基于功能性消化不良大鼠胃肠动力变化研究脏腑图点穴法“首开中焦”的顺序效应
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摘要
目的观察功能性消化不良大鼠胃肠动力的变化,研究不同点穴顺序对大鼠胃肠动力调节作用的差异,探讨脏腑图点穴法"首开中焦"的顺序效应。方法 60只SD大鼠随机分为空白组、模型组、上中焦组、中上焦组、中下焦组,每组12只。除空白组外,其余各组用轻度夹尾刺激+间断饮食法持续造模21天,造模成功后给予相应干预措施,持续14天。观察各组大鼠的行为学、胃电图、胃内残留率和小肠推进率,并检测大鼠胃窦组织中c-kit的表达情况。结果模型组的胃电振幅低于空白组(P<0.05);上中焦组、中下焦组的胃电振幅高于模型组(P<0.05);模型组的胃内残留率高于空白组(P<0.05),小肠推进率低于空白组(P<0.05);中下焦组的胃内残留率低于模型组(P<0.05),小肠推进率高于模型组(P<0.05);手法干预各组比较,中下焦组的小肠推进率高于上中焦组和中上焦组(P<0.05)。模型组大鼠胃窦组织中c-kit的平均光密度值(OD值)低于空白组(P<0.01),c-kit阳性ICC减少、着色变浅;中下焦组的OD值高于模型组(P<0.05),c-kit阳性ICC增多、着色变深。结论脏腑图点穴法能有效调节功能性消化不良大鼠的胃肠动力障碍,增加c-kit表达;以"首开中焦"的中下焦组作用最优。
Objective To observe the changes of gastrointestinal motility in rats with functional dyspepsia(FD), investigate the difference in the effect of regulating gastrointestinal motility following different acupoint pressure sequences and explore the sequence effect of "first opening Middle Jiao" in zang-fu viscera acupoint pressure therapy. Methods 60 SD rats were randomly assigned into the blank group, the model group, the Upper-Middle Jiao group, the Middle-Upper Jiao group and the Middle-Lower Jiao group, 12 rats in each group. Except the blank group, rats in the other groups were treated with mild tail-clamping stimulation plus intermittent diet for 21 days for modeling. Each group was given corresponding intervention for 14 consecutive days after successful modeling. The behavior, electrogastrogram, intragastric residual rate and small intestinal propulsion rate of rats were observed and the expression of c-kit was detected in the gastric antrum tissue in each group. Results The gastric electrical amplitude was lower in the model group than in the blank group(P<0.05); it was higher in the Upper-Middle Jiao and Middle-Lower Jiao groups than that in the model group(P<0.05); the gastric residual rate was higher and the small intestinal propulsion rate was lower in the model group than in the blank group(P<0.05); the gastric residual rate was lower and the small intestinal propulsion rate was higher in the Middle-Lower Jiao group than in the model group(P<0.05); and in the manipulation intervention groups, the small intestinal propulsion rate was higher in the Middle-Lower Jiao group than in the Upper-Middle Jiao and Middle-Upper Jiao groups(P<0.05). The mean optical density(OD) value of c-kit in the gastric antrum tissue was lower in the model group than in the blank group(P<0.01), with decrease in c-kit positive interstitial cells of Cajal(ICCs) and a lighter color. The mean OD value was higher in the Middle-Lower Jiao group than in the model group(P<0.05), with increase in c-kit positive ICCs and a darker color. Conclusion Zang-fu viscera acupoint pressure therapy can effectively treat gastrointestinal motility disorder in FD rats, upregulating the expresion of c-kit and "first opening Middle Jiao"in the Middle-Lower Jiao group shows the best effect.
