锁掷酵母粉对糖尿病小鼠肝、肾的保护作用
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摘要
该实验研究了锁掷酵母粉对Ⅱ型糖尿病小鼠肝、肾损伤的影响,并探讨其可能的作用机制。C57BL/6小鼠高脂饲料喂养4周后,连续3 d腹腔注射链脲佐菌素(STZ)100 mg/kg,造成Ⅱ型糖尿病模型,设空白组、模型组和酵母粉组(n=8)。连续灌胃8周后,测定小鼠体质量、饮水量、进食量和空腹血糖(FBG);血清、肝脏、肾脏的超氧化物歧化酶(SOD)活性、丙二醛(MDA)含量;苏木精-伊红(HE)染色观察肝、肾组织病理变化。结果表明,与模型组比较,酵母粉组均可明显抑制血糖值升高;血清、肝、肾中MDA含量明显降低(P<0.05);SOD活性明显升高(P<0.05);HE染色显示酵母粉组肝细胞排列整齐,脂肪变性减弱,淋巴细胞减少;肾小球肥大减弱,系膜区减小。表明酵母粉可提高肝脏、肾脏抗氧化能力,减轻STZ及高血糖引起的肝、肾损害。
The effects of Sporidiobolus pararoseus powder on liver and kidney injury of type 2 diabetic mice were studied, and the possible mechanism was explored. After C57 BL/6 mice were fed with high fat diet for 4 weeks, and injected intraperitoneally with streptozotocin(STZ) 100 mg/kg for 3 d,the model of type Ⅱ diabetes was established. The mice were divided into blank group, model group and yeast powder group(n=8). After continuous gastric perfusion for 8 weeks, the body weight, water intake, food intake, fasting blood glucose(FBG), superoxide dismutase(SOD) activity and malondialdehyde(MDA) content in serum, liver and kidney of mice were determined, and the pathological changes of liver and kidney were observed by hemotoxylin and eosin(HE) staining. The results showed that compared with the model group, the increase of blood glucose was significantly inhibited in the yeast powder group; the MDA content in serum, liver and kidney was significantly decreased(P<0.05); the SOD activity was significantly increased(P<0.05). HE staining results showed that liver cells were neatly arranged in yeast powder group, steatosis was weakened, lymphocytes were decreased, glomerular hypertrophy was decreased and mesangial area was decreased, which indicating yeast powder could improve the antioxidant ability of liver and kidney, and alleviate the damage of liver and kidney caused by STZ and hyperglycemia.
The effects of Sporidiobolus pararoseus powder on liver and kidney injury of type 2 diabetic mice were studied, and the possible mechanism was explored. After C57 BL/6 mice were fed with high fat diet for 4 weeks, and injected intraperitoneally with streptozotocin(STZ) 100 mg/kg for 3 d,the model of type Ⅱ diabetes was established. The mice were divided into blank group, model group and yeast powder group(n=8). After continuous gastric perfusion for 8 weeks, the body weight, water intake, food intake, fasting blood glucose(FBG), superoxide dismutase(SOD) activity and malondialdehyde(MDA) content in serum, liver and kidney of mice were determined, and the pathological changes of liver and kidney were observed by hemotoxylin and eosin(HE) staining. The results showed that compared with the model group, the increase of blood glucose was significantly inhibited in the yeast powder group; the MDA content in serum, liver and kidney was significantly decreased(P<0.05); the SOD activity was significantly increased(P<0.05). HE staining results showed that liver cells were neatly arranged in yeast powder group, steatosis was weakened, lymphocytes were decreased, glomerular hypertrophy was decreased and mesangial area was decreased, which indicating yeast powder could improve the antioxidant ability of liver and kidney, and alleviate the damage of liver and kidney caused by STZ and hyperglycemia.
引文
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[12]ABDEL-RAHMAN H G,ABDELRAZEK H M A,ZEIDAN D W,et al.Lycopene:Hepatoprotective and antioxidant effects toward bisphenol A-induced toxicity in female wistar rats[J].Oxid Med Cell Long,2018,2018:https://doi.org/10.1155/2018/5167524.
