研究的现状和进展[J].植物学通报,2003(1):10-22.
[16]吕海霞,周广生,丁泽红,等.水稻染色体片段代换系对氮反应的QTL分析[J].分子植物育种,2010,8(6):1074-1081.
[17]GEIOFF G C.Intact-plant screening for tolerance of nutrient deficiency stress[J].Plant Soil,1987,99(1):3-16.
[18]梁永书,周军杰,南文斌,等.水稻根系研究进展[J].植物学报,2016,51(1):98-106.
[19]LIU K,HE A,YE C,et al.Root morphological traits and spatial distribution under different nitrogen treatments and their relationship with grain yield in super hybrid rice[J].Sci Rep,2018,8(1):131-139.
[20]赵春芳,赵凌,张亚东,等.水稻苗期耐低氮相关性状的QTL定位[J].华北农学报,2015,30(6):1-7.
[21]FENG Y,CAO L Y,WU W M,et al.Mapping QTLs for nitrogen-deficiency tolerance at seedling stage in rice(Oryza sativa L.)[J].Plant Breeding,2010,129(6):652-656.
[22]CHO Y I,JIANG W,CHIN J H,et al.Identification of QTLs associated with physiological nitrogen use efficiency in rice[J].Mol Cells,2007,23(1):72-79.
[2]顾骏飞,周振翔,李志康,等.水稻低叶绿素含量突变对光合作用及产量的影响[J].作物学报,2016,42(4):551-560.
[3]BACON P E.Nitrogen fertilization in the environment[J].Biol Rev,1995,32(3):296-348.
[4]邓若磊.水稻铵转运蛋白基因OsAMT1.4和OsAMT5的克隆、表达和遗传转化[D].保定:河北农业大学,2007.
[5]SUN H,QIAN Q,WU K,et al.Heterotrimeric G proteins regulate nitrogen-use efficiency in rice[J].Nat Genet,2014,46(6):652-657.
[6]YANAGISAWA S,AKIYAMA A,KISAKA H,et al.Metabolic engineering with Dof1 transcription factor in plants:Improved nitrogen assimilation and growth under low-nitrogen conditions[J].P Natl Acad Sci USA,2004,101(20):7833-7838.
[7]SHAN Y H,WANG Y L,PAN X B.Mapping of QTLs for nitrogen use efficiency and related traits in rice(Oryza sativa L.)[J].Agr Sci China,2005,4(10):721-727.
[8]王彦荣,代贵金,大杉立,等.水稻苗期氮素吸收及其相关性状的QTL分析[J].中国水稻科学,2010,24(5):463-468.
[9]方萍,陶勤南,吴平.水稻吸氮能力与氮素利用率的QTLs及其基因效应分析[J].植物营养与肥料学报,2001(2):159-165.
[10]YOSHIDA S,FORNO D A,COCK J H,et al.Laboratory manual for physiological studies of rice[M].2nd ed.Los Banos:IRRI,1972:57-63.
[11]WANG J K,WAN X Y,CROSSA J,et al.QTL mapping of grain length in rice(Oryza sativa L.)using chromosome segment substitution lines[J].Genet Res,2006,88(2):93-104.
[12]MCCOUCH S R,CHO Y G,YANO M,et al.Report on QTL nomenclature[J].Rice Genet Newsl,1997,14:11-13.
[13]王玉雯,郭九信,孔亚丽,等.氮肥优化管理协同实现水稻高产和氮肥高效[J].植物营养与肥料学报,2016,22(5):1157-1166.
[14]CHOUDHURY A,KENNEDY I R.Nitrogen fertilizer losses from rice soils and control of environmental pollution problems[J].Commun Soil Sci Plant,2005,36(11/12):1625-1639.
[15]阮成江,何祯祥,钦佩.我国农作物QTL定位研究的现状和进展[J].植物学通报,2003(1):10-22.
[16]吕海霞,周广生,丁泽红,等.水稻染色体片段代换系对氮反应的QTL分析[J].分子植物育种,2010,8(6):1074-1081.
[17]GEIOFF G C.Intact-plant screening for tolerance of nutrient deficiency stress[J].Plant Soil,1987,99(1):3-16.
[18]梁永书,周军杰,南文斌,等.水稻根系研究进展[J].植物学报,2016,51(1):98-106.
[19]LIU K,HE A,YE C,et al.Root morphological traits and spatial distribution under different nitrogen treatments and their relationship with grain yield in super hybrid rice[J].Sci Rep,2018,8(1):131-139.
[20]赵春芳,赵凌,张亚东,等.水稻苗期耐低氮相关性状的QTL定位[J].华北农学报,2015,30(6):1-7.
[21]FENG Y,CAO L Y,WU W M,et al.Mapping QTLs for nitrogen-deficiency tolerance at seedling stage in rice(Oryza sativa L.)[J].Plant Breeding,2010,129(6):652-656.
[22]CHO Y I,JIANG W,CHIN J H,et al.Identification of QTLs associated with physiological nitrogen use efficiency in rice[J].Mol Cells,2007,23(1):72-79.