月季F_1代群体表型性状变异分析
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摘要
以月季(Rosa spp.)品种‘云蒸霞蔚’和‘太阳城’正交获得的184株F_1代群体为研究对象,采用方差分析、变异系数、遗传分析、相关性分析对花部形态性状以及叶片形态性状进行测定分析。结果表明:该杂交群体表型变异丰富,变异系数在7.33%～68.08%,其中花瓣数量在群体中的变异程度最高;杂交后代各个性状上均发生分离变异,出现不同于亲本的表现型,且离散程度较高。表型性状的遗传特点与关联分析,为挖掘控制表型性状的优良基因及辅助选择育种提供参考。
Heredity of some characters of flower and leaf in F_1 individuals of mother(Rosa 'Yunzheng Xiawei') and father(R.'Sun City') were measured and analyzed with variance,coefficient of variation,inheritance and correlation.The coefficient of variation in the hybrid population was 7.33%-68.08%,indicating a significant phenotypic variation.Among them,the number of petals in the population showed the highest degree of variation.All the traits measured had high degree of dispersion and came out new phenotypic traits,different from parents.The analysis of the genetic characteristics and correlation of phenotypic traits will benefit for selecting breeding and assisting breeding for dominant genes in controlling phenotypic traits.
Heredity of some characters of flower and leaf in F_1 individuals of mother(Rosa 'Yunzheng Xiawei') and father(R.'Sun City') were measured and analyzed with variance,coefficient of variation,inheritance and correlation.The coefficient of variation in the hybrid population was 7.33%-68.08%,indicating a significant phenotypic variation.Among them,the number of petals in the population showed the highest degree of variation.All the traits measured had high degree of dispersion and came out new phenotypic traits,different from parents.The analysis of the genetic characteristics and correlation of phenotypic traits will benefit for selecting breeding and assisting breeding for dominant genes in controlling phenotypic traits.
引文
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16.文自翔,赵团结,郑永战,等.中国栽培和野生大豆农艺品质性状与SSR标记的关联分析Ⅰ.群体结构及关联标记[J].作物学报,2008,34(7):1169-1178.Wen Z X,Zhao T J,Zheng Y Z,et al.Association analysis of agronomic and quality traits with SSR markers in Glycine max and Glycine soja in China:Ⅰ.Population structure and associated markers[J].Acta Agronomica Sinica,2008,34(7):1169-1178.
17.郭宁.新疆天山山脉地区疏花蔷薇与宽刺蔷薇天然群体遗传多样性分析[D].北京:中国农业科学院,2010.Guo N.Genetic diversity of natural populations of Rosa laxa Retz.and Rosa platyacantha Schrenk in Tianshan Mountains of Xinjiang[D].Beijing:Chinese Academy of Agricultural Sciences,2010.
18.李宗艳,张海燕.黄牡丹表型变异及多样性研究[J].西北林学院学报,2011,26(4):117-122.Li Z Y,Zhang H Y.Morphological variation and diversity in populations of Paeonia lutea[J].Journal of Northwest Forestry University,2011,26(4):117-122.
19.贺丹,唐婉,刘阳,等.尾叶紫薇与紫薇F1代群体主要表型性状与SSR标记的连锁分析[J].北京林业大学学报,2012,34(6):121-125.He D,Tang W,Liu Y,et al.Linkage analysis of phonotypic traits of Lagerstroemia caudata and L.indica F1population using SSR markers[J].Journal of Beijing Forestry University,2012,34(6):121-125.
20.周俐宏,王金刚,王志刚,等.现代月季6个表型性状和SSR标记的关联分析[J].辽宁农业科学,2017(3):9-14.Zhou L H,Wang J G,Wang Z G,et al.Association analysis of six phenotype traits and SSR markers in modern roses[J].Liaoning Agricultural Sciences,2017(3):9-14.
21.崔虎亮,黄弄璋,闫海川,等.油用牡丹单株产量和主要表型性状的相关性[J].华南农业大学学报,2017,38(2):86-91.Cui H L,Huang N Z,Yan H C,et al.Correlation between yield per plant and main phenotypic traits of Paeonia suffruticosa for oil production[J].Journal of South China Agricultural University,2017,38(2):86-91.
22.徐斌,彭莉霞,杨会肖,等.杜鹃红山茶叶片主要性状的遗传多样性分析[J].植物研究,2015,35(5):730-734.Xu B,Peng L X,Yang H X,et al.Genetic diversity analysis for leaf main traits of Camellia azalea[J].Bulletin of Botanical Research,2015,35(5):730-734.
