小麦条锈病菌致病类型G22-83及流行小种CYR32和CYR33在四川省小麦品种(系)上寄生适合度的测定及分析
|
摘要
为了解致病类型G22-83及流行小种CYR32和CYR33对四川省近年小麦生产品种和后备品种的寄生适合度,在田间分别将致病类型G22-83、流行小种CYR32和CYR33接种于四川省109个小麦品种(系),采用综合病情指数法测定3个供试菌株的相对寄生适合度。结果显示,致病类型G22-83、流行小种CYR32和CYR33的平均寄生适合度分别为0.1930、0.0560和0.0379,且前者显著高于后两者;流行小种CYR32和CYR33的平均相对寄生适合度有差异,但是差异不显著。致病类型G22-83、流行小种CYR32和CYR33在四川省109个小麦品种(系)上相对寄生适合度的中位数分别为0.0626、0.0000和0.0000,前者在四川省109个小麦品种(系)上相对寄生适合度的平均水平和波动程度均高于后两者。致病类型G22-83在四川省小麦生产品种和后备品种上的寄生适合度较高,在今后一段时间内可能成为该地区小麦条锈菌的优势菌系。
In order to know relative parasitic fitness of different races of Puccinia striiformis f. sp. tritici to wheat varieties in Sichuan Province, three races(CYR32, CYR33 and G22-83) of P. striiformis f.sp. tritici and 109 wheat cultivars(lines) collected from Sichuan Province were employed for a test of relative parasitic fitness. Relative parasitic fitness was calculated according to the observed values of epidemic components of different cultivar-race combinations(including infection type, prevalence and severity). Results showed that the values of average relative parasitic fitness of races CYR32, CYR33 and G22-83 were 0.0560, 0.0379 and 0.1930, respectively. The average relative parasitic fitness value of pathotype G22-83 was significantly higher than that of CYR32 and CYR33 at the population level. The average relative parasitic fitness values of CYR32 and CYR33 were different, but the difference was not significant. Box plot analysis was used to display the distribution of the relative parasitic fitness of three races in 109 wheat cultivars(lines). The results showed the median value of relative parasitic fit-ness of pathotype G22-83(0.0626) was higher than that of the predominant races CYR32(0.0000) and CYR33(0.0000). Due to its high parasitic fitness, pathotype G22 was becoming the predominant race in Sichuan Province.
In order to know relative parasitic fitness of different races of Puccinia striiformis f. sp. tritici to wheat varieties in Sichuan Province, three races(CYR32, CYR33 and G22-83) of P. striiformis f.sp. tritici and 109 wheat cultivars(lines) collected from Sichuan Province were employed for a test of relative parasitic fitness. Relative parasitic fitness was calculated according to the observed values of epidemic components of different cultivar-race combinations(including infection type, prevalence and severity). Results showed that the values of average relative parasitic fitness of races CYR32, CYR33 and G22-83 were 0.0560, 0.0379 and 0.1930, respectively. The average relative parasitic fitness value of pathotype G22-83 was significantly higher than that of CYR32 and CYR33 at the population level. The average relative parasitic fitness values of CYR32 and CYR33 were different, but the difference was not significant. Box plot analysis was used to display the distribution of the relative parasitic fitness of three races in 109 wheat cultivars(lines). The results showed the median value of relative parasitic fit-ness of pathotype G22-83(0.0626) was higher than that of the predominant races CYR32(0.0000) and CYR33(0.0000). Due to its high parasitic fitness, pathotype G22 was becoming the predominant race in Sichuan Province.
