体内和体外评价法分析川秋葵微粉的抗氧化活性
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摘要
研究川秋葵微粉的体内和体外的抗氧化活性及可能的作用机制。将50只小鼠随机分为对照组、螺旋藻粉阳性药物组以及川秋葵微粉低、中、高剂量组(450、900、1800 mg/kg)。连续灌胃给药6周,末次灌胃后禁食12 h,颈椎脱臼处死后小鼠眼球取血,即刻取出肝脏组织和脑组织并测定相关指标。体外抗氧化功能评价采用体外·OH清除率、·O2—清除率、抑制脂质过氧化能力、ABTS+·清除率、DPPH·清除率、还原力、总抗氧化能力7个的指标。结果表明:川秋葵微粉具有良好的体外抗氧化活性,对DPPH·、·OH、ABTS+·的清除能力优于商品化的螺旋藻微粉。川秋葵微粉具有良好的体内抗氧化活性,与对照组相比,经高剂量川秋葵微粉干预后能够显著降低小鼠血清(p<0.01)、脑和肝脏组织(p<0.05)中MDA含量;提高小鼠血清(p>0.05)、脑组织(p<0.01)和肝脏组织(p<0.05)中SOD活性;提高小鼠血清(p<0.05)、脑组织(p<0.01)和肝脏组织(p>0.05)中GSH-Px活性;提高小鼠血清(p<0.01)、脑组织(p<0.01)和肝脏组织(p>0.05)中CAT活性;提高小鼠血清(p<0.01)、脑组织(p<0.01)和肝脏组织(p<0.05)中T-AOC能力。以上结果提示:川秋葵微粉能够提高机体的抗氧化能力,作用机制可能与清除自由基,抑制脂质过氧化及维持机体抗氧化酶系活性的正常进行有关;高剂量川秋葵微粉对脑组织和血清的抗氧化能力优于肝脏组织;高剂量川秋葵微粉对小鼠机体的抗氧化能力与阳性对照组(螺旋藻)相当。
Objective to study the antioxidant activity and possible mechanism of Sichuan okra powder in vivo and in vitro. 50 mice were randomly divided into control group, spirulina powder positive drug group and low, medium and high dose group of okra micropowder(450, 900, 1800 mg/kg). After 6 weeks of continuous perfusion of the stomach, 12 h was fasted after the last gastric perfusion. After the dislocation of the cervical vertebra, the blood was taken from the eyeball of the mice. The liver tissue and brain tissue were taken out immediately and the related indexes were measured. In vitro antioxidant function was evaluated by 7 indexes, namely, ·OH clearance rate, ·O2— clearance rate, inhibition of lipid peroxidation, ABTS+· clearance rate, DPPH clearance rate, reducing power and total antioxidant activity. The results showed that the okra micropowder had good antioxidant activity in vitro, and its scavenging capacity for DPPH, ·OH, ABTS+ was better than that of commercialized spirulina powder. Okra powder has good antioxidant activity in vivo. Compared with the control group, the MDA content in serum(P<0.01), brain and liver tissue(P<0.05) was significantly reduced after the intervention of high dose of okra micropowder, and the activity of SOD in mice serum(P>0.05), brain tissue(P<0.01) and liver tissue(P<0.05) was increased. The activity of GSH-Px in mice serum(P<0.05), brain tissue(P<0.01) and liver tissue(P>0.05) was improved, and the activity of CAT in mice serum(P<0.01), brain tissue(P<0.01) and liver tissue(P>0.05) was improved. The T-AOC ability of mice serum(P<0.01), brain tissue(P<0.01) and liver tissue(P<0.05) was increased. These results suggested that the micropowder of okra could improve the antioxidant capacity of the body. The mechanism of action might be related to the removal of free radicals, the inhibition of lipid peroxidation and the maintenance of the activity of antioxidant enzyme system. The antioxidant capacity of high dose of okra micropowder on brain tissue and serum was better than that of liver tissue, and the antioxidant capacity of high dose of okra micro powder was equivalent to that of positive control group(Spirulina).
