不同种衣剂复配对烟草种子萌发及幼苗生理生化指标的影响
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摘要
[目的]更好地提高烟草种子包衣质量,满足烟苗的养分需求,培养壮苗、健苗。[方法]采用不同比例的硅藻土、膨润土、轻钙粉、复合微生物菌剂复配对K326种子进行包衣处理,统计分析不同包衣配方对烟草种子萌发率和各项生理生化指标变化的影响。[结果]烟草种子经配方C(硅藻土50%+膨润土30%+轻钙粉19.5%+复合微生物菌剂0.5%)和配方D(硅藻土60%+膨润土20%+轻钙粉19.5%+复合微生物菌剂0.5%)包衣处理后,萌发迅速且发芽率高,烟苗干物质含量增加,叶绿素含量增加,根系活力明显提高,过氧化物酶活性提高、丙二醛含量降低,幼苗抗逆性好,可以有效缓解烟苗生长过程中受到的环境胁迫。[结论]配方C和配方D包衣效果显著,可起到替代商品化包衣剂的作用。
[Objective] In order to improve the quality of tobacco seed coating,to meet the nutrient requirements of tobacco seedlings,and to cultivate strong and healthy seedlings.[Method]Different proportions of diatomite,bentonite,light calcium carbonate and compound microbial agents were used to compound K326 seeds for coating treatment in this study.The effects of different coating formulas on the germination rate and physiological and biochemical indexes of tobacco seeds were statistically analyzed.[Result]The results showed that after the coating treatment with formula C(diatomite 50%+bentonite 30%+light calcium carbonate 19.5%+compound microbial agent 0.5%) and formula D(diatomite 60% + bentonite 20% + light calcium carbonate 19.5%+compound microbial agent 0.5%) the tobacco seeds gained rapid germination and high germination rate.Besides,the dry matter content,the chlorophyll content,the root activity and the peroxidase activity of the tobacco seedlings increased significantly.On the other hand,the malondialdehyde content decreased,which showed that the seedlings had good stress resistance and could effectively alleviate the environmental stress on the growth of tobacco seedlings.[Conclusion]The two formulas had significant coating effects as alternatives to commercial coating agents.
[Objective] In order to improve the quality of tobacco seed coating,to meet the nutrient requirements of tobacco seedlings,and to cultivate strong and healthy seedlings.[Method]Different proportions of diatomite,bentonite,light calcium carbonate and compound microbial agents were used to compound K326 seeds for coating treatment in this study.The effects of different coating formulas on the germination rate and physiological and biochemical indexes of tobacco seeds were statistically analyzed.[Result]The results showed that after the coating treatment with formula C(diatomite 50%+bentonite 30%+light calcium carbonate 19.5%+compound microbial agent 0.5%) and formula D(diatomite 60% + bentonite 20% + light calcium carbonate 19.5%+compound microbial agent 0.5%) the tobacco seeds gained rapid germination and high germination rate.Besides,the dry matter content,the chlorophyll content,the root activity and the peroxidase activity of the tobacco seedlings increased significantly.On the other hand,the malondialdehyde content decreased,which showed that the seedlings had good stress resistance and could effectively alleviate the environmental stress on the growth of tobacco seedlings.[Conclusion]The two formulas had significant coating effects as alternatives to commercial coating agents.
引文
[1] 招启柏,黄年生,徐卯林.我国烟草丸粒化包衣技术的研究与发展方向[J].中国烟草科学,2002,23(1): 25-27.
[2] 李晓斌,魏成兵.硅藻土在废水处理中的应用[J].江苏环境科技,2008,21(2):71-74.
[3] 朱健,王平,李科林,等.硅藻土对污染土壤中铅的固定效果及机制的研究[J].中国农学通报,2012,28(14):240-245.
[4] 姜玉芝,贾嵩阳.硅藻土的国内外开发应用现状及进展[J].有色矿冶,2011,27(5):31-37.
[5] 谢正苗,俞天明,姜军涛.膨润土修复矿区污染土壤的初探[J].科技通报,2009,25(1):109-113.
[6] 胡克伟,贾冬艳,颜丽,等.膨润土对重金属离子的竞争性吸附研究[J].土壤通报,2011,42(2):467-470.
[7] 陈刚,李现道,许家来,等.一种复合微生物肥料:CN105085121A[P].2015-11-25.
[8] ZHANG S,HU J,ZHANG C Y,et al.Seed priming with brassinolide improves lucerne (Medicago sativa L.) seed germination and seedling growth in relation to physiological changes under salinity stress [J].Australian journal of agricultural research,2007,58(8): 811-815.
[9] QIU J,WANG R M,YAN J Z,et al.Seed film pelleting with uniconazole improves rape seedling growth in relation to physiological changes under waterlogging stress [J].Plant growth regulation,2005,47(1): 75-81.
[10] 国家烟草质量监督检验中心.烟草及烟草制品总氮的测定连续流动法:YC/T 161—2002[S].北京:商务印书馆,2002.
[11] 隋方功,李俊良,李旭霖.土壤农化分析实验[M].青岛:青岛农业大学,2017.
[12] 国家烟草专卖局.烟草及烟草制品钾的测定连续流动法:YC/T 217—2007[S].北京:中国标准出版社,2007.
[13] 刘家尧,刘新.植物生理学实验教程[M].北京:高等教育出版社,2010.
[14] 丛松峰.生物化学实验[M].上海:上海交通大学出版社,2005: 91-93.