23.宿庆连.回归方程法测定红掌叶面积研究[J].广东农业科学,2009,(1):57-59.Su Q L.Anthurium andraeanum study on measuring leaf area[J].Guangdong Agricultural Sciences,2009,(1):57-59.
24.杜铃,王华新,廖美兰,等.杜鹃红山茶叶面积回归方程的建立[J].广西林业科学,2014,43(3):312-315.Du L,Wang H X,Liao M L,et al.Establishment of regressive equation for leaf area of Camellia azalea[J].Guangxi Forestry Science,2014,43(3):312-315.
25.赵印泉,刘青林.重瓣花的形成机理及遗传特性研究进展[J].西北植物学报,2009,29(4):832-841.Zhao Y Q,Liu Q L.Research advances in the formation mechanism and genetic characters of double flowers[J].Acta Botanica Boreali-Occidentalia Sinica,2009,29(4):832-841.
26.Serrato C M A.Contribution to the study of marigold(Tagetes sp.)flower traits[J].Revista Chapingo,1990,15(71-72):151-155.
27.黄秀强,陈俊愉,黄国振.莲属两个种亲缘关系的初步研究[J].园艺学报,1992,19(2):164-170.Huang X Q,Chen J Y,Huang G Z.Preliminary studies on biosytematic relationship between the two Nelumbo species[J].Acta Horticulturae Sinica,1992,19(2):164-170.
2.许凤,张颢,李凌.月季杂交亲和性研究进展[J].安徽农学通报(下半月刊),2009,15(8):143-145.Xu F,Zhang H,Li L.Advances in research of cross compatibility of rose[J].Anhui Agricultural Science Bulletin,2009,15(8):143-145.
3.张佐双,朱秀珍.中国月季[M].北京:中国林业出版社,2006:220.Zhang Z S,Zhu X Z.Rose of China[M].Beijing:China Forestry Publishing House,2006:220.
4.贺蕤,杨希,刘青林.月季育种的国内现状和国际趋势[J].中国园林,2017,33(12):35-41.He R,Yang X,Liu Q L.Domestic status and international trend for rose breeding[J].Chinese Landscape Architecture,2017,33(12):35-41.
5.白锦荣.部分蔷薇属种质资源亲缘关系分析及抗白粉病育种[D].北京:北京林业大学,2009.Bai J R.Analysis of genetic relationships between wild species and Chinese traditional roses and powdery mildew resistance breeding[D].Beijing:Beijing Forestry University,2009.
6.李智.月季远缘杂交研究(Ⅵ)[D].北京:北京林业大学,2015.Li Z.Studies on wide cross breeding of roses(Ⅵ)[D].Beijing:Beijing Forestry University,2015.
7.丁榕,崔金腾,袁园,等.月季遗传多样性和杂交亲本的选择[J].北京农学院学报,2015,30(2):72-77.Ding R,Cui J T,Yuan Y,et al.Rose genetic diversity and selection of hybrid parents[J].Journal of Beijing University of Agriculture,2015,30(2):72-77.
8.戴思兰.园林植物遗传学:2版[M].北京:中国林业出版社,2010:297.Dai S L.Genetics of landscape plants:2nd ed[M].Beijing:China Forestry Publishing House,2010:297.
9.杨云燕,温超,王珂永,等.切花菊杂交F1代若干性状的遗传分析[J].中国农业大学学报,2015,20(5):179-187.Yang Y Y,Wen C,Wang K Y,et al.Heredity analysis of several characters in F1hybrid generation of cut-flower chrysanthemums[J].Journal of China Agricultural University,2015,20(5):179-187.
10.Debener T,Linde M.Exploring complex ornamental genomes:the rose as a model plant[J].Critical Reviews in Plant Sciences,2009,28(4):267-280.
11.Melchinger A E,Utz H F,Sch9n C C.QTL analyses of complex traits with cross validation,bootstrapping and other biometric methods[J].Euphytica,2004,137(1):1-11.
12.Ruan L L,Gao Y K,Wu Q,et al.An analysis of the genetic variation in ornamental traits in hybrids of Iris dichotoma and I.domestica[J].Euphytica,2017,213:8.
13.车代弟,张晓莹,张金柱,等.蔷薇属植物数量性状位点定位的研究进展[J].园艺学报,2016,43(9):1765-1775.Che D D,Zhang X Y,Zhang J Z,et al.A review of the quantitative trait loci in Rosa[J].Acta Horticulturae Sinica,2016,43(9):1765-1775.
