不同产地连翘药材指纹图谱的研究
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摘要
建立连翘药材的HPLC指纹图谱,为连翘药材质量的评价提供实验依据。采集不同产地的连翘药材,对生晒品、蒸品、煮品三种产地加工处理的三批连翘药材进行HPLC检测,建立以连翘苷为内标参照峰的连翘药材HPLC指纹图谱。按照建立的HPLC色谱条件,对6批市售药材进行检测,验证所得HPLC指纹图谱的实用性。三种产地加工品的HPLC图谱相似,建立了以连翘苷为内标参照峰的连翘药材HPLC指纹图谱。总共标定了20个共有峰,且共有峰面积均占总峰面积的90%以上。结果显示,6批市售药材与已知HPLC对照指纹图谱的相似度均>0.90。本研究确定的连翘HPLC指纹图谱对连翘药材的鉴别具有很好的适用性、推广性。
To establish the High performance liquid chromatography( HPLC) fingerprints on Forsythiae Fructus. Established the HPLC fingerprints on Forsythiae Fructus in three different kinds of field processed. Appraised the practicability of the HPLC fingerprints through comparison of corresponding fingerprints of commercial Forsythiae Fructus and common adulterants. The HPLC fingerprint with Phillyrin as internal standard reference peak of Forsythiae Fructus was established. 20 common characteristic peaks were determined,and the common peaks area accounted for more than 90% of the total area. ommercial Forsythiae Fructus and fruit of Forsythia viridissima Lindl were analyzed and identified by genuine Forsythiae Fructus HPLC fingerprint established in the study. The research method was scientific and practical verified from two aspects of positive and negative. The HPLC fingerprint of Forsythiae Fructus determined by this study has good applicability and popularization for the identification of Forsythiae Fructus.
To establish the High performance liquid chromatography( HPLC) fingerprints on Forsythiae Fructus. Established the HPLC fingerprints on Forsythiae Fructus in three different kinds of field processed. Appraised the practicability of the HPLC fingerprints through comparison of corresponding fingerprints of commercial Forsythiae Fructus and common adulterants. The HPLC fingerprint with Phillyrin as internal standard reference peak of Forsythiae Fructus was established. 20 common characteristic peaks were determined,and the common peaks area accounted for more than 90% of the total area. ommercial Forsythiae Fructus and fruit of Forsythia viridissima Lindl were analyzed and identified by genuine Forsythiae Fructus HPLC fingerprint established in the study. The research method was scientific and practical verified from two aspects of positive and negative. The HPLC fingerprint of Forsythiae Fructus determined by this study has good applicability and popularization for the identification of Forsythiae Fructus.
引文
[1]王夏炎.现代分析技术在中药指纹图谱研究中的应用[J].中草药,2004,35(7):127.
[2]史磊,张天锡,杜聪颖,等.中药全蝎活性成分、药理作用及临床应用研究进展[J].辽宁中医药大学学报,2015,17(4):89-91.
[3]国家药典委员会.中华人民共和国药典一部[M].北京:人民卫生出版社,2015.
[4]冯帅,王晓燕,李峰.山东产连翘HPLC指纹图谱的建立[J].山东中医药大学学报,2013,37(5):442-444.
[5]赵超,郑伶俐,胡亚刚,等.陕西道地药材连翘鲜品蒸煮工艺研究[J].时珍国医国药,2011,22(2):438-440.
[6]冯帅.基于化学-生物指纹图谱技术的连翘药材质量评价与品-质相关研究[D].济南:山东中医药大学,2014.
[7]冯帅,王晓燕,李峰.不同产地连翘的连翘苷及连翘酯苷A的含量比较[J].山东中医药大学学报,2013,37(6):514-515.
[8]孔晶晶,朱晶晶,王智民,等.一测多评法测定连翘中多种不同类型成分的含量[J].中国药学杂志,2010,45(17):1301-1304.
[9]付云飞,李清,毕开顺.RP-HPLC法同时测定不同产地连翘中的7种成分[J].中草药,2013(8):1043-1046.