[15] 魏群.基础生物化学实验[M].北京:高等教育出版社,2009.
[16] 史新,徐应明,谢忠雷,等.膨润土对镉胁迫下水稻幼苗生理生化特性的影响[J].生态与农村环境学报,2012,28(6):687-693.
[17] 王治,马小凡,孙炎焱,等.紫花苜蓿和沙打旺包衣配方研究[J].草业科学,2006,23(7):28-31.
[18] 张金香,武亚敬,史靖,等.油松种子丸化包衣材料的筛选[J].种子,2009,28(8):90-92.
[19] 梁猛,李绍才,龙凤,等.胡枝子和紫穗槐包衣配方研究[J].种子,2014,33(4):69-71.
[20] 徐培洲,李云,袁澍,等.叶绿素缺乏水稻突变体中光系统蛋白和叶绿素合成特性的研究[J].中国农业科学,2006,39(7):1299-1305.
[21] 曹宗巽.根系在植物生活中的作用[J].生物学通报,1959(10):488-492.
[22] 巩振辉.PVA渗调对线椒种子活力及芽期酶活性的影响[J].河南职技师院学报,1995,23(2):29-32.
[23] 赵剑,王俭荣,杨文杰.聚乙二醇和聚乙烯醇渗调处理对苜蓿幼苗的抗冷害的影响[J].东北师范大学学报(自然科学版),1998(2):50-54.
[24] 张海旺,芦翠乔,吴丁,等.聚乙二醇(PEG)渗透处理对老化油菜种子过氧化及细胞膜透性的影响[J].华北农业学报,1989,4(2):56-62.
[25]张燕,方力,李樱.H2O2对提高烟草种子活力效应的研究[J].农业与技术,1997(4):42-44.
[2] 李晓斌,魏成兵.硅藻土在废水处理中的应用[J].江苏环境科技,2008,21(2):71-74.
[3] 朱健,王平,李科林,等.硅藻土对污染土壤中铅的固定效果及机制的研究[J].中国农学通报,2012,28(14):240-245.
[4] 姜玉芝,贾嵩阳.硅藻土的国内外开发应用现状及进展[J].有色矿冶,2011,27(5):31-37.
[5] 谢正苗,俞天明,姜军涛.膨润土修复矿区污染土壤的初探[J].科技通报,2009,25(1):109-113.
[6] 胡克伟,贾冬艳,颜丽,等.膨润土对重金属离子的竞争性吸附研究[J].土壤通报,2011,42(2):467-470.
[7] 陈刚,李现道,许家来,等.一种复合微生物肥料:CN105085121A[P].2015-11-25.
[8] ZHANG S,HU J,ZHANG C Y,et al.Seed priming with brassinolide improves lucerne (Medicago sativa L.) seed germination and seedling growth in relation to physiological changes under salinity stress [J].Australian journal of agricultural research,2007,58(8): 811-815.
[9] QIU J,WANG R M,YAN J Z,et al.Seed film pelleting with uniconazole improves rape seedling growth in relation to physiological changes under waterlogging stress [J].Plant growth regulation,2005,47(1): 75-81.
[10] 国家烟草质量监督检验中心.烟草及烟草制品总氮的测定连续流动法:YC/T 161—2002[S].北京:商务印书馆,2002.
[11] 隋方功,李俊良,李旭霖.土壤农化分析实验[M].青岛:青岛农业大学,2017.
[12] 国家烟草专卖局.烟草及烟草制品钾的测定连续流动法:YC/T 217—2007[S].北京:中国标准出版社,2007.
[13] 刘家尧,刘新.植物生理学实验教程[M].北京:高等教育出版社,2010.
[14] 丛松峰.生物化学实验[M].上海:上海交通大学出版社,2005: 91-93.
[15] 魏群.基础生物化学实验[M].北京:高等教育出版社,2009.
[16] 史新,徐应明,谢忠雷,等.膨润土对镉胁迫下水稻幼苗生理生化特性的影响[J].生态与农村环境学报,2012,28(6):687-693.
[17] 王治,马小凡,孙炎焱,等.紫花苜蓿和沙打旺包衣配方研究[J].草业科学,2006,23(7):28-31.
[18] 张金香,武亚敬,史靖,等.油松种子丸化包衣材料的筛选[J].种子,2009,28(8):90-92.
[19] 梁猛,李绍才,龙凤,等.胡枝子和紫穗槐包衣配方研究[J].种子,2014,33(4):69-71.
[20] 徐培洲,李云,袁澍,等.叶绿素缺乏水稻突变体中光系统蛋白和叶绿素合成特性的研究[J].中国农业科学,2006,39(7):1299-1305.
[21] 曹宗巽.根系在植物生活中的作用[J].生物学通报,1959(10):488-492.
[22] 巩振辉.PVA渗调对线椒种子活力及芽期酶活性的影响[J].河南职技师院学报,1995,23(2):29-32.
[23] 赵剑,王俭荣,杨文杰.聚乙二醇和聚乙烯醇渗调处理对苜蓿幼苗的抗冷害的影响[J].东北师范大学学报(自然科学版),1998(2):50-54.
[24] 张海旺,芦翠乔,吴丁,等.聚乙二醇(PEG)渗透处理对老化油菜种子过氧化及细胞膜透性的影响[J].华北农业学报,1989,4(2):56-62.
[25]张燕,方力,李樱.H2O2对提高烟草种子活力效应的研究[J].农业与技术,1997(4):42-44.
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