复合益生菌制剂对肉仔鸡生产性能、血清生化指标和免疫功能的影响
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摘要
为了探讨以乳酸菌、枯草芽孢杆菌和酵母菌复合配制的益生菌制剂在肉仔鸡上的应用效果,试验选用健康的1日龄雄性AA肉仔鸡400只,随机分为5组,分别为基础日粮(对照组,A组),基础日粮中添加0.2%、0.4%、0.6%和0.8%的复合益生菌制剂(试验组B、C、D、E),试验为期42 d。结果表明,添加复合益生菌制剂试验组的平均日增重显著高于对照组(P <0.05),且以0.6%添加剂量的效果最佳(P <0.05),添加0.4%、0.6%和0.8%复合益生菌制剂试验组的平均日采食量显著高于对照组(P <0.05),料重比显著低于对照组(P <0.05);与对照组相比,日粮添加0.4%、0.6%和0.8%的复合益生菌制剂显著降低了肉仔鸡21日龄时血清中血糖水平(P <0.05),添加0.4%、0.6%的复合益生菌制剂还显著提高了肉仔鸡42日龄血清中总蛋白水平(P <0.05);此外,与对照组相比,0.4%、0.6%和0.8%的复合益生菌制剂显著提高了肉仔鸡21日龄和42日龄血清中免疫球蛋白G的水平(P <0.05)。综上所述,日粮中添加以乳酸菌、枯草芽孢杆菌和酵母菌配制的复合益生菌制剂可以显著提高肉仔鸡的平均日增重、降低料重比,改善生产性能,增强肉鸡的免疫功能,且以0.6%添加剂量的效果为最优。
In order to explore the application effect of probiotics formulated by lactic acid bacteria,Bacillus subtilis and yeast on broilers,400 healthy one-day-old male AA broilers were randomly divided into five treatment groups. They were fed with basal diet(control group,group A),basal diet supplemented with 0.2%,0.4%,0.6% and 0.8% compound probiotics,respectively(test group B,C,D,E). The experiment lasted for 42 days. The results showed that the average daily gain of the experimental group with compound probiotics was significantly higher than that of the control group(P <0.05),and the effect of 0.6% additive was the best(P <0.05). The average daily feed intake of the experimental group with 0.4%,0.6% and 0.8% compound probiotics was significantly higher than that of the control group(P <0.05),while the feed-gain ratio was significantly lower(P <0.05). Compared with the control group,dietary supplementation of 0.4%,0.6% and 0.8% compound probiotics significantly decreased serum glucose level at 21-day-old broilers(P <0.05),and supplementation of 0.4% and 0.6% compound probiotics significantly increased serum total protein level at 42-day-old broilers(P <0.05). In addition,compared with the control group,0.4%,0.6% and 0.8% compound probiotics significantly increased serum immunoglobulin G levels in 21-day-old and 42-day-old broilers(P <0.05). To sum up,the diet supplemented with compound probiotics formulated by lactic acid bacteria,Bacillus subtilis and yeast can significantly improve the average daily gain,reduce feedWeight ratio,improve production performance,and enhance the immune function of broilers,and the effect of 0.6% additive is the best.
In order to explore the application effect of probiotics formulated by lactic acid bacteria,Bacillus subtilis and yeast on broilers,400 healthy one-day-old male AA broilers were randomly divided into five treatment groups. They were fed with basal diet(control group,group A),basal diet supplemented with 0.2%,0.4%,0.6% and 0.8% compound probiotics,respectively(test group B,C,D,E). The experiment lasted for 42 days. The results showed that the average daily gain of the experimental group with compound probiotics was significantly higher than that of the control group(P <0.05),and the effect of 0.6% additive was the best(P <0.05). The average daily feed intake of the experimental group with 0.4%,0.6% and 0.8% compound probiotics was significantly higher than that of the control group(P <0.05),while the feed-gain ratio was significantly lower(P <0.05). Compared with the control group,dietary supplementation of 0.4%,0.6% and 0.8% compound probiotics significantly decreased serum glucose level at 21-day-old broilers(P <0.05),and supplementation of 0.4% and 0.6% compound probiotics significantly increased serum total protein level at 42-day-old broilers(P <0.05). In addition,compared with the control group,0.4%,0.6% and 0.8% compound probiotics significantly increased serum immunoglobulin G levels in 21-day-old and 42-day-old broilers(P <0.05). To sum up,the diet supplemented with compound probiotics formulated by lactic acid bacteria,Bacillus subtilis and yeast can significantly improve the average daily gain,reduce feedWeight ratio,improve production performance,and enhance the immune function of broilers,and the effect of 0.6% additive is the best.
引文
[1]白耀辉,杨晓虹,金露,等.益生菌制剂在肉鸡生产中的研究进展[J].畜牧与饲料科学,2011,2:80~82.
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[6]林谦,戴求仲,宾石玉,等.益生菌与酶制剂对黄羽肉鸡血液生化指标和免疫性能影响的协同效应研究[J].饲料工业,2012,33(14):31~36.
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[8]秦康乐.复合益生菌对肉种鸡生产性能的影响[D].扬州大学,2018.
[9]王晶,许丽,孙文,等.鸡源乳酸菌对雏鸡生长性能和免疫性能的影响[A].全国家禽科学学术讨论会[C].2009:975~980.