引文
Akhtar J.2007.Host-pathogen interaction and parasitic fitness of Phomopsis vexans to brinjal genotypes.Journal of Mycology&Plant Pathology,37(1):22-24
Alizon S,Michalakis Y.2015.Adaptive virulence evolution:the good old fitness-based approach.Trends in Ecology&Evolution,30(5):248-254
Bahri B,Kaltz O,Leconte M,de Vallavieille-Pope C,Enjalbert J.2009.Tracking costs of virulence in natural populations of the wheat pathogen,Puccinia striiformis f.sp.tritici.BMC Evolutionary Biology,9(1):26
Bai BB,Liu TG,Liu B,Gao L,Chen WQ.2017.High relative parasitic fitness of G22 derivatives is associated with the epidemic potential of wheat stripe rust in China.Plant Disease,https://doi.org/10.1094/PDIS-04-17-0511-SR
Barker JSF.2009.Defining fitness in natural and domesticated populations.//van der Werf J,Graser HU,Frankham R,Gondro C.Adaptation and fitness in animal populations:evolutionary and breeding perspectives on genetic resource management.Heidelberg:Springer,pp.3-14
Cao ZJ,Jing JX,Wang MN,Xu ZB,Shang HS,Li ZQ.2004.Analysis of stripe rust resistance inherence of wheat cultivar Guinong 22.Acta Botanica Boreali-Occidentalia Sinica,24(6):991-996(in Chinese)[曹张军,井金学,王美南,徐智斌,商鸿生,李振岐.2004.小麦品种贵农22号抗条锈基因遗传分析.西北植物学报,24(6):991-996]
Chen WQ,Kang ZS,Ma ZH,Xu SC,Jin SL,Jiang YY.2013.Integrated management of wheat stripe rust caused by Puccinia striiformis f.sp.tritici in China.Scientia Agricultura Sinica,46(20):4254-4262(in Chinese)[陈万权,康振生,马占鸿,徐世昌,金社林,姜玉英.2013.中国小麦条锈病综合治理理论与实践.中国农业科学,46(20):4254-4262]
Dobzhansky T.1955.A review of some fundamental concepts and problems of population genetics.Cold Spring Harbor Symposia on Quantitative Biology,20:1-15
Feng F,Zeng SH.1990.The relationship between infection type and spore yield of wheat stripe rust.Acta Agriculturae Universitatis Pekinensis,16(S):174-179(in Chinese)[冯锋,曾士迈.1990.小麦条锈病反应型和产孢量关系的初步研究I.成株期试验.北京农业大学学报,16(S):174-179]
Fraile A,Pagán I,Anastasio G,Sáez E,García-Arenal F.2011.Rapid genetic diversification and high fitness penalties associated with pathogenicity evolution in a plant virus.Molecular Biology&Evolution,28(4):1425-1437
Jia QZ,Jin SL,Cao SQ,Luo HS,Jin MA.2007.Tending to prevalence and progress of CY32 and Shuiyuan14 pathotypes in Gansu Province.Journal of Plant Protection,34(3):263-267(in Chinese)[贾秋珍,金社林,曹世勤,骆惠生,金明安.2007.小麦条锈菌生理小种条中32号及水源14致病类型在甘肃的流行与发展趋势.植物保护学报,34(3):263-267]
Kirchner JW,Roy BA.2002.Evolutionary implications of host-pathogen specificity:fitness consequences of pathogen virulence traits.Evolutionary Ecology Research,4:27-48
Li Q,He MM,Dong HL,Yao Q,Jing JX,Wang BT.2011.Genetic analysis and molecular mapping for stripe rust resistance gene(s)in wheat cultivar Guinong22.Acta Phytopathologica Sinica,41(5):495-501(in Chinese)[李强,贺苗苗,董海丽,姚强,井金学,王保通.2011.小麦品种贵农22抗条锈基因的遗传分析及分子标记.植物病理学报,41(5):495-501]
Li ZQ,Zeng SM.2002.Wheat rusts in China.Beijing:China Agriculture Press(in Chinese)[李振岐,曾士迈.2002.中国小麦锈病.北京:中国农业出版社]
Salvaudon L,Héraudet V,Shykoff JA.2007.Genotype-specific interactions and the trade-off between host and parasite fitness.BMCEvolutionary Biology,7(1):189
Wan AM,Zhao ZH,Chen XM,He ZH,Jin SL,Jia QZ,Yao G,Yang JX,Wang BT,Li GB,et al.2004.Wheat stripe rust epidemic and virulence of Puccinia striiformis f.sp tritici in China in2002.Plant Disease,88(8):896-904
