4个主要黄芪产地黄芪质量比较
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摘要
目的:比较4个主产地黄芪浸出物、总黄酮、毛蕊异黄酮葡萄糖苷、总皂苷及黄芪甲苷的含量差异,为种植、资源开发及合理使用提供参考依据。方法:应用浸出物测定法测定浸出物的含量;应用紫外可见分光光度法测定总黄酮、总皂苷的含量;应用高效液相色谱法测定毛蕊异黄酮葡萄糖苷、黄芪甲苷的含量。结果:黑龙江、甘肃、内蒙古和山西黄芪的冷浸法测定水溶性浸出物的含量分别为18.52%、21.13%、24.46%和19.33%;冷浸法测定醇溶性浸出物含量分别为8.11%、9.83%、11.44%和10.26%;总黄酮的含量分别为0.356%、0.574%、0.693%和0.492%;毛蕊异黄酮葡萄糖苷的含量分别为0.040%、0.111%、0.135%、0.021%;总皂苷的含量分别为0.171%、0.283%、0.342%、0.240%;黄芪甲苷的含量分别为0.042%、0.068%、0.079%、0.058%。结论:不同产地黄芪浸出物、总黄酮、毛蕊异黄酮葡萄糖苷、总皂苷、黄芪甲苷含量不同,黄芪水溶性浸出物含量内蒙古>甘肃>山西>黑龙江;醇溶性浸出物的含量内蒙古>山西>甘肃>黑龙江;总黄酮含量内蒙古>甘肃>山西>黑龙江;毛蕊异黄酮葡萄糖苷含量内蒙古>甘肃>黑龙江>山西;总皂苷的含量内蒙古>甘肃>山西>黑龙江;黄芪甲苷含量内蒙古>甘肃>山西>黑龙江。4个产地黄芪质量有较大差异,应建立多成分的评价指标,更科学地评价黄芪质量。
Objective: To provide the reference for the growth, resources development and rational utilization by comparing the differences existed in the contents of the extracts, total flavonoids, Isoflavone glucose, total saponins and astragaloside A contained in HuangQi from four major producing areas. Methods: The contents of the extracts were measured by extract determination method; the contents of total flavonoids and total saponins were determined by ultraviolet visible spectrophotometry; the contents of Isoflavone glucose and astragaloside A were detected by HPLC. Results: The contents of water soluble extractives collected from Heilongjiang, Gansu, Neimenggu and Shanxi Huangqi determined by cold soaking method were 18.52%, 21.13%, 24.46% and 19.33% respectively; the contents of alcohol-soluble extractive detected by cold soaking method were 8.11%, 9.83%, 11.44% and 10.26%respectively; the contents of total flavonoids were 0.356%, 0.574%, 0.693% and 0.492% respectively; the contents of Isoflavone glucose were 0.040%, 0.111%, 0.135% and 0.021%; the contents of total flavonoids were 0.171%,0.283%, 0.342% and 0.240%; the contents of astragaloside A were 0.042%, 0.068%, 0.079% and 0.058%. Conclusion:The contents of HuangQi extractives, total flavonoids, Isoflavone glucose, total saponins and astragaloside A are different, the contents of water soluble extractives are in the order: Neimenggu>Gansu>Shanxi>Heilongjiang; the contents of alcohol-soluble extractives rank: Neimenggu >Shanxi >Gansu >Heilongjiang; the contents of total flavonoids are in the order: Neimenggu>Gansu>Shanxi>Heilongjiang; the contents of Isoflavone glucose are in the order of Neimenggu>Gansu>Heilongjiang>Shanxi; the contents of Isoflavone glucose rank in the order: Neimenggu>Gansu >Shanxi >Heilongjiang; the contents of total saponins are in the order: Neimenggu >Gansu >Shanxi >Heilongjiang; the contents of astragaloside A rank in the order of Neimenggu>Gansu>Shanxi>Heilongjiang. There are the greater differences in the comparisons of the quality of HuangQi from four producing areas, the assessment indexes of many ingredients should be established to assess the quality of HuangQi more scientifically.
Objective: To provide the reference for the growth, resources development and rational utilization by comparing the differences existed in the contents of the extracts, total flavonoids, Isoflavone glucose, total saponins and astragaloside A contained in HuangQi from four major producing areas. Methods: The contents of the extracts were measured by extract determination method; the contents of total flavonoids and total saponins were determined by ultraviolet visible spectrophotometry; the contents of Isoflavone glucose and astragaloside A were detected by HPLC. Results: The contents of water soluble extractives collected from Heilongjiang, Gansu, Neimenggu and Shanxi Huangqi determined by cold soaking method were 18.52%, 21.13%, 24.46% and 19.33% respectively; the contents of alcohol-soluble extractive detected by cold soaking method were 8.11%, 9.83%, 11.44% and 10.26%respectively; the contents of total flavonoids were 0.356%, 0.574%, 0.693% and 0.492% respectively; the contents of Isoflavone glucose were 0.040%, 0.111%, 0.135% and 0.021%; the contents of total flavonoids were 0.171%,0.283%, 0.342% and 0.240%; the contents of astragaloside A were 0.042%, 0.068%, 0.079% and 0.058%. Conclusion:The contents of HuangQi extractives, total flavonoids, Isoflavone glucose, total saponins and astragaloside A are different, the contents of water soluble extractives are in the order: Neimenggu>Gansu>Shanxi>Heilongjiang; the contents of alcohol-soluble extractives rank: Neimenggu >Shanxi >Gansu >Heilongjiang; the contents of total flavonoids are in the order: Neimenggu>Gansu>Shanxi>Heilongjiang; the contents of Isoflavone glucose are in the order of Neimenggu>Gansu>Heilongjiang>Shanxi; the contents of Isoflavone glucose rank in the order: Neimenggu>Gansu >Shanxi >Heilongjiang; the contents of total saponins are in the order: Neimenggu >Gansu >Shanxi >Heilongjiang; the contents of astragaloside A rank in the order of Neimenggu>Gansu>Shanxi>Heilongjiang. There are the greater differences in the comparisons of the quality of HuangQi from four producing areas, the assessment indexes of many ingredients should be established to assess the quality of HuangQi more scientifically.