[10]崔洋,王巧,张兰桐,等.河北道地药材连翘的高效液相色谱指纹图谱研究[J].中草药,2010(2):297-301.
[11]尤春雪,张振秋,李峰,等.HPLC波长切换技术对葛根中8种成分的测定及指纹图谱研究[J].中草药,2013(5):616-621.
[11]王勇,梁琼麟,胡坪.色谱及相关技术在中药质量评价中的应用[J].色谱,2008,26(2):136.
[12]方玲,陈方亮,余翠琴,等.不同产地乌药的HPLC指纹图谱研究[J].中草药,2013(2):229-231.
[13]窦志华,乔进,卞理,等.指纹图谱与一测多评法相结合的大黄质量控制方法[J].中国药学杂志,2015(5):442-448.
[14]张茹萍,何昱,石森林,等.HPLC指纹图谱结合主成分分析评价不同产地雷公藤药材质量[J].中国现代应用药学,2014(11):1338-1344.
[15]徐利国.《神农本草经》中连翘一名兰花考证[J].时珍国医国药,2010,11(4):358.
[16]王宁.连翘的本草考证[J].中药材,2013,36(4):670-671.
[17]史磊,刘雯,张美玲,等.连翘化学成分、药理活性现代研究[J].辽宁中医药大学学报,2016(12):222-224.
[18]初成玉.化学指纹分析指导下的连翘化学成分分离[D].大连:大连医科大学,2015.
[2]史磊,张天锡,杜聪颖,等.中药全蝎活性成分、药理作用及临床应用研究进展[J].辽宁中医药大学学报,2015,17(4):89-91.
[3]国家药典委员会.中华人民共和国药典一部[M].北京:人民卫生出版社,2015.
[4]冯帅,王晓燕,李峰.山东产连翘HPLC指纹图谱的建立[J].山东中医药大学学报,2013,37(5):442-444.
[5]赵超,郑伶俐,胡亚刚,等.陕西道地药材连翘鲜品蒸煮工艺研究[J].时珍国医国药,2011,22(2):438-440.
[6]冯帅.基于化学-生物指纹图谱技术的连翘药材质量评价与品-质相关研究[D].济南:山东中医药大学,2014.
[7]冯帅,王晓燕,李峰.不同产地连翘的连翘苷及连翘酯苷A的含量比较[J].山东中医药大学学报,2013,37(6):514-515.
[8]孔晶晶,朱晶晶,王智民,等.一测多评法测定连翘中多种不同类型成分的含量[J].中国药学杂志,2010,45(17):1301-1304.
[9]付云飞,李清,毕开顺.RP-HPLC法同时测定不同产地连翘中的7种成分[J].中草药,2013(8):1043-1046.
[10]崔洋,王巧,张兰桐,等.河北道地药材连翘的高效液相色谱指纹图谱研究[J].中草药,2010(2):297-301.
[11]尤春雪,张振秋,李峰,等.HPLC波长切换技术对葛根中8种成分的测定及指纹图谱研究[J].中草药,2013(5):616-621.
[11]王勇,梁琼麟,胡坪.色谱及相关技术在中药质量评价中的应用[J].色谱,2008,26(2):136.
[12]方玲,陈方亮,余翠琴,等.不同产地乌药的HPLC指纹图谱研究[J].中草药,2013(2):229-231.
[13]窦志华,乔进,卞理,等.指纹图谱与一测多评法相结合的大黄质量控制方法[J].中国药学杂志,2015(5):442-448.
[14]张茹萍,何昱,石森林,等.HPLC指纹图谱结合主成分分析评价不同产地雷公藤药材质量[J].中国现代应用药学,2014(11):1338-1344.
[15]徐利国.《神农本草经》中连翘一名兰花考证[J].时珍国医国药,2010,11(4):358.
[16]王宁.连翘的本草考证[J].中药材,2013,36(4):670-671.
[17]史磊,刘雯,张美玲,等.连翘化学成分、药理活性现代研究[J].辽宁中医药大学学报,2016(12):222-224.
[18]初成玉.化学指纹分析指导下的连翘化学成分分离[D].大连:大连医科大学,2015.