养心颗粒对心力衰竭大鼠心肌功能的影响
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摘要
目的:观察养心颗粒对慢性心衰模型大鼠心脏超声心动图和血流动力学指标的影响。方法:采用腹腔注射阿霉素的方法复制心衰大鼠模型。6周末经超声心动图检测造模成功的大鼠随机分为模型对照组,养心颗粒组和依那普利组,每组12只,另取12只为正常对照组,各治疗组均给予药物灌胃4周。采用超声心动图检查左心室舒张末内径(LVEDD)、左心室收缩末内径(LVESD)、射血分数(LVEF)、左室短轴缩短率(LVFS);采用多导生理记录仪连续同步记录大鼠血流动力学变化。结果:与正常对照组比较,模型对照组、养心颗粒组、依那普利组大鼠LVEDD、LVESD、LVEDP显著升高(P<0.05),LVEF、LVFS、LVSP、±dp/dtmax显著降低(P<0005);与模型对照组相比,养心颗粒组、依那普利组大鼠LVEF、LVFS、LVSP、dp/dtmax明显升高(P<0.05),LVEDD、LVESD和LVEDP均明显降低(P<0.05);依那普利组与养心颗粒组比较无显著差异(P>0.05)。结论:养心颗粒可以显著增强心肌收缩力,改善左心室舒张功能,显著降低左心室前负荷,降低心肌耗氧量,从而改善心功能。
Objective: To observe the effects of Yangxin Granule on echocardiography and hemodynamic parameters in rats with chronic heart failure(CHF). Methods: CHF model was established by intraperitoneal injection of Doxorubicin. The succesful model was selected and randomly divided into three groups by echocardiography test after six weeks, i.e. the model group(n=12), Enalapril group(n=12), and Yangxin Granule group(n=12). Another 12 rats were taken as the blank control. The medication duration was four weeks. Left ventricular end diastolic diameter(LVEDD), left ventricular end systolic diameter(LVESD), left ventricular ejection fraction(LVEF), left ventricular fractional shortening(LVFS) were observed by echocardiography after four weeks of the treatment; hemodynamic changes were recorded by multi-channel physiological recorder. Results: After modeling LVEF,LVFS, and ±dp/dtmax were decreased significantly(P<0.05); LVESD, LVEDD and LVEDP increased significantly compared to those in the blank control group(P<0.05). LVEF,LVFS, LVSP and +dp/dtmax were increased significantly(P<0.05); LVEDD, LVESD and LVEDP were significantly decreased in Yangxin Granule group and in Enalapril group compared to those in the model group(P<0.05). There were no significant differences in above indexes between Enalapril group and Yangxin Granule group(P>0.05). Conclusion: Yangxin Granule can significantly strengthen myocardial contractility, improve left ventricular diastolic function, decrease lefe ventricular preload, reduce myocardial oxygen consumption, thus to improve cardial function.
Objective: To observe the effects of Yangxin Granule on echocardiography and hemodynamic parameters in rats with chronic heart failure(CHF). Methods: CHF model was established by intraperitoneal injection of Doxorubicin. The succesful model was selected and randomly divided into three groups by echocardiography test after six weeks, i.e. the model group(n=12), Enalapril group(n=12), and Yangxin Granule group(n=12). Another 12 rats were taken as the blank control. The medication duration was four weeks. Left ventricular end diastolic diameter(LVEDD), left ventricular end systolic diameter(LVESD), left ventricular ejection fraction(LVEF), left ventricular fractional shortening(LVFS) were observed by echocardiography after four weeks of the treatment; hemodynamic changes were recorded by multi-channel physiological recorder. Results: After modeling LVEF,LVFS, and ±dp/dtmax were decreased significantly(P<0.05); LVESD, LVEDD and LVEDP increased significantly compared to those in the blank control group(P<0.05). LVEF,LVFS, LVSP and +dp/dtmax were increased significantly(P<0.05); LVEDD, LVESD and LVEDP were significantly decreased in Yangxin Granule group and in Enalapril group compared to those in the model group(P<0.05). There were no significant differences in above indexes between Enalapril group and Yangxin Granule group(P>0.05). Conclusion: Yangxin Granule can significantly strengthen myocardial contractility, improve left ventricular diastolic function, decrease lefe ventricular preload, reduce myocardial oxygen consumption, thus to improve cardial function.