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[14]MOLINE M,LIBKIND D,BROOCK M V.Production of torularhodin,torulene,and beta-carotene by Rhodotorula yeasts[J].Meth Mol Biol,2012,898:275-283.
[15]ERSHOV I V,DMITROVSKII A A,POLULIAKH O V,et al.Enzymatic conversion of torulene and torularhodine into retinal[J].Prikl Biokhim Mikrobiol,1992,28(5):680-684.
[16]GUO Y,LIU Y H,WANG Y X.Beneficial effect of lycopene on anti-diabetic nephropathy through diminishing inflammatory response and oxidative stress[J].Food Funct,2015,6(4):1150-1156.
[17]WANG X,XIAN T,JIA X,et al.A cross-sectional study on the associations of insulin resistance with sex hormone,abnormal lipid metabolism in T2DM and IGT patients[J].Medicine(Baltimore),2017,96(26):e7378.
[18]PARKINSON J,HAMREN B,KJELLSSON M C,et al.Application of the integrated glucose-insulin model for cross-study characterization of T2DM patients on metformin background treatment[J].Brit J Clin Pharmaco,2016,82(6):1613-1624.
[19]BAO L,LI J,ZHA D,et al.Chlorogenic acid prevents diabetic nephropathy by inhibiting oxidative stress and inflammation through modulation of the Nrf2/HO-1 and NF-k B pathways[J].Int Immunopharmacol,2018(54):245-253.
[20]YASSA H D,TOHAMY A F.Extract of Moringa oleifera leaves ameliorates streptozotocin-induced diabetes mellitus in adult rats[J].Acta Histochem,2014,116(5):844-854.
[21]WANG X,GU C S,HE W,et al.Glucose oxidase induces insulin resistance via influencing multiple targets in vitro and in vivo:The central role of oxidative stress[J].Biochimie,2012,94(8):1705-1717.
[22]AKASH M S,REHMAN K,CHEN S.Spice plant Allium cepa:dietary supplement for treatment of type 2 diabetes mellitus[J].Nutrition,2014,30(10):1128-1137.
[23]KARUNAKARAN U,PARK K G.A systematic review of oxidative stress and safety of antioxidants in diabetes:focus on islets and their defense[J].Diabetes Metab J,2013,37(2):106-112.
[24]STANCAKOVA A,PAANANEN J,SOININEN P,et al.Effects of 34risk loci for type 2 diabetes or hyperglycemia on lipoprotein subclasses and their composition in 6,580 nondiabetic finnish men[J].Diabetes,2011,60(5):1608-1616.
[25]YE G,METREVELI N S,DONTHI R V,et al.Catalase protects cardiomyocyte function in models of type 1 and type 2 diabetes[J].Diabetes,2004,53(5):1336-1343.
[2]KUCHAY M S,KRISHAN S,MISHRA S K,et al.Effect of empagliflozin on liver fat in patients with type 2 diabetes and nonalcoholic fatty liver disease:A randomized controlled trial(E-LIFT Trial)[J].Diabetes Care,2018,41(8):1801-1808.
[3]AHMADIEH H,AZAR S T.Liver disease and diabetes:Association,pathophysiology,and management[J].Diabetes Res Clin Pr,2014,104(1):53-62.
[4]CHOW F,OZOLS E,NIKOLIC-PATERSON D J,et al.Macrophages in mouse type 2 diabetic nephropathy:correlation with diabetic state and progressive renal injury[J].Kidney Int,2004,65(1):116-128.
[5]STOLAR M.Glycemic control and complications in type 2 diabetes mellitus[J].Am J Med,2010,123:S3-S11.
[6]ANJANEYULU M,CHOPRA K.Nordihydroguairetic acid,a lignin,prevents oxidative stress and the development of diabetic nephropathy in rats[J].Pharmacology,2004,72(1):42-50.
[7]BANDEIRA S D,DA FONSECA L J S,GUEDES G D,et al.Oxidative stress as an underlying contributor in the development of chronic complications in diabetes mellitus[J].Int J Mol Sci,2013,14(2):3265-3284.