[10]王涛,任景乐,祝贵华,等.复合乳酸杆菌制剂对蛋种鸡产蛋性能、免疫机能和盲肠微生物的影响[J].动物营养学报,2013,25(7):1551~1558.
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[13]张彩凤,齐广海,王晓翠,等.乳酸菌和酵母菌复合制剂及其与维吉尼亚霉素联合使用对肉仔鸡血清生化指标、抗氧化能力和免疫功能的影响[J].动物营养学报,2018,5:1893~1901.
[14]张凯,杨志强,王学智,等.发酵型黄芪提取物对肉仔鸡生产性能及免疫球蛋白的作用研究[J].湖北农业科学,2011,50(6):1216~1218.
[15]张献月.乳酸菌和芽孢杆菌复合微生态制剂在肉仔鸡生产中的应用效果研究[D].江西农业大学,2013.
[16]赵建文.微生态制剂对鸡舍氨气和肉鸡免疫系统的影响[D].山东农业大学,2011.
[17]Jeong J S,Kim I H.Jeong J S,Kim I H. Effect of Bacillus subtilis C-3102 spores as a probiotic feed supplement on growth performance,noxious gas emission,and intestinal microflora in broilers[J].Poultry Science,2014,93(12):3097~3103.
[18]Shokryazdan P,Faseleh J M,Liang J B,et al. Effects of a Lactobacillus salivarius mixture on performance,intestinal health and serum lipids of broiler chickens[J].Plos One,2017,12(5):e0175959.
[19]Tana,Watarai S,Isogai E,et al. Induction of intestinal IgA and IgG antibodies preventing adhesion of verotoxin-producing Escherichi acoli to Caco-2 cells by oral immunization with liposomes[J].Letters in Applied Microbiology,2010,36(3):135~139.
[2]陈琼.中国肉鸡生产的成本收益与效率研究[D].中国农业科学院,2013.
[3]何欣,滑静,王晓霞,等.酵母盐对肉仔鸡生产性能、胫骨质量及血液生化指标的影响[J].北京农学院学报,2004,19(4):34~36.
[4]李亚杰,赵献军.益生菌·黄芪多糖微胶囊制剂对肉仔鸡生长性能·免疫器官指数及血液生化指标的影响[J].安徽农业科学,2007,35(1):100~103.
[5]林滉.乳酸菌素对肉鸡生产性能影响及作用机理的研究[D].福建农林大学,2011.
[6]林谦,戴求仲,宾石玉,等.益生菌与酶制剂对黄羽肉鸡血液生化指标和免疫性能影响的协同效应研究[J].饲料工业,2012,33(14):31~36.
[7]刘乃芝,陈静,崔诗法,等.不同浓度植物乳杆菌对肉鸡免疫机能的影响[J].吉林农业,2012,4:59~60.
[8]秦康乐.复合益生菌对肉种鸡生产性能的影响[D].扬州大学,2018.
[9]王晶,许丽,孙文,等.鸡源乳酸菌对雏鸡生长性能和免疫性能的影响[A].全国家禽科学学术讨论会[C].2009:975~980.
[10]王涛,任景乐,祝贵华,等.复合乳酸杆菌制剂对蛋种鸡产蛋性能、免疫机能和盲肠微生物的影响[J].动物营养学报,2013,25(7):1551~1558.
[11]谢全喜,崔诗法,徐海燕,等.复合微生态制剂与饲用抗生素对肉鸡生长性能、免疫性能和抗氧化指标的影响[J].动物营养学报,2012,24(7):1336~1344
[12]原立海,王安.低温和维生素A对育成蛋鸭生产性能及生化指标的影响[J].东北农业大学学报,2008,39(2):207~212.
[13]张彩凤,齐广海,王晓翠,等.乳酸菌和酵母菌复合制剂及其与维吉尼亚霉素联合使用对肉仔鸡血清生化指标、抗氧化能力和免疫功能的影响[J].动物营养学报,2018,5:1893~1901.
[14]张凯,杨志强,王学智,等.发酵型黄芪提取物对肉仔鸡生产性能及免疫球蛋白的作用研究[J].湖北农业科学,2011,50(6):1216~1218.
[15]张献月.乳酸菌和芽孢杆菌复合微生态制剂在肉仔鸡生产中的应用效果研究[D].江西农业大学,2013.
[16]赵建文.微生态制剂对鸡舍氨气和肉鸡免疫系统的影响[D].山东农业大学,2011.
[17]Jeong J S,Kim I H.Jeong J S,Kim I H. Effect of Bacillus subtilis C-3102 spores as a probiotic feed supplement on growth performance,noxious gas emission,and intestinal microflora in broilers[J].Poultry Science,2014,93(12):3097~3103.
[18]Shokryazdan P,Faseleh J M,Liang J B,et al. Effects of a Lactobacillus salivarius mixture on performance,intestinal health and serum lipids of broiler chickens[J].Plos One,2017,12(5):e0175959.
[19]Tana,Watarai S,Isogai E,et al. Induction of intestinal IgA and IgG antibodies preventing adhesion of verotoxin-producing Escherichi acoli to Caco-2 cells by oral immunization with liposomes[J].Letters in Applied Microbiology,2010,36(3):135~139.
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