Objective To observe the changes of gastrointestinal motility in rats with functional dyspepsia(FD), investigate the difference in the effect of regulating gastrointestinal motility following different acupoint pressure sequences and explore the sequence effect of "first opening Middle Jiao" in zang-fu viscera acupoint pressure therapy. Methods 60 SD rats were randomly assigned into the blank group, the model group, the Upper-Middle Jiao group, the Middle-Upper Jiao group and the Middle-Lower Jiao group, 12 rats in each group. Except the blank group, rats in the other groups were treated with mild tail-clamping stimulation plus intermittent diet for 21 days for modeling. Each group was given corresponding intervention for 14 consecutive days after successful modeling. The behavior, electrogastrogram, intragastric residual rate and small intestinal propulsion rate of rats were observed and the expression of c-kit was detected in the gastric antrum tissue in each group. Results The gastric electrical amplitude was lower in the model group than in the blank group(P<0.05); it was higher in the Upper-Middle Jiao and Middle-Lower Jiao groups than that in the model group(P<0.05); the gastric residual rate was higher and the small intestinal propulsion rate was lower in the model group than in the blank group(P<0.05); the gastric residual rate was lower and the small intestinal propulsion rate was higher in the Middle-Lower Jiao group than in the model group(P<0.05); and in the manipulation intervention groups, the small intestinal propulsion rate was higher in the Middle-Lower Jiao group than in the Upper-Middle Jiao and Middle-Upper Jiao groups(P<0.05). The mean optical density(OD) value of c-kit in the gastric antrum tissue was lower in the model group than in the blank group(P<0.01), with decrease in c-kit positive interstitial cells of Cajal(ICCs) and a lighter color. The mean OD value was higher in the Middle-Lower Jiao group than in the model group(P<0.05), with increase in c-kit positive ICCs and a darker color. Conclusion Zang-fu viscera acupoint pressure therapy can effectively treat gastrointestinal motility disorder in FD rats, upregulating the expresion of c-kit and "first opening Middle Jiao"in the Middle-Lower Jiao group shows the best effect.
引文
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[2]曹江鸿,戴晓矞.醒脑开窍针法调神穴针刺顺序的探讨[J].中华针灸电子杂志,2016,5(1):5-8.
[3]蔡英姿,王爱玲.痛证的治疗与针刺顺序[J].特色疗法中国民间疗法,2011,19(8):15.
[4]王煜姣,凌江红,张钰琴,等.复合病因造模法制备功能性消化不良大鼠模型[J].世界华人消化杂志,2014,22(2):210-214.
[5]于文靖,陈苏宁,李慎贤,等.胃痛消痞方对功能性消化不良大鼠血清及胃肠组织中CCK、VIP的影响[J].实用药物与临床,2012,15(8):461-463.
[6]刘淑刚,贾楠,安坤杰.舒肝解郁法治疗术后胃瘫综合征的临床观察[J].中国中医基础医学杂志,2015,21(7):899-900.
[7]裴建卫.调气通腑推拿法治疗便秘型肠易激综合征的临床研究[D].济南:山东中医药大学,2012.
[8]中华医学会消化病学分会胃肠动力学组.中国消化不良的诊治指南(2007)[J].胃肠病学,2008,13(2):114-117.
[9]曾丽君.基于胃Cajal间质细胞自噬探讨柴胡疏肝散促进功能性消化不良大鼠胃动力的作用机制[D].南宁:广西医科大学,2017.
[10]CHEN J D,LIN Z,MCCCALLUM R W.Noninvasive feature-based detection of delayed gastric emptying in humans using neural networks[J].IEEE Trans Biomed Eng,2000,47(3):409-412.
[11]LAMMERS W J,STEPHEN B.Origin and propagation of individual slow waves along the intact feline small intestine[J].Exp Physiol,2008,93(3):334-346.
[12]朱飞叶,谢冠群,徐珊.芍药甘草汤对慢传输型便秘大鼠ICC及肠神经递质的影响[J].中华中医药杂志,2016,31(1):248-251.
[13]VOIOSU T A,GIURCAN R,VOIOSU A M,et al.Functional dyspepsia today[J].Maedica(Buchar),2013,8(8):68-74.
[14]邓晶晶,袁青,汪满霞,等.Cajal间质细胞在胃肠功能紊乱中的研究进展[J].中国针灸,2015,35(10):1033-1038.
[15]谢丽微,夏丽娜,赵志光.C-kit、SCF、Cx43在先天性巨结肠中的表达及作用[J].温州医学院学报,2013,43(5):319-322.
[16]肖星蕾,付亚兰,肖姣,等.脏腑图点穴法对胃肠动力障碍大鼠Cajal间质细胞及抑制性神经递质的影响[J].环球中医药,2018,11(5):661-665.