引文
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[13] 蔡琳.《2007 ESC心力衰竭和超声协会对诊断左室射血分数正常心力衰竭的共识》如何诊断舒张性心力衰竭[J].心血管病学进展,2008,29(1):17-20.
[14] 闫明昌,赵春芝,张东菊.慢性心力衰竭患者6 min步行试验、脑钠肽、左室舒张末期内径、左室射血分数的相关性研究[J].中国实用医药,2012,7(34):5-7.
[15] 张文亮.卡维地洛和美托洛尔对慢性充血性心力衰竭患者左室舒张功能及心室重塑的影响[J].中西医结合心脑血管病杂志,2012,10(2):235-236.
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[17] 沈蕾,王柯柯,杨景柯,等.心力衰竭治疗药物的表观遗传药理学进展[J].中国临床药理学与治疗学,2019,24(3):343-349.
[2] 曹剑.心力衰竭最新诊治进展[J].中华保健医学杂志,2017,19(5):375-378.
[3] 刘蓓蕾,吴钢.心脏疾病与能量代谢关系研究进展[J].疑难病杂志,2018,17(2):199-202.
[4] XING X Q,XU J,LU X W,et al.Combination of simvastain and losartan attenuates left ventricular myocardial fibrosis after pressure overload state in rats[J].Chin Pharmacol Bull,2009,25(10):1376-1379.
[5] REN Y L,XU J L,YU J,et al.Effect of benazepril and irbesartan on angiotensinⅡ receptor and ACE2 in chronic heart failure rats during cardiac remodeling[J].Chin Pharmacol Bull,2008,24(12):1582-1586.
[6] 汪晶晶,陈韵岱,智光.超声心动图评价左心室舒张功能进展[J].中华保健医学杂志,2013,15(1):73-75.
[7] 赵兰蒂,代世昌,杨建彬,等.心电图联合超声心动图检查对不同程度冠心病心力衰竭患者的临床诊断价值[J].临床误诊误治,2017,30(11):55-59.
[8] DE SIMONE G,DEVEREUX R B,CAMARGO M J,et al.Midwall left ventrieular performance in salt Loaded Dahl rats:effect of AT1 angiotensin II inhibition[J].J Hypertens,1995,13:12(Pt2):1808-1812.
[9] KAWANO H,CODY R J,GRAF K,et al.Angiotensin II enhances integrin and alpha-actinin expression in adult rat cardiac f-ibroblasts[J].Hypertension,2000,35(Pt 2):273-279.
[10] SCHWARZ E R,SPEAKMAN M T,KLONER R A.A new model of ventricular plication:a suturing technique to decrease left ventricular dimensions,improve contractility,and attenuate ventricular remodeling after myocardial infarction in the rat heart[J].J C ardiovasc Pharmacol,2000,5(1):41-49.
[11] 付治卿,刘涛,米亚非,等.超声心动图在评估犬慢性心力衰竭模型中的作用[J].心脏杂志,2007,19(5):528-530.
[12] 黄颖,李贻奎,孙明杰,等.高频率超声影像对冠脉结扎所致心力衰竭模型大鼠心功能动态观察[J].中国医药导报,2012,9(29):27-28.
[13] 蔡琳.《2007 ESC心力衰竭和超声协会对诊断左室射血分数正常心力衰竭的共识》如何诊断舒张性心力衰竭[J].心血管病学进展,2008,29(1):17-20.
[14] 闫明昌,赵春芝,张东菊.慢性心力衰竭患者6 min步行试验、脑钠肽、左室舒张末期内径、左室射血分数的相关性研究[J].中国实用医药,2012,7(34):5-7.
[15] 张文亮.卡维地洛和美托洛尔对慢性充血性心力衰竭患者左室舒张功能及心室重塑的影响[J].中西医结合心脑血管病杂志,2012,10(2):235-236.
[16] 苗连海,王峰.心力衰竭诊治进展[J].现代仪器与医疗,2018,24(2):14-16.
[17] 沈蕾,王柯柯,杨景柯,等.心力衰竭治疗药物的表观遗传药理学进展[J].中国临床药理学与治疗学,2019,24(3):343-349.