[8]KASHIHARA N,HARUNA Y,KONDETI V K,et al.Oxidative stress in diabetic nephropathy[J].Curr Med Chem,2010,17(34):4256-4269.
[9]DU C,LI Y C,GUO Y H,et al.Torularhodin,isolated from Sporidiobolus pararoseus,inhibits human prostate cancer LNCa P and PC-3 cell growth through Bcl-2/Bax mediated apoptosis and AR down-regulation[J].Rsc Adv,2015,5(129):106387-106395.
[10]KOT A M,BLAZEJAK S,GIENTKA I,et al.Torulene and torularhodin:"new"fungal carotenoids for industry?[J].Microb Cell Fact,2018,17(1):49.
[11]韩梅,徐致远,钱和,等.锁掷酵母油中营养成分的分离和鉴定[J].微生物学通报,2016,43(1):60-68.
[12]ABDEL-RAHMAN H G,ABDELRAZEK H M A,ZEIDAN D W,et al.Lycopene:Hepatoprotective and antioxidant effects toward bisphenol A-induced toxicity in female wistar rats[J].Oxid Med Cell Long,2018,2018:https://doi.org/10.1155/2018/5167524.
[13]CHEW B P,PARK J S.Carotenoid action on the immune response[J].JNutr,2004,134(1):257s-261s.
[14]MOLINE M,LIBKIND D,BROOCK M V.Production of torularhodin,torulene,and beta-carotene by Rhodotorula yeasts[J].Meth Mol Biol,2012,898:275-283.
[15]ERSHOV I V,DMITROVSKII A A,POLULIAKH O V,et al.Enzymatic conversion of torulene and torularhodine into retinal[J].Prikl Biokhim Mikrobiol,1992,28(5):680-684.
[16]GUO Y,LIU Y H,WANG Y X.Beneficial effect of lycopene on anti-diabetic nephropathy through diminishing inflammatory response and oxidative stress[J].Food Funct,2015,6(4):1150-1156.
[17]WANG X,XIAN T,JIA X,et al.A cross-sectional study on the associations of insulin resistance with sex hormone,abnormal lipid metabolism in T2DM and IGT patients[J].Medicine(Baltimore),2017,96(26):e7378.
[18]PARKINSON J,HAMREN B,KJELLSSON M C,et al.Application of the integrated glucose-insulin model for cross-study characterization of T2DM patients on metformin background treatment[J].Brit J Clin Pharmaco,2016,82(6):1613-1624.
[19]BAO L,LI J,ZHA D,et al.Chlorogenic acid prevents diabetic nephropathy by inhibiting oxidative stress and inflammation through modulation of the Nrf2/HO-1 and NF-k B pathways[J].Int Immunopharmacol,2018(54):245-253.
[20]YASSA H D,TOHAMY A F.Extract of Moringa oleifera leaves ameliorates streptozotocin-induced diabetes mellitus in adult rats[J].Acta Histochem,2014,116(5):844-854.
[21]WANG X,GU C S,HE W,et al.Glucose oxidase induces insulin resistance via influencing multiple targets in vitro and in vivo:The central role of oxidative stress[J].Biochimie,2012,94(8):1705-1717.
[22]AKASH M S,REHMAN K,CHEN S.Spice plant Allium cepa:dietary supplement for treatment of type 2 diabetes mellitus[J].Nutrition,2014,30(10):1128-1137.
[23]KARUNAKARAN U,PARK K G.A systematic review of oxidative stress and safety of antioxidants in diabetes:focus on islets and their defense[J].Diabetes Metab J,2013,37(2):106-112.
[24]STANCAKOVA A,PAANANEN J,SOININEN P,et al.Effects of 34risk loci for type 2 diabetes or hyperglycemia on lipoprotein subclasses and their composition in 6,580 nondiabetic finnish men[J].Diabetes,2011,60(5):1608-1616.
[25]YE G,METREVELI N S,DONTHI R V,et al.Catalase protects cardiomyocyte function in models of type 1 and type 2 diabetes[J].Diabetes,2004,53(5):1336-1343.