Objective to study the antioxidant activity and possible mechanism of Sichuan okra powder in vivo and in vitro. 50 mice were randomly divided into control group, spirulina powder positive drug group and low, medium and high dose group of okra micropowder(450, 900, 1800 mg/kg). After 6 weeks of continuous perfusion of the stomach, 12 h was fasted after the last gastric perfusion. After the dislocation of the cervical vertebra, the blood was taken from the eyeball of the mice. The liver tissue and brain tissue were taken out immediately and the related indexes were measured. In vitro antioxidant function was evaluated by 7 indexes, namely, ·OH clearance rate, ·O2— clearance rate, inhibition of lipid peroxidation, ABTS+· clearance rate, DPPH clearance rate, reducing power and total antioxidant activity. The results showed that the okra micropowder had good antioxidant activity in vitro, and its scavenging capacity for DPPH, ·OH, ABTS+ was better than that of commercialized spirulina powder. Okra powder has good antioxidant activity in vivo. Compared with the control group, the MDA content in serum(P<0.01), brain and liver tissue(P<0.05) was significantly reduced after the intervention of high dose of okra micropowder, and the activity of SOD in mice serum(P>0.05), brain tissue(P<0.01) and liver tissue(P<0.05) was increased. The activity of GSH-Px in mice serum(P<0.05), brain tissue(P<0.01) and liver tissue(P>0.05) was improved, and the activity of CAT in mice serum(P<0.01), brain tissue(P<0.01) and liver tissue(P>0.05) was improved. The T-AOC ability of mice serum(P<0.01), brain tissue(P<0.01) and liver tissue(P<0.05) was increased. These results suggested that the micropowder of okra could improve the antioxidant capacity of the body. The mechanism of action might be related to the removal of free radicals, the inhibition of lipid peroxidation and the maintenance of the activity of antioxidant enzyme system. The antioxidant capacity of high dose of okra micropowder on brain tissue and serum was better than that of liver tissue, and the antioxidant capacity of high dose of okra micro powder was equivalent to that of positive control group(Spirulina).
引文
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[19]任丹丹.黄秋葵多糖提取纯化及其体外结合胆酸能力和抑制肿瘤活性分析[D].广州:华南理工大学,2011
[20]樊鹏程.新型自由基清除剂抗高原缺氧作用及保护机制研究[D].西安:第四军医大学,2013
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[3]苏平,宋思圆,吴秋敏,等.黄秋葵花黄酮及其酵母微胶囊对冷鲜肉的保鲜作用[J].中国食品学报,2016,16(06):139-144
[4]苏平,宋思圆,吴秋敏,等.黄秋葵花黄酮的酵母微胶囊制备及其对油脂的抗氧化能力[J].中国食品学报,2016,16(04):153-158
[5]程旺开,许月明,张冬冬.响应面优化黄秋葵叶多糖的提取工艺及其抗氧化活性考察[J].中国实验方剂学杂志,2017,23(04):38-42
[6]宋思圆,苏平,王丽娟,等.响应面试验优化超声提取黄秋葵花果胶多糖工艺及其体外抗氧化活性[J].食品学报,2017,38(02):283-289
[7]李孟秋,翟俊乐,田欢,等.黄秋葵提取物体外抗氧化活性的研究[J].中国食品添加剂,2015,(10):65-69
[8]徐康,杜金华.干燥方法对黄秋葵抗氧化能力的影响[J].食品与发酵工业,2016,42(05):120-125
[9] DUBOIS M,GILLES K A,HAMILTON J K,et al. Color-imetric method for determination of sugars and relatedsubstances[J]. Analytical Chemistry,1956,28(3):350-356
[10]李加兴,石春诚,马浪,等.黄秋葵果胶理化特性的研究[J].食品科学,2015,36(17):104-108
[11] TIAN F, LI B, JI B P, et al. Antioxidant and antimicrobial activities of consecutive extracts from Galla chinensis:The polarity affects the bioactivities[J]. Food Chemistry,2009,113(1):173-179
[12] WU P P,MA G Z,LI N H,et al. Investigation of invitroand in vivo antioxidant activities of flavonoids richextract from the berries of Rhodomyrtus tomentosa(Ait.)Hassk[J]. Food Chemistry,2015,173:194-202
[13] CHEN N F. Study on the extraction of flavonoid compound in pteridium aquilinum and it's antioxidant property[J]. Food and Fermentation Industries,2003,29(11):63-67
[14]潘瑶,郑时莲,邹兴平,等.葡萄、芒果、草莓乙醇提取物抗氧化活性组分分析及其抗氧化相互作用[J].食品科学,2017,(04):133-140
[15] PAN Y M, HE C H, WANG H S, et al. Antioxidant activity of microwave-assisted extract of Buddleia officinalisand its major active component[J]. Food Chemistry,2010,121(2):497-502
[16] YANG M, SHEN Q, LI L Q, et al. Phytochemical profiles, antioxidant activities of functional herb Abrus cantoniensis and Abrus mollis[J]. Food Chemistry,2015,177:304-312
[17]杜昕,李诚,肖岚.等.酶解牦牛血发酵液制备抗氧化肽工艺优化[J].核农学报,2017,31(02):325-333
[18]蔡为荣,孙元琳,汤坚.果胶多糖结构与降血脂研究进展[J].食品科学,2010,31(05):307-311
[19]任丹丹.黄秋葵多糖提取纯化及其体外结合胆酸能力和抑制肿瘤活性分析[D].广州:华南理工大学,2011
[20]樊鹏程.新型自由基清除剂抗高原缺氧作用及保护机制研究[D].西安:第四军医大学,2013