Wang BT,Li GB,Li Q,Wang F,Shi YQ,Liu QR,Kang ZS.2009.Parasitic fitness of the major epidemic strains of Puccinia striiformis f.sp.tritici in China.Acta Phytopathologica Sinica,39(1):82-87(in Chinese)[王保通,李高宝,李强,王芳,史亚千,刘倩如,康振生.2009.中国小麦条锈菌主要流行菌系的寄生适合度研究.植物病理学报,39(1):82-87]
Xiao YY,Wu LR,Hu JH,Wan AM,Chang W.2007.Forecast of race dynamic of wheat stripe rust.Journal of Plant Protection,34(3):257-262(in Chinese)[肖悦岩,吴立人,胡家怀,万安民,常稳.2007.小麦条锈菌生理小种动态预测.植物保护学报,34(3):257-262
Zeng SM.1996.Study on the method for estimation the parasitic fitness of plant pathogenic fungi,exemplified with Puccinia striiformis West.Acta Phytopathologica Sinica,26(2):97-104(in Chinese)[曾士迈.1996.植物病原菌寄生适合度测定方法的研究(以小麦条锈菌为例).植物病理学报,26(2):97-104]
Zeng SM,Luo Y.2006.Long-distance spread and interregional epidemics of wheat stripe rust in China.Plant Disease,90(8):980-988
Zeng SM,Wang PY.1990.Preliminary study on the method of estimation of parasitic fitness in the cultivar-race combinations of wheat stripe rust.Acta Agriculturae Universitatis Pekinensis,16(Supplement):163-169(in Chinese)[曾士迈,王沛有.1990.寄生适合度测定方法的初步研究.北京农业大学学报,16(增):163-169]
Zeng SM,Zhang SZ.1998.Epidemiological analysis of plant breeding for disease resistance.Beijing:Science Press,pp.41-42(in Chinese)[曾士迈,张树榛.1998.植物抗病育种的流行学研究.北京:科学出版社,pp.41-42]
Zhang CF,Shang HS,Li ZQ.1994.Parasitic fitness of the major epidemic races of wheat stripe rust in China.Acta Universitatis Agriculturalis Boreali-Occidentalis,22(2):28-32(in Chinese)[张传飞,商鸿生,李振岐.1994.中国小麦条锈菌主要流行小种的寄生适合度.西北农业大学学报,22(2):28-32]
Alizon S,Michalakis Y.2015.Adaptive virulence evolution:the good old fitness-based approach.Trends in Ecology&Evolution,30(5):248-254
Bahri B,Kaltz O,Leconte M,de Vallavieille-Pope C,Enjalbert J.2009.Tracking costs of virulence in natural populations of the wheat pathogen,Puccinia striiformis f.sp.tritici.BMC Evolutionary Biology,9(1):26
Bai BB,Liu TG,Liu B,Gao L,Chen WQ.2017.High relative parasitic fitness of G22 derivatives is associated with the epidemic potential of wheat stripe rust in China.Plant Disease,https://doi.org/10.1094/PDIS-04-17-0511-SR
Barker JSF.2009.Defining fitness in natural and domesticated populations.//van der Werf J,Graser HU,Frankham R,Gondro C.Adaptation and fitness in animal populations:evolutionary and breeding perspectives on genetic resource management.Heidelberg:Springer,pp.3-14
Cao ZJ,Jing JX,Wang MN,Xu ZB,Shang HS,Li ZQ.2004.Analysis of stripe rust resistance inherence of wheat cultivar Guinong 22.Acta Botanica Boreali-Occidentalia Sinica,24(6):991-996(in Chinese)[曹张军,井金学,王美南,徐智斌,商鸿生,李振岐.2004.小麦品种贵农22号抗条锈基因遗传分析.西北植物学报,24(6):991-996]
Chen WQ,Kang ZS,Ma ZH,Xu SC,Jin SL,Jiang YY.2013.Integrated management of wheat stripe rust caused by Puccinia striiformis f.sp.tritici in China.Scientia Agricultura Sinica,46(20):4254-4262(in Chinese)[陈万权,康振生,马占鸿,徐世昌,金社林,姜玉英.2013.中国小麦条锈病综合治理理论与实践.中国农业科学,46(20):4254-4262]
Dobzhansky T.1955.A review of some fundamental concepts and problems of population genetics.Cold Spring Harbor Symposia on Quantitative Biology,20:1-15
