正交试验优化山地五月茶总黄酮的提取工艺
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摘要
通过单因素试验,分析乙醇浓度、提取温度、提取时间、料液比、提取次数等对总黄酮提取率的影响,采用正交试验确定山地五月茶总黄酮的最佳提取工艺。结果表明,山地五月茶总黄酮的最佳提取条件为乙醇浓度85%、料液质量体积比1∶10、提取温度90℃,加热回流提取2.0 h,提取3次。在此条件下,测得山地五月茶总黄酮的含量为(4.548 6±0.041 5)mg/g。本研究建立了山地五月茶总黄酮的提取工艺,可为山地五月茶资源的充分利用、研究开发等提供科学依据。
Total flavonoids were extracted from Antidesma montanum by using single-factor experiment to determine the effect of ethanol concentration, temperature, duration of sample extraction, solid-liquid ratio and extraction times on the extraction ratio of total flavonoids.An orthogonal experimental design was arranged to optimize the technology for extraction of the total flavonoids from A.montanum.The results showed that the optimal condition for extracting total flavonoids from A.montanum was as follows:5%ethanol, solid-liquid ratio of 1∶10, temperature 90℃, and heat reflux extraction for 2 h and 3 times.Under the optimal condition, the total flavonoids content extracted from A.montanum was(4.548 6±0.041 5) mg/g.An optimal technology for extraction of total flavonoids from A.montanum was hence established, which would provide scientific data for further research, development and utilization of A.montanum germplasm resources.
Total flavonoids were extracted from Antidesma montanum by using single-factor experiment to determine the effect of ethanol concentration, temperature, duration of sample extraction, solid-liquid ratio and extraction times on the extraction ratio of total flavonoids.An orthogonal experimental design was arranged to optimize the technology for extraction of the total flavonoids from A.montanum.The results showed that the optimal condition for extracting total flavonoids from A.montanum was as follows:5%ethanol, solid-liquid ratio of 1∶10, temperature 90℃, and heat reflux extraction for 2 h and 3 times.Under the optimal condition, the total flavonoids content extracted from A.montanum was(4.548 6±0.041 5) mg/g.An optimal technology for extraction of total flavonoids from A.montanum was hence established, which would provide scientific data for further research, development and utilization of A.montanum germplasm resources.
引文
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[3]陈园.正交试验优化紫荆果总黄酮提取及其抗氧化性[J].山东农业大学学报(自然科学版),2016,47(1):43-46.
[4]云南省药材公司.云南中药资源名录[M].北京:科学出版社,1993:277.
[5]周丹,艾朝辉,韩丽.山地五月茶挥发油的化学成分分析[J].时珍国医国药,2012,23(1):65-66.
[6]周丹,韩丽,林连波,等.山地五月茶化学成分预试研究[J].中国医药导报,2011,8(13):37-38.
[7]韩丽,刘月丽,王小蒙,等.山地五月茶急性毒性及抗炎作用试验研究[J].时珍国医国药,2012,23(6):1 391-1 393.
[8]周丹,张万科,赖伟勇,等.山地五月茶微量元素含量的测定[J].海南医学院学报,2012,18(3):308-309.
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