14.张恒敢,杨四军,顾克军,等.应用数字图像处理测定作物叶面积的简便方法[J].江苏农业科学,2002(3):20-21,25.Zhang H G,Yang S J,Gu K J,et al.A simple method of measuring crop leaf area by digital image processing[J].Jiangsu Agricultural Sciences,2002(3):20-21,25.
15.程金水,刘青林.园林植物遗传育种学:2版[M].北京:中国林业出版社,2010:10,520,16.Cheng J S,Liu Q L.Genetics and breeding of landscape plants:2nd ed[M].Beijing:China Forestry Publishing House,2010:10,520,16.
16.文自翔,赵团结,郑永战,等.中国栽培和野生大豆农艺品质性状与SSR标记的关联分析Ⅰ.群体结构及关联标记[J].作物学报,2008,34(7):1169-1178.Wen Z X,Zhao T J,Zheng Y Z,et al.Association analysis of agronomic and quality traits with SSR markers in Glycine max and Glycine soja in China:Ⅰ.Population structure and associated markers[J].Acta Agronomica Sinica,2008,34(7):1169-1178.
17.郭宁.新疆天山山脉地区疏花蔷薇与宽刺蔷薇天然群体遗传多样性分析[D].北京:中国农业科学院,2010.Guo N.Genetic diversity of natural populations of Rosa laxa Retz.and Rosa platyacantha Schrenk in Tianshan Mountains of Xinjiang[D].Beijing:Chinese Academy of Agricultural Sciences,2010.
18.李宗艳,张海燕.黄牡丹表型变异及多样性研究[J].西北林学院学报,2011,26(4):117-122.Li Z Y,Zhang H Y.Morphological variation and diversity in populations of Paeonia lutea[J].Journal of Northwest Forestry University,2011,26(4):117-122.
19.贺丹,唐婉,刘阳,等.尾叶紫薇与紫薇F1代群体主要表型性状与SSR标记的连锁分析[J].北京林业大学学报,2012,34(6):121-125.He D,Tang W,Liu Y,et al.Linkage analysis of phonotypic traits of Lagerstroemia caudata and L.indica F1population using SSR markers[J].Journal of Beijing Forestry University,2012,34(6):121-125.
20.周俐宏,王金刚,王志刚,等.现代月季6个表型性状和SSR标记的关联分析[J].辽宁农业科学,2017(3):9-14.Zhou L H,Wang J G,Wang Z G,et al.Association analysis of six phenotype traits and SSR markers in modern roses[J].Liaoning Agricultural Sciences,2017(3):9-14.
21.崔虎亮,黄弄璋,闫海川,等.油用牡丹单株产量和主要表型性状的相关性[J].华南农业大学学报,2017,38(2):86-91.Cui H L,Huang N Z,Yan H C,et al.Correlation between yield per plant and main phenotypic traits of Paeonia suffruticosa for oil production[J].Journal of South China Agricultural University,2017,38(2):86-91.
22.徐斌,彭莉霞,杨会肖,等.杜鹃红山茶叶片主要性状的遗传多样性分析[J].植物研究,2015,35(5):730-734.Xu B,Peng L X,Yang H X,et al.Genetic diversity analysis for leaf main traits of Camellia azalea[J].Bulletin of Botanical Research,2015,35(5):730-734.
23.宿庆连.回归方程法测定红掌叶面积研究[J].广东农业科学,2009,(1):57-59.Su Q L.Anthurium andraeanum study on measuring leaf area[J].Guangdong Agricultural Sciences,2009,(1):57-59.
24.杜铃,王华新,廖美兰,等.杜鹃红山茶叶面积回归方程的建立[J].广西林业科学,2014,43(3):312-315.Du L,Wang H X,Liao M L,et al.Establishment of regressive equation for leaf area of Camellia azalea[J].Guangxi Forestry Science,2014,43(3):312-315.
25.赵印泉,刘青林.重瓣花的形成机理及遗传特性研究进展[J].西北植物学报,2009,29(4):832-841.Zhao Y Q,Liu Q L.Research advances in the formation mechanism and genetic characters of double flowers[J].Acta Botanica Boreali-Occidentalia Sinica,2009,29(4):832-841.
26.Serrato C M A.Contribution to the study of marigold(Tagetes sp.)flower traits[J].Revista Chapingo,1990,15(71-72):151-155.
27.黄秀强,陈俊愉,黄国振.莲属两个种亲缘关系的初步研究[J].园艺学报,1992,19(2):164-170.Huang X Q,Chen J Y,Huang G Z.Preliminary studies on biosytematic relationship between the two Nelumbo species[J].Acta Horticulturae Sinica,1992,19(2):164-170.