Feng F,Zeng SH.1990.The relationship between infection type and spore yield of wheat stripe rust.Acta Agriculturae Universitatis Pekinensis,16(S):174-179(in Chinese)[冯锋,曾士迈.1990.小麦条锈病反应型和产孢量关系的初步研究I.成株期试验.北京农业大学学报,16(S):174-179]
Fraile A,Pagán I,Anastasio G,Sáez E,García-Arenal F.2011.Rapid genetic diversification and high fitness penalties associated with pathogenicity evolution in a plant virus.Molecular Biology&Evolution,28(4):1425-1437
Jia QZ,Jin SL,Cao SQ,Luo HS,Jin MA.2007.Tending to prevalence and progress of CY32 and Shuiyuan14 pathotypes in Gansu Province.Journal of Plant Protection,34(3):263-267(in Chinese)[贾秋珍,金社林,曹世勤,骆惠生,金明安.2007.小麦条锈菌生理小种条中32号及水源14致病类型在甘肃的流行与发展趋势.植物保护学报,34(3):263-267]
Kirchner JW,Roy BA.2002.Evolutionary implications of host-pathogen specificity:fitness consequences of pathogen virulence traits.Evolutionary Ecology Research,4:27-48
Li Q,He MM,Dong HL,Yao Q,Jing JX,Wang BT.2011.Genetic analysis and molecular mapping for stripe rust resistance gene(s)in wheat cultivar Guinong22.Acta Phytopathologica Sinica,41(5):495-501(in Chinese)[李强,贺苗苗,董海丽,姚强,井金学,王保通.2011.小麦品种贵农22抗条锈基因的遗传分析及分子标记.植物病理学报,41(5):495-501]
Li ZQ,Zeng SM.2002.Wheat rusts in China.Beijing:China Agriculture Press(in Chinese)[李振岐,曾士迈.2002.中国小麦锈病.北京:中国农业出版社]
Salvaudon L,Héraudet V,Shykoff JA.2007.Genotype-specific interactions and the trade-off between host and parasite fitness.BMCEvolutionary Biology,7(1):189
Wan AM,Zhao ZH,Chen XM,He ZH,Jin SL,Jia QZ,Yao G,Yang JX,Wang BT,Li GB,et al.2004.Wheat stripe rust epidemic and virulence of Puccinia striiformis f.sp tritici in China in2002.Plant Disease,88(8):896-904
Wang BT,Li GB,Li Q,Wang F,Shi YQ,Liu QR,Kang ZS.2009.Parasitic fitness of the major epidemic strains of Puccinia striiformis f.sp.tritici in China.Acta Phytopathologica Sinica,39(1):82-87(in Chinese)[王保通,李高宝,李强,王芳,史亚千,刘倩如,康振生.2009.中国小麦条锈菌主要流行菌系的寄生适合度研究.植物病理学报,39(1):82-87]
Xiao YY,Wu LR,Hu JH,Wan AM,Chang W.2007.Forecast of race dynamic of wheat stripe rust.Journal of Plant Protection,34(3):257-262(in Chinese)[肖悦岩,吴立人,胡家怀,万安民,常稳.2007.小麦条锈菌生理小种动态预测.植物保护学报,34(3):257-262
Zeng SM.1996.Study on the method for estimation the parasitic fitness of plant pathogenic fungi,exemplified with Puccinia striiformis West.Acta Phytopathologica Sinica,26(2):97-104(in Chinese)[曾士迈.1996.植物病原菌寄生适合度测定方法的研究(以小麦条锈菌为例).植物病理学报,26(2):97-104]
Zeng SM,Luo Y.2006.Long-distance spread and interregional epidemics of wheat stripe rust in China.Plant Disease,90(8):980-988
Zeng SM,Wang PY.1990.Preliminary study on the method of estimation of parasitic fitness in the cultivar-race combinations of wheat stripe rust.Acta Agriculturae Universitatis Pekinensis,16(Supplement):163-169(in Chinese)[曾士迈,王沛有.1990.寄生适合度测定方法的初步研究.北京农业大学学报,16(增):163-169]
Zeng SM,Zhang SZ.1998.Epidemiological analysis of plant breeding for disease resistance.Beijing:Science Press,pp.41-42(in Chinese)[曾士迈,张树榛.1998.植物抗病育种的流行学研究.北京:科学出版社,pp.41-42]
Zhang CF,Shang HS,Li ZQ.1994.Parasitic fitness of the major epidemic races of wheat stripe rust in China.Acta Universitatis Agriculturalis Boreali-Occidentalis,22(2):28-32(in Chinese)[张传飞,商鸿生,李振岐.1994.中国小麦条锈菌主要流行小种的寄生适合度.西北农业大学学报,22(2):28-32]
© 2004-2018 中国地质图书馆版权所有 京ICP备05064691号 京公网安备11010802017129号 地址：北京市海淀区学院路29号 邮编：100083 电话：办公室：(+86 10)66554848；文献借阅、咨询服务、科技查新：66554700 |