引文
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[6]Qi LW,Yu QT,Li P,et al.Quality evaluation of radix astragali through a simultaneous determination of six major active isoflavonoids and four main saponins by highperformance liquid chromatography coupled with diode array and evaporative light scattering detectors[J].J Chromatogr A,2006,1134(1/2):162-169.
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[8]刘冬莲.黄芪道地产区与非道地产区药材质量比较研究[J].唐山师范学院学报,2010,32(2):24-26.
[9]李春红,田吉,何兵,等.紫外分光光度法测定黄芪总黄酮的含量[J].重庆医科大学学报,2011,36(8):954-956.
[10]张军武,赵琦,王昌利.不同生长年限黄芪中黄芪总黄酮含量考察[J].西部中医药,2012,25(12):18-19.
[11]刘杨,包华音,王晓燕.不同产地黄芪药材中毛蕊异黄酮葡萄糖苷的定量分析[J].现代中药研究与实践,2014,28(3):8-10.
[12]滕红梅,昝亚玲,贾鹏,等.不同产地黄芪药材中毛蕊异黄酮葡萄糖苷含量的比较[J].江苏农业科学,2016,44(4):226-228.
[13]祝琳,王雪彦,晁若冰.比色法测定黄芪中总皂苷的含量[J].药物分析杂志,2009,29(7):1155-1158.
[14]李春红,田吉,张金平,等.比色法测定黄芪总皂苷的含量[J].泸州医学院学报,2011,34(4):349-351.
[15]刘和平,彭招华,张润容,等.黄芪药材中黄芪甲苷UPLC-ELSD含量测定方法的优化[J].中国实验方剂学杂志,2015,21(5):92-94.
[16]荀其宁,侯倩倩,刘志娟,等.蒸发光散射高效液相色谱法测定黄芪饮片中的黄芪甲苷含量[J].化学分析计量,2015,24(5):62-64.
[2]张银娣,王幼林,沈建平,等.黄芪皂苷甲的抗炎和降压作用[J].药学学报,1984,19(5):333-337.
[3]余灏,杨胜华.黄芪皂苷分析方法研究近况[J].华西药学杂志,1993,8(3):163-166.
[4]秦海林,赵天增,高令杰,等.人参皂甙与黄芪甲甙的薄层色谱分析[J].中国中药杂志,1995,20(4):226-227.
[5]梁连生,余静.黄芪中黄酮化合物的药理作用[J].中西医结合心脑血管病杂志,2005,3(12):1085-1087.
[6]Qi LW,Yu QT,Li P,et al.Quality evaluation of radix astragali through a simultaneous determination of six major active isoflavonoids and four main saponins by highperformance liquid chromatography coupled with diode array and evaporative light scattering detectors[J].J Chromatogr A,2006,1134(1/2):162-169.
[7]李桂春,高天爱,文建民,等.黄芪、红芪及其类同品的质量考察[J].中国中药杂志,1992,17(8):454-456.
[8]刘冬莲.黄芪道地产区与非道地产区药材质量比较研究[J].唐山师范学院学报,2010,32(2):24-26.
[9]李春红,田吉,何兵,等.紫外分光光度法测定黄芪总黄酮的含量[J].重庆医科大学学报,2011,36(8):954-956.
[10]张军武,赵琦,王昌利.不同生长年限黄芪中黄芪总黄酮含量考察[J].西部中医药,2012,25(12):18-19.
[11]刘杨,包华音,王晓燕.不同产地黄芪药材中毛蕊异黄酮葡萄糖苷的定量分析[J].现代中药研究与实践,2014,28(3):8-10.
[12]滕红梅,昝亚玲,贾鹏,等.不同产地黄芪药材中毛蕊异黄酮葡萄糖苷含量的比较[J].江苏农业科学,2016,44(4):226-228.
[13]祝琳,王雪彦,晁若冰.比色法测定黄芪中总皂苷的含量[J].药物分析杂志,2009,29(7):1155-1158.
[14]李春红,田吉,张金平,等.比色法测定黄芪总皂苷的含量[J].泸州医学院学报,2011,34(4):349-351.
[15]刘和平,彭招华,张润容,等.黄芪药材中黄芪甲苷UPLC-ELSD含量测定方法的优化[J].中国实验方剂学杂志,2015,21(5):92-94.
[16]荀其宁,侯倩倩,刘志娟,等.蒸发光散射高效液相色谱法测定黄芪饮片中的黄芪甲苷含量[J].化学分析计量,2015,24(5):62-64.
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