雌激素受体-β基因多态性对广西壮族绝经后妇女血清护骨素的影响
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摘要
目的了解广西壮族绝经后妇女雌激素受体-β基因多态性对其血清护骨素及骨密度(BMD)的影响。方法对667例广西壮族绝经妇女采用定量超声骨质密度仪检测其右跟骨BMD,早晨空腹采肘静脉血5 ml,PCR-RFLP检测雌激素受体-β基因多态性,用酶联免疫吸附试验测定各基因型对应的血清护骨素。结果而60岁后绝经晚期雌激素受体-βRsa I酶切的r等位基因与同年龄组其他4个基因组比较,护骨素和BMD明显降低(P<0. 05); 65岁后绝经晚期雌激素受体-βAlu I酶切的aa等位基因与同年龄组其他4个基因组比较,护骨素和BMD明显提高(P<0. 05)。结论绝经后骨质疏松防治方面,应该根据雌激素受体不同基因型,采取个体化防治,提高防治效果。
Objective To study the effect of estrogen receptor-β gene polymorphism on serum osteoprotective in postmenopa-usal women of Guangxi Zhuang nationality. Methods The quantitative ultrasonic bone density instrument was used to detect the broad-band ultrasonic attenuation value of right heel bone of 667 postmenopausal women of Guangxi Zhuang nationality. The estrogen receptor-β gene polymorphism was tested by PCR-RFLP,the serum osteoprotective level was tested by ELISA. Results At the early postm-enopausal period,the serum osteoprotective levels and bone mineral density( BMD) in all kinds of estrogen receptor-β gene polymor-phism groups were decreased. However,After 60 years of age,the osteoprotegerin level and BMD in r allelic genomes of estrogen re-ceptor-β Rsa I group were significantly decreased compared with those of the other 4 genomes of the same age group. After 65 years ofage,the osteoprotegerin level and BMD in aa allelic genomes of estrogen receptor-β Rsa I group were significantly increased comparedwith those of the other 4 genomes of the same age group.Conclusions Patients carrying estrogen receptor-β Rsa I restriction gene Pallele have lower osteoprotegerin and bone mineral density,and are more prone to postmenopausal osteoporosis.
Objective To study the effect of estrogen receptor-β gene polymorphism on serum osteoprotective in postmenopa-usal women of Guangxi Zhuang nationality. Methods The quantitative ultrasonic bone density instrument was used to detect the broad-band ultrasonic attenuation value of right heel bone of 667 postmenopausal women of Guangxi Zhuang nationality. The estrogen receptor-β gene polymorphism was tested by PCR-RFLP,the serum osteoprotective level was tested by ELISA. Results At the early postm-enopausal period,the serum osteoprotective levels and bone mineral density( BMD) in all kinds of estrogen receptor-β gene polymor-phism groups were decreased. However,After 60 years of age,the osteoprotegerin level and BMD in r allelic genomes of estrogen re-ceptor-β Rsa I group were significantly decreased compared with those of the other 4 genomes of the same age group. After 65 years ofage,the osteoprotegerin level and BMD in aa allelic genomes of estrogen receptor-β Rsa I group were significantly increased comparedwith those of the other 4 genomes of the same age group.Conclusions Patients carrying estrogen receptor-β Rsa I restriction gene Pallele have lower osteoprotegerin and bone mineral density,and are more prone to postmenopausal osteoporosis.
引文
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2 Luo Z,Liu M,Sun L,et al.Icariin recovers the osteogenic differentiation and bone formation of bone marrow stromal cells from a rat model of estrogen deficiency-induced osteoporosis[J].Mol Med Rep,2015;12(1):382-8.
3 Aydin H,Deyneli O,Yavuz D,et al.Effect of oxidative stress on aorta and tibia osteoprotegerin gene expression in ovariectomized rats[J].Minerva Endocrinol,2011;36(2):107-15.
4 Shoukry A,Shalaby SM,Etewa RL,et al.Association of estrogen receptorβand estrogen-related receptorαgene polymorphisms with bone mineral density in postmenopausal women[J].Mol Cell Biochem,2015;45(2):23-31.
5 Deng W,Han JC,Chen L,et al.Estrogen receptor alpha gene PvuⅡpolymorphism and risk of fracture in postmenopausal women:a metaanalysis[J].Genet Mol Res,2015;14(1):1293-300.
6 Obemayer-Pietsch B,Chararas C,Kotschan S,et al.Genetic background of osteoporosis[J].Acta Med Austriaca,2000;27(1):18-22.
7 Schuit SC,de Jong FH,Stolk L,et al.Estrogen receptor alpha gene polymorphisms are associated with estradiol levels in postmenopausal women[J].Eur J Endocrinol,2005;153(2):327-34.
8 Tural S,Kara N,Alayli G,et al.Association between osteoporosis and polymorphisms of the bone Gla protein,estrogen receptor 1,collagen
1-A1 and calcitonin receptor genes in Turkish postmenopausal women[J].Gene,2013;515(1):167-72.
9李海,陈建海,王金花,等.山茶籽联合雌二醇对去卵巢大鼠骨组织的影响[J].解剖学杂志,2012;35(3):286-8,318.
10 Lin PI,Tai YT,Chan WP,et al.Estrogen/ERαsignaling axis participates in osteoblast maturation via upregulating chromosomal and mitochondrial complex gene expressions[J].Oncotarget,2017;9(1):1169-86.
11 Luo D,Liu Y,Zhou Y,et al.Association between dietary phytoestrogen intake and bone mineral density varied with estrogen receptor alpha gene polymorphisms in southern Chinese postmenopausal women[J].Food Funct,2015;6(6):1977-83.
12 Chen X,Zhi X,Pan P,et al.Matrine prevents bone loss in ovariectomized mice by inhibiting RANKL-induced osteoclastogenesis[J].FASEB J,2017;31(11):4855-65.